Análise da expressão do mRNA da proteína S100β em adipócitos de pacientes com diabetes melito tipo 2

Abstract

OBJETIVO: O presente trabalho objetiva compreender a possível relação do nível de expressão gênica do mRNA da proteína S100β em adipócitos com o diabetes melito do tipo 2, pela comparação de dados de portadores dessa doença com os de indivíduos normoglicêmicos. MATERIAIS E MÉTODOS: Foram selecionadas amostras de tecido adiposo de oito pacientes da Seção de Coronárias do Instituto Dante Pazzanese de Cardiologia (IDPC), sendo quatro do grupo diabetes e quatro do grupo de normoglicêmicos. Essas amostras foram submetidas à técnica de RT-PCR em tempo real. RESULTADOS: Por meio do Test-t de Student para os valores de diferença entre os ciclos threshold (ΔCt), observou-se que houve aumento de aproximadamente 15 vezes (p = 0,015) da expressão do mRNA da proteína S100β nos adipócitos dos indivíduos do grupo diabetes quando comparado aos do grupo controle. CONCLUSÃO: Nossos resultados evidenciam, de forma inédita, coexistência entre o aumento da expressão do gene S100β e a patologia do diabetes melito do tipo 2.

Prognostic values of S100 family members in ovarian cancer patients

“…Additionally, the expression of RAGE and EN-RAGE, the downstream effectors of S100A12, was significantly increased, as evidenced by the significantly greater mRNA and protein expression in the cells of the endometriosis patients [77]. As to hepatic or renal illnesses and diabetes, S100A4 promotes liver fibrosis by activating HSCs (hepatic stellate cell) [78]; serum concentrations of S100B are higher in patients with cirrhosis than in healthy volunteers; silencing S100A9 clearly alleviated G-MDSCs (Granuloid medullary sources inhibit cells) expansion and hepatic steatosis caused by ATF3 deficiency or GC (glucocorticoid) treatment [79]; the sunitinib may protect against renal damage from diabetes mellitus through regulating the levels of vimentin, E-cadherin and S100 [80]; there is coexistence of increased expression of the S100B and the type 2 diabetes mellitus gene in patients [81]; patients with type 2 diabetes have reticulated thrombocytosis that correlates with glycated hemoglobin as well as increased plasma S100A8/A9 levels [82]; S100A8/A9 regulates renal damage and fibrosis, probably through loss of tubular epithelial cell contacts and irreversible damage [83]; higher urine S100 levels are associated with increased lupus nephritis activity in childhood-onset systemic lupus erythematosus, whereas serum S100 (S100A4, S100A6, S100A8/9, and S100A12) levels do not correlate with disease activity [84]; high S100A12 levels are associated with the presence and severity of coronary artery disease in patients with T2DM (type 2 diabetes mellitus) [85].…”