Butyrylcholinesterase and diabetes mellitus in the CHE2 C5- and CHE2 C5+ phenotypes

Cwiertnia, Mauro M.; Alcântara, Vânia M.; Réa, Rosângela Roginski; Faria, Ana Cristina R. A.; Picheth, Geraldo; Scartezini, Mariléia; Graef, Lorena E.; Welter, Marciane

doi:10.1590/s0004-27302010000100011

Cited by 8 publications

(7 citation statements)

References 40 publications

(51 reference statements)

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“…8 show that there was no significant difference in the gross serum butyrylcholinesterase activity between the diabetic patients and the control subjects. This result is in agreement with certain previous findings that serum butyrylcholinesterase activity was not elevated in diabetic patients when compared with healthy subjects [54,55]. …”

Section: Resultssupporting

confidence: 94%

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Nongradient blue native gel analysis of serum proteins and in-gel detection of serum esterase activities

Thangthaeng

Sumien

Forster

et al. 2011

Journal of Chromatography B

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The objective of the present study was to analyze serum protein complexes and detect serum esterase activities using nongradient blue native polyacrylamide gel electrophoresis (BN-PAGE). For analysis of potential protein complexes, serum from rat was used. Results demonstrate that a total of 8 gel bands could be clearly distinguished after Coomassie blue staining, and serum albumin could be isolated nearly as a pure protein. Moreover, proteins in these bands were identified by electrospray mass spectrometry and low-energy collision induced dissociation (CID)-MS/MS peptide sequencing and the existence of serum dihydrolipoamide dehydrogenase (DLDH) was confirmed. For studies of in-gel detection of esterase activities, serum from rat, mouse, and human was used. In-gel staining of esterase activity was achieved by the use of either α-naphthylacetate or β-naphthylacetate in the presence of Fast blue BB salt. There were three bands exhibiting esterase activities in the serum of both rat and mouse. In contrast, there was only one band showing esterase activity staining in the human serum. When serum samples were treated with varying concentrations of urea, esterase activity staining was abolished for all the bands except the one containing esterase 1 (Es1) protein that is known to be a single polypeptide enzyme, indicating that majority of these esterases were protein complexes or multimeric proteins. We also identified the human serum esterase as butyrylcholinesterase following isolation and partial purification using ammonium sulfate fractioning and ion exchange column chromatographies. Where applicable, demonstrations of the gel-based method for measuring serum esterase activities under physiological or pathophysiological conditions were illustrated. Results of the present study demonstrate that nongradient BN-PAGE can serve as a feasible analytical tool for proteomic and enzymatic analysis of serum proteins.

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Section: Resultssupporting

confidence: 94%

“…8). While there are reports in the literature describing similar findings to ours [54,55], there are also studies reporting elevated levels of butyrylcholinesterase activities in diabetic patients [70,71]. The reason for this discrepancy remains unknown, but it is likely that detectable changes may depend on the severity of the disease.…”

Section: Discussionsupporting

confidence: 69%

Nongradient blue native gel analysis of serum proteins and in-gel detection of serum esterase activities

Thangthaeng

Sumien

Forster

et al. 2011

Journal of Chromatography B

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“…Although previous studies have shown a positive correlation between BuChE activity and glucose levels (Cwiertnia et al, 2010;Jabeen et al, 2014) this study showed significant correlation (P <0.05) with liver glycogen. Although the mechanism is unknown, evidence of relationship between the activity of BuChE and carbohydrate metabolism in liver and plasma of C. gariepinus could serve as early warning of health disorder (Thiermann et al, 2007;Cwiertnia et al, 2010;Benyamin et al, 2011;Santarpia et al, 2013).…”

Section: Plasma Glucose and Liver Glycogen Responsescontrasting

confidence: 91%

“…Although the mechanism is unknown, evidence of relationship between the activity of BuChE and carbohydrate metabolism in liver and plasma of C. gariepinus could serve as early warning of health disorder (Thiermann et al, 2007;Cwiertnia et al, 2010;Benyamin et al, 2011;Santarpia et al, 2013).…”

Section: Plasma Glucose and Liver Glycogen Responsesmentioning

confidence: 99%

Butrylacetycholinesterase Activity in Liver and Plasma, Liver Glycogen and Plasma Glucose Content, Haematology and Behaviour of Clariid Catfish Clarias Gariepinus to Dichlorvos

Ezike

Felix

Ajima

et al. 2017

IJAFAS

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Semi-static bioassay experiment was conducted to ascertain Butrylacetycholinesterase enzyme activity in liver and plasma, liver glycogen and plasma glucose content, haematology as well as behaviour of Clarias gariepinus (mean weight 24.52±1.64 g) to varying concentrations (0, 0.4, 0.8, 1.6 mg L -1 ) of Dichlorvos for 96 hours. Butrylacetycholinesterase activity in liver was significantly (P <0.05) inhibited all through the exposure duration to 0.4, 0.8 and 1.6 mg L -1 of DDVP compared with control group of fish. Similarly, plasma BuChE activity was inhibited all through the exposure duration to 1.6 mg L -1 DDVP compared to control. There was a significant (P <0.05) inhibition in HB, RBC and MCV of exposed fish after 24,48, 72 and 96h to 1.6 mg L -1 DDVP compared to the control but PCV and WBC were significantly elevated after 72 and 96h to 1.6 mg L -1 DDVP compared to the control.Plasma cortisol and liver glycogen were significantly inhibited after 72 and 96 h to 0.8 and 1.6 mg L

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“…This difference was not observed among patients with the CHE2 C5+ phenotype. Also for patients with CHE2 C5− phenotype and obesity higher BChE activity was observed compared to nonobese patients [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in Cholinesterase Activity in Blood of Adolescent with Metabolic Syndrome after Supplementation with Extract fromAronia melanocarpa

Duchnowicz

Ziobro

Rapacka

et al. 2018

BioMed Research International

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Obesity and metabolic syndrome (MetS) are growing problems among children and adolescents. There are no reports of changes in the activity of butyrylcholinesterase (BChE) in children and adolescents with metabolic syndrome especially after supplementation with extract from Aronia melanocarpa. Materials studied included plasma and erythrocytes isolated from peripheral blood of patients with MetS and healthy subjects. We have estimated the following parameters: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity, lipid peroxidation and lipids levels in plasma, and erythrocytes membrane. In patients with MetS, a significant increase in AChE and BChE activity, higher LDL-cholesterol and triacylglycerol levels, and lower HDL-cholesterol level were observed. Supplementation with A. melanocarpa extract resulted in mild but statistically significant reduction of total cholesterol, LDL-cholesterol, and triacylglycerol levels and caused an increase in HDL-cholesterol level and a decrease in lipid peroxidation in plasma patients with MetS. Additionally, a decrease in lipid peroxidation and cholesterol level and a decrease in AChE activity in the erythrocyte membranes after supplementation with A. melanocarpa were noted. Summarizing, an increase in AChE and BChE activity and disruption of lipid metabolism in patients with MetS were observed. After supplementation of MetS patients with A. melanocarpa extract, a decrease in AChE activity and oxidative stress was noted.

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Butyrylcholinesterase and diabetes mellitus in the CHE2 C5- and CHE2 C5+ phenotypes

Cited by 8 publications

References 40 publications

Nongradient blue native gel analysis of serum proteins and in-gel detection of serum esterase activities

Nongradient blue native gel analysis of serum proteins and in-gel detection of serum esterase activities

Butrylacetycholinesterase Activity in Liver and Plasma, Liver Glycogen and Plasma Glucose Content, Haematology and Behaviour of Clariid Catfish Clarias Gariepinus to Dichlorvos

Changes in Cholinesterase Activity in Blood of Adolescent with Metabolic Syndrome after Supplementation with Extract fromAronia melanocarpa

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