Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells

Schwindt, Telma Tiemi; Motta, Fabiana Louise; F, Barnabé Gabriela; G, Massant Cristina; Guimaraes, Alessander O.; Calcagnotto, María Elisa; Pesquero, João Bosco; Mello, Luiz E.

doi:10.1590/s0001-37652009000300009

Cited by 32 publications

(19 citation statements)

References 27 publications

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“…Neural differentiation of placental stem cells with RA plus hBDNF resulted in 5-20 % of oligodendrocytic cells (Portmann-Lanz et al 2010). It has been reported that bFGF increased the proliferative potential of MSCs (Schwindt et al 2009;Sotiropoulou et al 2006). We suggest that the increased cell density with FGF and EGF can lead to cells with a higher positive CNPase level.…”

Section: Discussionmentioning

confidence: 65%

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Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

et al. 2014

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Many neural disorders are characterized by the loss of one or several types of neural cells. Human umbilical cord-derived mesenchymal cells (hUCMs) are capable of differentiating into neuron, astroglia-like and oligodendrocyte cell types. However, a reliable means of inducing the selective differentiation of hUCMs into neural cells in vitro has not yet been established. For induction of neural differentiation, hUCMs were seeded onto sterile glass slides and six various cocktails using a base medium (DMEM/LG) supplemented with 10 % FBS, retinoic acid (RA), dimethyl sulfoxide (DMSO), epidermal growth factor (EGF) and fibroblast growth factor (FGF) were used to compare their effect on neuronal, astrocyte and oligodandrocyte differentiation. The hUCMs were positive for mesenchymal markers, while they were negative for hematopoietic markers. Differentiation to adipogenic and osteogenic lineage was detected in these cells. Our data revealed that the cocktail consisting of DMEM/LG, FBS, RA, FGF, and EGF (DF/R/Fg/E group) induced hUCM cells to express the highest percentage of nestin, ß-tubulin III, neurofilament, and CNPase. The DF/Ds/Fg/E group led to the highest percentage of GFAP expression. While the expression levels of NF, GFAP, and CNPase were the lowest in the DF group. The least percentage of nestin and ß-tubulin III expression was observed in the DF/Ds group. We may conclude that FGF and EGF are important inducers for differentiation of hUCMs into neuron, astrocyte and oligodendrocyte. RA can induce hUCMs to differentiate into neuron and oligodendrocyte while for astrocyte differentiation DMSO had a pivotal role.

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Section: Discussionmentioning

confidence: 65%

“…The results from this study showed that the proportion of GFAP-positive cells in untreated cells was less than induced cells in DF/Ds/Fg/E group. GFAP can also be expressed by immature neural progenitor cells (Schwindt et al 2009). Long-term (14 days) exposure to RA dramatically reduced the GFAP-positive cells.…”

Section: Discussionmentioning

confidence: 99%

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

et al. 2014

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“…These cellular structures represent three dimensional heterogeneous cell clusters, comprising stem cells, committed progenitors, as well as differentiated neural cells [7][8][9] . As growth factors maintain the proliferative characteristics of NS, their withdrawal will stop the proliferating process and will start the differentiation of cells [10] , leading to an increase in markers of fully differentiated neural cell populations, such as neurons and glial cells [7,11] . With the advances in stem cell biology, a debate has emerged regarding the origin of tumors, particularly gliomas.…”

Section: Introductionmentioning

confidence: 99%

Early Differentiating Mouse Astroglial Progenitors Share Common Protein Signatures with GL261 Glioma Cells

Brites¹

2016

JSRB

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Neural stem cells (NSC) biology is being applied to tumor research because these cells have been shown to share key properties with cancer cells. Gliomas represent the most common brain tumor, and neural stem/progenitor cells (NSPC) or early-differentiated cell type lineages may be on its origin. Here, we identified the developmental stage of the differentiation process of NSC into astrocytes that showed the highest number of tumorigenic similarities.NSPC were grown as neurospheres and astroglial differentiation was induced during 7 days in vitro (DIV). Cellular characterization was evaluated by specific neural markers at two developmental windows, i.e. NSPC/astrocyte progenitors from neurospheres until 3 DIV, and differentiating astrocytes thereafter. Predominance of immature Sox2-positive cells was verified in the first window and a prevalence of GFAP-positive cells in the second one. We then compared some tumor-related markers in GL261 glioma cells with such differentiating periods. The early progenitor cells (until 2 DIV) were those with the closest resemblances to the glioma cell line regarding BrdU incorporation, expression of microtubule-associated protein light chain 3 and of angiogenic factors (VEGF/VEGFR2), as well as S100B release. Our results suggest that early differentiating astroglial progenitors may be more susceptible to malignant transformation.

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“…FGF-2 has been found to be a potent modulator of proliferation in culture of neonatal and adult precursor cells, supporting strong role of this factor in neurogenesis (Ford-Perriss et al 2001). Yet, FGF-2 mechanisms of cell signaling in neurospheres growth are still unclear (Schwindt et al 2009). It has been demonstrated that FGF-2 increases neural stem cell proliferation by upregulating of a VEGF receptor expression via stimulating phosphorylation of extracellular-regulated kinases Xiao et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Induction of Perlecan Expression and Neural Cell Proliferation by FGF-2 in the Developing Cerebral Cortex: An In Vivo Study

2010

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Fibroblast growth factor has been implicated in cerebral cortical cell proliferation, migration, survival and differentiation. In the nervous system, proteoglycans (PGs) modulate growth factor activities and cell proliferation. Perlecan is a large PG and is expressed during the prenatal stages of brain development. Perlecan can promote fibroblast growth factor-2 (FGF-2) activity. In this study the effects of FGF-2 on neurogenesis and perlecan expression in the cerebral cortex have been studied. FGF-2 and anti-FGF-2 antibody were administered to 1-day-old mouse pups through the cisterna magna. Two days after injection, the pups were harvested and brains were removed for further analysis. We used western blotting and immunohistochemistry in order to analyze perlecan expression and neurogenesis in the cerebral cortex. We showed that the thickness of cerebral cortex, the number of cells produced in the cerebral cortex and perlecan expression are increased in response to injection of FGF-2 and decreased in response to infusion of anti-FGF-2 antibody as compared to controls. In conclusion, the results from this study suggest that FGF-2 increases neural cell production and perlecan expression in the developing cerebral cortex. It is also concluded that FGF-2 is an important factor in the cerebral cortical development, stimulating neuronal precursor proliferation.

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Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells

Cited by 32 publications

References 27 publications

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

Early Differentiating Mouse Astroglial Progenitors Share Common Protein Signatures with GL261 Glioma Cells

Induction of Perlecan Expression and Neural Cell Proliferation by FGF-2 in the Developing Cerebral Cortex: An In Vivo Study

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