2006
DOI: 10.1590/s0001-37652006000300008
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Abstract: Toxoplasma gondii multiplies within parasitophorous vacuole that is not recognized by the primary no oxidative defense of host cells, mainly represented by the fusion with acidic organelles. Recent studies have already shown that hydroxyurea arrested the intracellular parasites leading to its destruction. In the present work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasma gondii. Fluorescent vital stains were used in order to observe possible acidification of para… Show more

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Cited by 7 publications
(8 citation statements)
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References 23 publications
(10 reference statements)
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“…The AO-positive vacuoles contained disorganized parasites ( Figure 7D), whereas the untreated culture con- tained parasites forming rosette structures. The acidification is probably caused by lysosome fusion, as demonstrated after HU treatment (25).…”
Section: Resultsmentioning
confidence: 92%
“…The AO-positive vacuoles contained disorganized parasites ( Figure 7D), whereas the untreated culture con- tained parasites forming rosette structures. The acidification is probably caused by lysosome fusion, as demonstrated after HU treatment (25).…”
Section: Resultsmentioning
confidence: 92%
“…In this context we may consider that infected cells recovered from infection following treatment with Mon. Connected with this, Melo et al [28] demonstrated that interruption of parasite cell multiplication using hydroxyurea also led to tachyzoite elimination, and as shown by Melo and Carvalho [29], this elimination was associated with lysosome-parasitophorous vacuole fusion, resulting in complete digestion of the parasites. In the present study, we did not perform assays to verify whether the PVs containing parasites after treatment with anti-Golgi agents also interact with the cell digestive pathway; however it is important to consider this possibility.…”
Section: Discussionmentioning
confidence: 91%
“…The disorganisation of the Golgi complex and any secretory organelles interrupts the survival of T. gondii in the cellular medium (Carvalho et al, 2009). Other authors have also demonstrated that the interruption of secretory organelles has antiparasitic effects (Udenya, 2004;Totino et al, 2008;Sturm et al, 2009).…”
Section: Discussionmentioning
confidence: 97%
“…There are over 50 different bioactive compounds (terpenoids and others) of these aqueous extracts, but the major components of neem and cinnamon are limonoids such as azadirachtins (AZ) (Huang et al, 1996;Nakatani et al, 1998;Kaushik, 2002;D'Ambrosio & Guerriero, 2002). The general biological action of these extracts is the induction of lipid peroxidation, generation of antiproliferative and antioxidant effects and detoxication of enzymes (Akudugu et al, 2001;Kumar et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
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