Detection of viral particles in feces of young dogs and their relationship with clinical signs

Amo, Alicia Noemí del; Aprea, Adriana Noemí; Petruccelli, Miguel Ángel

doi:10.1590/s0001-37141999000300009

Cited by 5 publications

(2 citation statements)

References 5 publications

(6 reference statements)

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“…Furthermore, in this study, the virus was detected through an immunochromatographic test of fecal samples from a symptomatic dog, confirmed by PCR, and then isolated in a CRFK cell line, as had also been previously described in other studies [9,24]. In addition to confirmation by PCR, the isolation of CPV-2 was confirmed by TEM, which revealed particles compatible with the parvovirus morphology, i.e., full and empty virus-like particles with polygonal outlines and diameters ranging from 19 to 28 nm [25,26]. Lastly, a virus hemagglutination test of the third passage exhibited a titer of 320.…”

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confidence: 60%

Isolation and genome characterization of canine parvovirus type 2c in Brazil

et al. 2018

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mentioning

confidence: 60%

Isolation and genome characterization of canine parvovirus type 2c in Brazil

et al. 2018

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“…The sediment was suspended in PBS at a 1:1 ratio. A negative contrast electron microscope was done according to (Amo et al, 1999) with some modification. One drop of homogenate was placed on a cooper grid then Grids were negatively stained with phosphotungstic acid, pH 7, 2% for 1 minute.…”

Section: Fecal Sample Preparation and Concentrationmentioning

confidence: 99%

Feline Pan Leukopenia Molecular Detection and Viral Phylogeny in Egypt

Khattab¹,

Yanni²,

Abdelmegeed³

et al. 2023

AAVS

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Feline panleukopenia virus (FPV) causes severe disease in cats, as well as in a variety of wild carnivores including many endangered animals. Regular appearance of the virus increases the importance of evaluating the percentage of identity in relation to vaccine commercially used, detecting sequence changes related to virus virulence or tropism. In this study Fifty-seven fecal samples were collected from suspected cases of cats. 19 fecal samples were positive using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the same animals were subjected for hematological examination to investigate the severity of leukopenia. Thirteen out of nineteen samples were positive with a polymerase chain reaction assay (PCR) targeting VP2 gene. Three positive fecal samples in animal showing sever and moderate leukopenia were successfully sequenced Partially. Sequence analysis results showed no variation in their sequence across all isolates when compared to the published FPV genome, which could imply that FPV appears to be in genomic stasis as compared to other Parvoviruses. Genomic stasis appears among the three Egyptian samples in this study under the GenBank accession number OP594200, OP574201, and OP574202, in addition to the high similarity of the isolates the vaccine strains of EU498680 and EU498681.No amino acid substitution was detected.

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Validation of a fecal scoring scale in puppies during the weaning period

Grellet

Feugier²,

Chastant-Maillard

et al. 2012

Preventive Veterinary Medicine

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In puppies weaning is a high risk period. Fecal changes are frequent and can be signs of infection by digestive pathogens (bacteria, viruses, parasites) and indicators of nutritional and environmental stress. The aim of this study was to define a pathological fecal score for weaning puppies, and to study the impact on that score of two intestinal viruses (canine parvovirus type 2 and canine coronavirus). For this, the quality of stools was evaluated on 154 puppies between 4 and 8 weeks of age (100 from small breeds and 54 from large breeds). The scoring was performed immediately after a spontaneous defecation based on a 13-point scale (from 1; liquid to 13; dry and hard feces). Fecal samples were frozen for further viral analysis. Each puppy was weighed once a week during the study period. The fecal score regarded as pathological was the highest score associated with a significant reduction in average daily gain (ADG). Fecal samples were checked by semi-quantitative PCR or RT-PCR for canine parvovirus type 2 and canine coronavirus identification, respectively. The quality of feces was affected by both age and breed size. In small breeds, the ADG was significantly reduced under a fecal score of 6 and 7 for puppies at 4-5 and 6-8 weeks of age, respectively. In large breeds, the ADG was significantly reduced under a fecal score of 5 whatever the age of the puppy. Whereas a high viral load of canine parvovirus type 2 significantly impacted feces quality, no effect was recorded for canine coronavirus. This study provides an objective threshold for evaluation of fecal quality in weaning puppies. It also emphasizes the importance to be given to age and breed size in that evaluation.

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Detection of viral particles in feces of young dogs and their relationship with clinical signs

Cited by 5 publications

References 5 publications

Isolation and genome characterization of canine parvovirus type 2c in Brazil

Isolation and genome characterization of canine parvovirus type 2c in Brazil

Feline Pan Leukopenia Molecular Detection and Viral Phylogeny in Egypt

Validation of a fecal scoring scale in puppies during the weaning period

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