Reconstruction of parietal bone defects with adiposederived mesenchymal stem cells. Experimental study

Silva, Diego Dias da; Paz, Ana Helena da Rosa; Portinho, Ciro Paz; Lima, Elizabeth Obino Cirne; Kliemann, Lúcia Maria; Collares, Marcus Vinícius Martins

doi:10.1590/acb351201

Cited by 6 publications

(4 citation statements)

References 36 publications

(51 reference statements)

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“…However, for osteogenic differentiation to be achieved, different growth factors or biomaterials should be combined to provide an osteogenic induction environment for ADSCs. The combination of ADSCs and autologous platelet-rich plasma (PRP) was not statistically significant compared with autologous bone grafts ( 161 ). This may be because the growth factor mixture in PRP has a short half-life in vivo , which cannot guarantee osteogenic induction of ADSCs during the whole process of bone repair.…”

Section: Implications For Treatmentmentioning

confidence: 99%

Impact of High-Altitude Hypoxia on Bone Defect Repair: A Review of Molecular Mechanisms and Therapeutic Implications

Chen

Liu

et al. 2022

Front. Med.

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Reaching areas at altitudes over 2,500–3,000 m above sea level has become increasingly common due to commerce, military deployment, tourism, and entertainment. The high-altitude environment exerts systemic effects on humans that represent a series of compensatory reactions and affects the activity of bone cells. Cellular structures closely related to oxygen-sensing produce corresponding functional changes, resulting in decreased tissue vascularization, declined repair ability of bone defects, and longer healing time. This review focuses on the impact of high-altitude hypoxia on bone defect repair and discusses the possible mechanisms related to ion channels, reactive oxygen species production, mitochondrial function, autophagy, and epigenetics. Based on the key pathogenic mechanisms, potential therapeutic strategies have also been suggested. This review contributes novel insights into the mechanisms of abnormal bone defect repair in hypoxic environments, along with therapeutic applications. We aim to provide a foundation for future targeted, personalized, and precise bone regeneration therapies according to the adaptation of patients to high altitudes.

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Section: Implications For Treatmentmentioning

confidence: 99%

Impact of High-Altitude Hypoxia on Bone Defect Repair: A Review of Molecular Mechanisms and Therapeutic Implications

Chen

Liu

et al. 2022

Front. Med.

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“…Da Silva et al [53] studied the potential regenerative role of autologous ADSCs with osteogenic differentiation embedded in a PRP scaffold, with or without the administration of papaverine (PPV), in rats with surgical parietal defect versus autologous parietal bone graft transplantation. The rats were euthanatized after 28 days.…”

Section: Bonementioning

confidence: 99%

The Combined Use of Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem Cells Promotes Healing. A Review of Experimental Models and Future Perspectives

et al. 2021

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Wound healing and tissue regeneration are a field of clinical medicine presenting high research interest, since various local and systematic factors can inhibit these processes and lead to an inferior result. New methods of healing enhancement constantly arise, which, however, require experimental validation before their establishment in everyday practice. Platelet-rich plasma (PRP) is a well-known autologous factor that promotes tissue healing in various surgical defects. PRP derives from the centrifugation of peripheral blood and has a high concentration of growth factors that promote healing. Recently, the use of adipose-derived mesenchymal stem cells (ADMSCs) has been thoroughly investigated as a form of wound healing enhancement. ADMSCs are autologous stem cells deriving from fat tissue, with a capability of differentiation in specific cells, depending on the micro-environment that they are exposed to. The aim of the present comprehensive review is to record the experimental studies that have been published and investigate the synergistic use of PRP and ADMSC in animal models. The technical aspects of experimentations, as well as the major results of each study, are discussed. In addition, the limited clinical studies including humans are also reported. Future perspectives are discussed, along with the limitations of current studies on the long-term follow up needed on efficacy and safety.

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“…MSCs are a heterogeneous mesenchymal stem cell subpopulation with the potential for multidirectional differentiation into mesoderm, ectoderm and endoderm lineages ( Bianco and Gehron Robey, 2000 ). Therefore, stem cell therapy is considered to be a potential effective strategy to regenerate bone tissues ( Silva et al, 2021 ). However, stem cell therapy also has some disadvantages that restrict its wider clinical applications, including phenotypic changes, immune rejection, potential tumorigenicity, low homing efficiency and cell survival rate ( Sissung and Figg, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The potential therapeutic role of extracellular vesicles in critical-size bone defects: Spring of cell-free regenerative medicine is coming

Liu

Sun²,

An³

et al. 2023

Front. Bioeng. Biotechnol.

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In recent years, the incidence of critical-size bone defects has significantly increased. Critical-size bone defects seriously affect patients’ motor functions and quality of life and increase the need for additional clinical treatments. Bone tissue engineering (BTE) has made great progress in repairing critical-size bone defects. As one of the main components of bone tissue engineering, stem cell-based therapy is considered a potential effective strategy to regenerate bone tissues. However, there are some disadvantages including phenotypic changes, immune rejection, potential tumorigenicity, low homing efficiency and cell survival rate that restrict its wider clinical applications. Evidence has shown that the positive biological effects of stem cells on tissue repair are largely mediated through paracrine action by nanostructured extracellular vesicles (EVs), which may overcome the limitations of traditional stem cell-based treatments. In addition to stem cell-derived extracellular vesicles, the potential therapeutic roles of nonstem cell-derived extracellular vesicles in critical-size bone defect repair have also attracted attention from scholars in recent years. Currently, the development of extracellular vesicles-mediated cell-free regenerative medicine is still in the preliminary stage, and the specific mechanisms remain elusive. Herein, the authors first review the research progress and possible mechanisms of extracellular vesicles combined with bone tissue engineering scaffolds to promote bone regeneration via bioactive molecules. Engineering modified extracellular vesicles is an emerging component of bone tissue engineering and its main progression and clinical applications will be discussed. Finally, future perspectives and challenges of developing extracellular vesicle-based regenerative medicine will be given. This review may provide a theoretical basis for the future development of extracellular vesicle-based biomedicine and provide clinical references for promoting the repair of critical-size bone defects.

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Reconstruction of parietal bone defects with adiposederived mesenchymal stem cells. Experimental study

Abstract: Design of the study, analysis of data, technical procedures, manuscript writing.

Cited by 6 publications

References 36 publications

Impact of High-Altitude Hypoxia on Bone Defect Repair: A Review of Molecular Mechanisms and Therapeutic Implications

Impact of High-Altitude Hypoxia on Bone Defect Repair: A Review of Molecular Mechanisms and Therapeutic Implications

The Combined Use of Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem Cells Promotes Healing. A Review of Experimental Models and Future Perspectives

The potential therapeutic role of extracellular vesicles in critical-size bone defects: Spring of cell-free regenerative medicine is coming

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