In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Silva, Daniel da; Imakawa, Angela Maria; Costa, Suely de Souza; Sampaio, Paulo de Tarso Barbosa

doi:10.1590/2236-8906-65/2018

Cited by 3 publications

(2 citation statements)

References 20 publications

(17 reference statements)

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“…It appears that the medium without phytohormones is the most favourable for rooting with 100% rooted explants demonstrating that the rooting of P. nitida embryos does not require an additional supply of phytohormones (Table 3). These results are similar with the development of Caesalpinia ferrea zygotic embryos (Da Silva et al 2018), where the control medium had the highest rooting rate. They also found that exogenous phytohormones…”

Section: Resultssupporting

confidence: 84%

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Kamdem

Donfagsiteli²,

Mache

et al. 2022

Plant Tissue Cult. & Biotech.

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Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida. Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)

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Section: Resultssupporting

confidence: 84%

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Kamdem

Donfagsiteli²,

Mache

et al. 2022

Plant Tissue Cult. & Biotech.

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“…Zygotic embryo explant is superior to other regeneration systems due to its independent genotype. The zygotic embryo has been accepted as an adequate explant for tissue culture and genetic transformation in many medicinal plants [ 32 – 35 ]. Since, there have been difficulties with the regeneration of plants from Agrobacterium -infected zygotic embryo, the Trachyspermum ammi transformation system is established.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

Nomani

Tohidfar

2021

Journal of Genetic Engineering and Biotechnology

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Background Trachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with high anti-microbial, anti-bacterial, and anti-oxidant properties. Thymol in the latest research has a significant impact on slowing the progression of cancer cells in human. In this research, embryos were employed as convenient explants for the fast and effectual regeneration and transformation of T. ammi. To regenerate this plant, Murashige and Skoog (MS) and Gamborg's B5 (B5) media were supplemented with diverse concentrations of plant growth regulators, such as 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (kin). Transgenic Trachyspermum ammi plants were also obtained using Agrobacterium-mediated transformation and zygotic embryos explants. Moreover, two Agrobacterium tumefaciens strains (EHA101 and LBA4404) harboring pBI121-TPS2 were utilized for genetic transformation to Trachyspermum ammi. Results According to the obtained results, the highest plant-regeneration frequency was obtained with B5 medium supplemented with 0.5 mg/l BA and 1 mg/l NAA. The integrated gene was also approved using the PCR reaction and the Southern blot method. Results also showed that the EHA101 strain outperformed another strain in inoculation time (30 s) and co-cultivation period (1 day) (transformation efficiency 19.29%). Furthermore, HPLC method demonstrated that the transformed plants contained a higher thymol level than non-transformed plants. Conclusions In this research, a fast protocol was introduced for the regeneration and transformation of Trachyspermum ammi, using zygotic embryo explants in 25–35 days. Our findings confirmed the increase in the thymol in the aerial part of Trachyspermum ammi. We further presented an efficacious technique for enhancing thymol content in Trachyspermum ammi using Agrobacterium-mediated plant transformation system that can be beneficial in genetic transformation and other plant biotechnology techniques.

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Doğu Mazısının (Thuja orientalis L.) Embriyo Kültürü İle Çoğaltımı

Sezgin

Kahya

2021

Türk Tarım Ve Doğa Bilimleri Dergisi

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Cupresseaceae familyasına ait Doğu mazısı (Thuja orientalis L.), peyzaj açısından kullanımıyla birlikte kereste ve odun üretimi için önemli bir orman ağacı türüdür. Bununla birlikte tıbbi olarak kullanımı mazıyı oldukça önemli bitkiler sınıfına sokmaktadır. Çalışmada, Doğu mazısı (Thuja orientalis L.) bitki biyoteknolojisinin önemli tekniklerinden biri olan embriyo kültürü tekniği ile çoğaltarak oldukça önemli sonuçlar elde edilmiştir. Sakkarozun farklı dozları ile birlikte, Woody Plant Medium (WPM) temel besin ortamına 6-benziladenin (BA)'in (0, 1, ve 2 mg l -1 ) ile 2,4-Diklorofenoksi asetik asit (2,4-D) veya Indol-3-bütirik asit (IBA)'in (0, 0.5 ve 1 mg l -1 ) dozları kombine edilerek besin ortamına ilave edilmiştir. BA (1 mg l -1 ) + IBA (0.5 mg l -1 ) büyüme düzenleyici madde (BDM) kombinasyonuna 30 g l -1 sakkaroz ilave edilmiş ortamda çimlenme başarısı %100 oranında meydana gelmiş, maksimum sürgün uzunluğu (4.8 cm) ve kök sayısı (7.6) elde edilmiştir. BA (1 mg l -1 ) + 2,4-D (0.5 mg l -1) BDM kombinasyonuna 45 g l -1 sakaroz eklenmiş ortamda ise maksimum kök uzunluğu 2.4 cm olarak belirlenmiştir. Aklimatizasyon uygulaması yapılan bitkilerden %69'u dış koşulara uyum göstermiştir.

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In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Cited by 3 publications

References 20 publications

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

Doğu Mazısının (Thuja orientalis L.) Embriyo Kültürü İle Çoğaltımı

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