2016
DOI: 10.1590/1984-70332016v16n3n35
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Abstract: This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo

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Cited by 11 publications
(9 citation statements)
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“…Genotyping to estimate genetic diversity among the lines used 24 microsatellite markers grouped into four multiplex panels containing six fluorescent markers each (Cardoso et al, 2013; Morais et al, 2016). The products of DNA amplification were analyzed in the automatic sequencer ABI 3500 XL (Applied Biosystems) and genotyping was performed by the computational program GeneMapper version 4.1 AB (Applied Biosystems).…”
Section: Methodsmentioning
confidence: 99%
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“…Genotyping to estimate genetic diversity among the lines used 24 microsatellite markers grouped into four multiplex panels containing six fluorescent markers each (Cardoso et al, 2013; Morais et al, 2016). The products of DNA amplification were analyzed in the automatic sequencer ABI 3500 XL (Applied Biosystems) and genotyping was performed by the computational program GeneMapper version 4.1 AB (Applied Biosystems).…”
Section: Methodsmentioning
confidence: 99%
“…The crosses were made under greenhouse condition within a water mist climate‐controlled system in Santo Antônio de Goiás, Goiás. The F 1 seeds from the crosses were sown under greenhouse conditions, and the hybrid nature of all the F 1 plants was confirmed using microsatellite markers (Morais et al, 2016).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Thus, the molecular information obtained in this study were a complementary criterion to direct selection of individuals for the next recombination cycle. The contribution and relevance of the use of molecular markers in breeding of common bean has been described by various authors, with emphasis on gains from selection for traits related to both biotic and abiotic stresses (Yu et al, 2000, Miklas et al, 2006Morais et al, 2016;Oliveira et al, 2008).…”
Section: Discussionmentioning
confidence: 99%
“…Twenty-four fluorescent microsatellite markers were previously chosen based on their high informative content, distributed in four multiplex panels containing six markers each (Morais et al, 2016). DNA was extracted from bulk samples obtained from leaves of 10 plants from each genotype using the alkaline extraction method, as described by Xin et al (2003).…”
Section: Crosses Plant Materials and Phenotypic Screeningmentioning
confidence: 99%