2020
DOI: 10.1590/1984-3143-ar2020-0055
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Abstract: How to cite: Gu B. Light up the embryos: knock-in reporter generation for mouse developmental biology.

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Cited by 3 publications
(3 citation statements)
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References 38 publications
(39 reference statements)
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“…Some studies have relied on a 2-cell like state or "2C" state in ESCs to model embryonic EGA. Activating factors include DPPA2, DPPA3/STELLA, DPPA4, DUX, ZSCAN4, and OBOX 4 (Alda-Catalinas et al, 2020;Amano et al, 2013;De Iaco et al, 2017, 2019, 2020Eckersley-Maslin et al, 2019;Falco et al, 2007;Guo et al, 2022;Hendrickson et al, 2017;Huang et al, 2017Huang et al, , 2021Ko, 2016;Li et al, 2021;Ruebel et al, 2019;Yan et al, 2019;Zhang, Wen, et al, 2022) (Figure 6). A loose regulatory hierarchy apparently exists wherein DPPA2 and DPPA4 activate Dux, and DUX then activates Zscan4 and other EGA1 genes.…”
Section: Somatic Cell Nuclear Transfer As a Window Onto Early Embryon...mentioning
confidence: 99%
See 1 more Smart Citation
“…Some studies have relied on a 2-cell like state or "2C" state in ESCs to model embryonic EGA. Activating factors include DPPA2, DPPA3/STELLA, DPPA4, DUX, ZSCAN4, and OBOX 4 (Alda-Catalinas et al, 2020;Amano et al, 2013;De Iaco et al, 2017, 2019, 2020Eckersley-Maslin et al, 2019;Falco et al, 2007;Guo et al, 2022;Hendrickson et al, 2017;Huang et al, 2017Huang et al, , 2021Ko, 2016;Li et al, 2021;Ruebel et al, 2019;Yan et al, 2019;Zhang, Wen, et al, 2022) (Figure 6). A loose regulatory hierarchy apparently exists wherein DPPA2 and DPPA4 activate Dux, and DUX then activates Zscan4 and other EGA1 genes.…”
Section: Somatic Cell Nuclear Transfer As a Window Onto Early Embryon...mentioning
confidence: 99%
“…It is likely that a detailed understanding of the dynamic regulation of oocyte and preimplantation proteomes and functions of individual proteins will be facilitated through a combination of genetic modifications to allow tagging of proteins for in situ detection, spatial imaging, and monitoring, and to allow experimentally induced degradation and replenishment of proteins in a controlled manner. Recent technologies allowing for insertion of tags through homology‐mediated DNA editing provide such opportunities (Abuhashem & Hadjantonakis, 2021; Gu, 2020).…”
Section: How Are Maternal Proteins Stored and Regulated?mentioning
confidence: 99%
“…Besides studies on the cochlea and inner ear, the lightsheet microscope has also been used to record entire embryonic development processes (Andilla et al, 2017;Bardot & Hadjantonakis, 2020;Keller, 2013;Nowotschin, Garg, Piliszek, & Hadjantonakis, 2019;Sutherland, 2016) and both preimplanation (Reichmann, Eguren, Lin, Schneider, & Ellenberg, 2018;Strnad et al, 2016) and postimplantation (McDole et al, 2018;Udan, Piazza, Hsu, Hadjantonakis, & Dickinson, 2014;Wan et al, 2019) studies of mouse embryo development for which knock-in reporters are now becoming available (Gu, 2020;Li, Chen, et al, 2018). Ichikawa et al (2014) and Candeo et al (2016) have respectively published protocols for studying gastrulation and the mouse intestine with the lightsheet microscope.…”
Section: Background Informationmentioning
confidence: 99%