Effect of COQ9 and STAT5A polymorphisms on reproductive performance in a Holstein cow herd in Mexico

Abstract

Coenzyme Q9 (COQ9), a coenzyme Q (CoQ) precursor, is an essential component of the mitochondrial electron transport chain that drives adenosine triphosphate production. COQ9 polymorphism 18:25527339 is characterized by substitution of guanine (allele G) for adenine (allele A), which modifies the function of the protein encoded by the gene. In Holsteins, allele A has been associated with better reproductive performance in terms of the conception rate, number of services per conception (SPC) and days open (DO). The signal transducer and activator of transcription (STAT) protein is a transcription factor activated in the presence of cytokines and growth factors. STAT5A polymorphism 19:42407732 in exon 8 has been associated with higher fertility and embryonic survival rates. The objective of this study was to determine the relationship of COQ9 and STAT5A polymorphisms with reproductive parameters [calving to first heat interval (CFHI), DO and SPC]. Blood samples were taken from 112 lactating Holstein from a herd in México for allele genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). To estimate the association between reproductive parameters and genotypes, a linear mixed-effect model was performed. The COQ9 AG genotype was associated significantly with lower SPC (P<0.05) but not with DO or CFHI. No significant association with any reproductive parameter was found for STAT5A. Our findings suggest that the COQ9 18:25527339 polymorphism is a useful molecular marker for improvement of reproductive performance in dairy herds.

Association between somatotropic axis gene polymorphisms and reproductive efficiency of bovine females: a review

“…Michel-Regalado et al (33) SNP12195 74 -Maintained alleles showed a high correlation with the decrease in CI (r = 0.94, p < 0.05) (Table 6). The N°services/pregnancy was described by Bergamaschi et al (42) as the number of matings or AI divided by the total number of cows that became pregnant.…”

“…Given the knowledge between associations with the polymorphic variants of genes involved in reproduction and phenotypic characteristics, it is possible to use selection methods and techniques involving molecular markers for improved animal productivity and efficiency (44) . The frequencies of alleles and genotypes studied and used as a basis for this review (32,27,31,23,30,28,24,26,29,33) are available for consultation in the supplementary material.…”

Associação entre polimorfismos de genes do eixo somatotrópico e eficiência reprodutiva de fêmeas bovinas: revisão de literatura

“…Michel-Regalado et al (33) SNP12195 74 -Manteve A transição T/C localizada na região promotora do gene IGF-I pode sugestionar a concentração sérica de IGF-I em vacas holandesas do Irã. Até então, a variante relatada nessa população de gado leiteiro do Irã não é funcional, é provável que a baixa associação do SNP, dada pelo valor significância, com essas características seja porque ocorre um desequilíbrio de ligação com outras variantes no IGF-I ou loci de traços quantitativos (QTL) que são relatados nessa localidade (30) (Tabela 5).…”

“…Diante do conhecimento entre associações com as variantes polimórficas dos genes envolvidos na reprodução e das características fenotípicas, é possível o uso de métodos e técnicas de seleção com uso de marcadores moleculares, para melhor aproveitamento da produtividade e eficiência animal (44) . A frequência dos alelos e genótipos estudados nos trabalhos citados e usados como base nesta revisão (32,27,31,23,30,28,24,26,29,33) estão disponíveis para consulta no material suplementar.…”

“…Hax et al (25) STAT5A/BstEII 2,16 Aumentou Hax et al (25) STAT5A/BstEII 2,24 Aumentou Hax et al (25) STAT5A/BstEII 2,18 Aumentou Hax et al (25) STAT5A/BstEII 2,7 Aumentou Hax et al (25) STAT5A/BstEII 2,8 Aumentou Hax et al (25) STAT5A/BstEII 2,4 Aumentou Silveira et al (29) STAT5A/BstEII 2,6 Aumentou Silveira et al (29) STAT5A/BstEII 2,6 Aumentou Silveira et al (29) STAT5A/BstEII 2,5 Aumentou Oikonomou et al (32) SNP12195 0 -Oikonomou et al (32) SNP12195 0 -Oikonomou et al (32) SNP12195 0 -Michel-Regalado et al (33) SNP12195 3,1 Aumentou…”