Dimethylacetamide alone or in combination with glycerol can be used for cryopreservation of ovine semen

Menezes, Gabriel Felipe Oliveira de; Bittencourt, Rodrigo Freitas; Cardoso, Fernando de Lima; Lents, Maicon Pereira; Santos, E. S.; Barreto, Renata Oliveira; Jesus, Edivânia Oliveira de; Madrigal‐Valverde, Mónica; Filho, Antônio de Lisboa Ribeiro

doi:10.1590/1984-3143-ar2020-0036

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…The addition of substances for semen cryopreservation to maintain its viability and quality has been evidenced, bringing benefits to the freezing ( 34 , 35 ) or cooling of semen ( 36 ). And as a dietary strategy, the supplementation with palm kernel cake does not affect seminal parameters and thus could be used as an alternative ingredient in sheep diets ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review

2022

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This study identifies the number of publications that presented technologies used in the production systems of Santa Inês sheep in the last 5 years (2017–2021) carried out in Brazil. Therefore, the objective was to identify where we are in terms of knowledge about technologies in different fields (health, reproduction, animal breeding, behavior and welfare, nutrition and feeding, forage and pasture, carcass and meat quality, and economics and management of livestock systems). After rigorous selection, 114 studies were appointed and classified by knowledge field, and the main approaches within each theme were evaluated, pointing out research gaps. Most technologies have been in northeastern states. However, government agencies should develop public policies to disseminate techniques in rural areas because the production system in this region is still subsistence. This study highlighted the need for works that present management practices and tools that impact the improvement of animal welfare. Agro-industrial by-products have been widely used as an alternative for sheep feeding. However, economic feasibility analyses are recommended with these foodstuffs to substantiate their use as an option to reduce production costs. There is a lack of research allusive to the management of production systems, especially those related to estimates of economic feasibility indicators.

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Section: Discussionmentioning

confidence: 99%

Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review

2022

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“…Structural difference exists in the plasma membrane of the sperm among the species (Ladha, 1998). CPA with different chemical groups has different permeability coefficient in different species (Menezes et al, 2021). Kim et al(2011) observed lower acrosome integrity with 3 per cent DMA compared to glycerol.…”

Section: Functional Membrane Integrity and Acrosome Integritymentioning

confidence: 93%

Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen

Kanimozhi¹,

Chacko²,

Promod³

et al. 2023

Journal of Veterinary and Animal Sciences

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The key role of a cryoprotectant is to minimize the chemical and physical stress which occurs during cooling, freezing and thawing of semen. The difference between the cryoprotectant (CPA) occurs in their permeability coefficient and the structural model of the cryogenic agent. The beneficial effect of dimethylacetamide (DMA) as a cryoprotectant especially for sperms had been observed in several studies. The aim of the study was to study the cryoprotective effect of DMA in freezing the Malabari buck semen compared to glycerol. Ten ejaculates were taken from fourMalabaribucks . After preliminary evaluation sample split technique was followed with Tris based extender containing glycerol (6.7 per cent) as cryoprotectant (control) and Trisextender containing DMA (3 per cent) as cryoprotectant (treatment group). The semen straws (0.25mL) after filling were subjected for equilibration and manual freezing. Sperm kinetics was studied using computer-aided sperm analyzer. Pre-freeze and post-thaw evaluation included sperm viability, sperm abnormality, hypo osmotic test, acrosome integrity test and DNA fragmentation. Results indicated that inclusion of 6.7 per cent glycerol had significantly higher (p<0.05) post-thaw values than DMA. From our study we conclude that 6.7 per cent glycerol was better than 3 per cent DMA in cryopreservation of Malabari buck semen.

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Advances in Cryopreservatives: Exploring Safer Alternatives

Lomba,

García,

Benito

et al. 2023

ACS Biomater. Sci. Eng.

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Cryopreservation of cells, tissues, and organs is widely used in the biomedical and research world. There are different cryopreservatives that are used for this process; however, many of them, such as DMSO, are used despite the problems they present, mainly due to the toxicity it presents to certain types of samples. The aim of this Review is to highlight the different types of substances used in the cryopreservation process. It has been shown that some of these substances are well-known, as in the case of the families of alcohols, sugars, sulfoxides, etc. However, in recent years, other compounds have appeared, such as ionic liquids, deep eutectic solvents, or certain polymers, which open the door to new cryopreservation methods and are also less toxic to frozen samples.

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Dimethylacetamide alone or in combination with glycerol can be used for cryopreservation of ovine semen

Cited by 3 publications

References 30 publications

Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review

Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review

Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen

Advances in Cryopreservatives: Exploring Safer Alternatives

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