Characterization of mesenchymal stem cells derived from adipose tissue of a cougar (Puma concolor)

Retinoic acid induces the osteogenic differentiation of cat adipose tissue‐derived stromal cells from distinct anatomical sites

“…In cats regenerative orthopedic procedures, MSCs or MSCs‐like populations have been isolated from various tissues such as bone marrow (Martin et al, 2002; Webb et al, 2011), adipose tissue (Echeverry et al, 2020; Fujimoto et al, 2020; Voga et al, 2021), and peripheral blood (Sato et al, 2016). Regarding these, adipose tissue is considered one of the most attractive sources as it yields a large number of cells, is widely available, and can be obtained by minimally invasive procedures without tissue damage or excessive scarring (Kern et al, 2006).…”

“…Broadly, it can be collected from two main sites—subcutaneous and visceral—with established differences regarding structure, composition, and cell content (Casteilla et al, 2011), that can expectedly give rise to differences in proliferation, differentiation potential, and functionality of isolated MSCs, influencing the regenerative outcomes (Cleal et al, 2017). Notwithstanding, the comparative analysis of cats adipose‐derived MSCs from the distinct biological sites has not been previously conducted, with available data focusing exclusively on the osteogenic differentiation capability of populations isolated from the subcutaneous region (Echeverry et al, 2020; Fujimoto et al, 2020; Voga et al, 2021). Previous studies, carried out on distinct animal species—namely dogs, horses, and rabbits, do report differences on the morphology and functionality of adipose‐derived MSCs, according to their anatomical location of origin (Arnhold et al, 2019; Peptan et al, 2006; Requicha et al, 2012; Yaneselli et al, 2018), substantiating the importance of this comparative analysis.…”

“…Briefly, adipose tissue‐derived stromal cells (ADSCs) were grown and differentiated into the osteogenic lineage through incubation with osteogenic differentiating factors. Classically, this is achieved by culture supplementation with the combination of dexamethasone, β‐glycerophosphate, and ascorbic acid (Echeverry et al, 2020; Kono et al, 2014; Martin et al, 2002). More recently, some studies suggested that retinoic acid (RA) may be regarded as a new osteogenic inducer (Levi et al, 2011; Song et al, 2005; Wan et al, 2006), being able to promote the in vitro osteogenic differentiation of precursor cell populations in the rat, mouse, and canine (Levi et al, 2011, Song et al, 2005, Wan et al, 2006), with undisclosed functionality on cat derived cell populations.…”

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

“…The development of oocyte cryopreservation is critical for the establishment of genetic banks as well as the development of applications for the conservation of endangered European mink. In addition, keeping MSC in biobanks is a good way to ensure the genetic diversity of many endangered species [ 18 , 45 , 50 ]. This emMSC biobank will also enable the development of in vitro models for toxicologic, epidemiologic, or infectious disease studies [ 51 , 52 , 53 ], which is a valuable tool for understanding the molecular mechanisms of potential animal health threats that can affect endangered species.…”

Ultrastructural and morphometric description of the ear skin and cartilage of two South American wild histricognate rodents (Dasyprocta leporina and Galea spixii)

“…These animals play different roles in the functionality of the environment that they inhabit and are models for rodents under the threat of extinction (Praxedes et al 2018b), since their populations are stable (Catzeflis et al 2016, Emmons & Reid 2016. In other species, the use of an experimental model with a stable population has already been and interesting alternative and an important tool used in animal conservation research programs (Cardoso et al 2020, Echeverry et al 2020.…”