Influence of long-term thermal stress on the in vitro maturation on embryo development and Heat Shock Protein abundance in zebu cattle

Pöhland, Ralf; Souza-Cácares, M.B.; Datta, Tirtha Kumar; Vanselow, Jens; Martins, Maria Isabel Mello; Silva, Wilian Aparecido Leite da; Cardoso, Christopher Junior Tavares; Sterza, Fabiana de Andrade Melo

doi:10.1590/1984-3143-ar2019-0085

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…For example, we showed that different tropical breeds [ 92 ] respond differently to the “stress” they are subjected to, especially under different housing conditions [ 93 ]. Additionally, the duration and intensity of in vitro thermal stress [ 94 ] exhibited an interesting pattern of oocyte and embryo development after maturation under cold and high temperatures. However, the biological processes involved are not yet completely understood, and we speculate that lipid metabolism can explain some of the open questions.…”

Section: Heat Stressmentioning

confidence: 99%

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

Sterza

Poehland

2021

IJMS

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Lipids are a potential reservoir of energy for initial embryonic development before activation of the embryonic genome and are involved in plasma membrane biosynthesis. Excessive lipid droplet formation is detrimental to cryotolerance and is related to alterations in mitochondrial function, which likely affects lipid metabolism. Increased lipid accumulation in in vitro produced embryos is a consequence of the stress during in vitro embryonic development process. There are several open questions concerning embryo lipid metabolism and developmental potential. Oocyte maturation and embryo development in vivo and in vitro may vary if the donors are subjected to any type of stress before follicle puncture because crucial changes in oocyte/embryonic metabolism occur in response to stress. However, little is known about lipid metabolism under additional stress (such as heat stress). Therefore, in this review, we aimed to update the information regarding the energy metabolism of oocytes and early bovine embryos exhibiting developmental competence, focusing on lipid metabolic pathways observed under in vivo, in vitro, and stress conditions.

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Section: Heat Stressmentioning

confidence: 99%

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

Sterza

Poehland

2021

IJMS

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“…During in vitro culture, early embryos are exposed to limited nutrient availability and furthermore, they are influenced by the end products of their metabolism [ 223 ]. A stress response can be induced through the manipulation of embryos by pipetting, exposure to thermal stress, and/or detrimental change in pH [ 224 , 225 ]. It is evident that embryo in vitro culture conditions contribute to its epigenetic status.…”

Section: Translation Of Epigenetics Into Artmentioning

confidence: 99%

An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective

2022

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Germ cell quality is a key prerequisite for successful fertilization and early embryo development. The quality is determined by the fine regulation of transcriptomic and proteomic profiles, which are prone to alteration by assisted reproduction technology (ART)-introduced in vitro methods. Gaining evidence shows the ART can influence preset epigenetic modifications within cultured oocytes or early embryos and affect their developmental competency. The aim of this review is to describe ART-determined epigenetic changes related to the oogenesis, early embryogenesis, and further in utero development. We confront the latest epigenetic, related epitranscriptomic, and translational regulation findings with the processes of meiotic maturation, fertilization, and early embryogenesis that impact the developmental competency and embryo quality. Post-ART embryo transfer, in utero implantation, and development (placentation, fetal development) are influenced by environmental and lifestyle factors. The review is emphasizing their epigenetic and ART contribution to fetal development. An epigenetic parallel among mouse, porcine, and bovine animal models and human ART is drawn to illustrate possible future mechanisms of infertility management as well as increase the awareness of the underlying mechanisms governing oocyte and embryo developmental complexity under ART conditions.

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“…In addition, in vivo experiments have reported reduced pregnancy rate by 25% for each °C elevation in body temperature, which is due to the negative impact of heat stress on preimplantation development embryos (Nabenishi et al, 2012). Recently, heat shock at 40°C for 24 h during IVM of bovine COCs had no effect on Pb extrusion rate however; it reduced the rate of embryo cleavage and blastocyst development (Pöhland et al, 2020).…”

Section: Effect Of Heat Stress On the Development Rate Of In Vitro Produced Camel Embryos From Oocytes With Different Qualitymentioning

confidence: 99%

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

Ashour¹,

El-Sayed²,

Khalifa³

et al. 2020

WVJ

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The deleterious effect of heat stress on cumulus-oocytes complexes (COCs) competence is well recognized in different livestock species. Therefore, the present study aimed to investigate the effect of physiologically relevant heat stress on the developmental competence of camel COCs during in vitro maturation (IVM). A total of 1548 COCs were divided into six groups in this study. The groups were named K1 and K2 representing good and low-quality COCs incubated at 38.5oC for 30 hours. While K3 and k4 represent good and low-quality COCs exposed to 41oC for the first 6 hours of IVM. Finally, K5 and k6 represent the groups of good and low-quality COCs exposed to 42oC for the first 6 hours of IVM. After exposure of COCs to heat stress at 41°C and 42°C during the first 6 hours of in vitro maturation, the COCs were incubated at 38.5°C for 24 hours of IVM. The in vitro matured COCs were activated to cleave using ethanol followed by 4 mM 6-DMAP and developed embryos were cultured in vitro for 7 days post parthenogenetic activation. The results of this study indicated that heat stress at 42oC significantly decreased the Pb (polar body) extrusion rate in K4 and K6, compared to other groups. Additionally, the embryo cleavage rate was significantly lower for good and low-quality oocytes exposed to heat stress (K2, K3, K4, K5, and K6), compared to good quality COCs of the control group (K1). The cleavage rate was lower for low quality (K2; 63 ± 1.28) than good quality COCs (K1; 53 ± 1.85). The percentages of oocytes that developed to the blastocyst stage were lower for K2, K3, K4, K5, and K6 than K1. Moreover, the blastocyst rate was lower for K2 (9 ± 0.22) than K1 (15 ± 0.22). The results of this study indicated that exposure of camel oocytes to heat stress for 6 hours during in vitro maturation severely reduced extrusion of polar body, cleavage, and blastocyst rates. The low-quality camel COCs were reduced developmental capacity than good quality oocytes.

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Influence of long-term thermal stress on the in vitro maturation on embryo development and Heat Shock Protein abundance in zebu cattle

Cited by 7 publications

References 45 publications

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

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