2014
DOI: 10.1590/1983-084x/13_005
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Cultivo in vitro de Brosimum gaudichaudii Tréc. (Moraceae)

Abstract: RESUMO:O objetivo desta pesquisa foi avaliar o potencial organogenético de entrenós, discos foliares, e raízes de Brosimum gaudichaudii utilizando 12 diferentes combinações dos fitorreguladores 6-benzilaminopurina e ácido naftaleno acético, em meio MS (diluído à metade), sólido (6,5 g de ágar), e com 20 g.L -1 de sacarose. Independentemente das combinações hormonais testadas verificou-se a formação de calos friáveis (2 a 20 mm de diâmetro) em 90% dos entrenós usados como explantes. No entanto, os tratamentos t… Show more

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Cited by 4 publications
(5 citation statements)
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References 12 publications
(6 reference statements)
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“…Chlorine-based solutions, such as the sodium hypochlorite used in the present study, are frequently used, and are effective for using in the superficial cleaning of explants of several forest species (OLIVEIRA; DIAS; BRONDANI, 2013). Glutaraldehyde, on the other hand, although it has a biocidal function with a wide spectrum of action against bacteria, fungi, and viruses (TIPPLE et al, 2004), is rarely used in disinfestation treatments of explants for introduction in vitro, and is commonly used to fix cellular structures in vegetal anatomical analysis (LIMA et al, 2014). Although little used in surface disinfestation of explants, our objective was to evaluate its biocidal potential in the search for efficient treatments for the elimination of fungi and bacteria for use in the culture of plant tissues.…”
Section: Resultsmentioning
confidence: 99%
“…Chlorine-based solutions, such as the sodium hypochlorite used in the present study, are frequently used, and are effective for using in the superficial cleaning of explants of several forest species (OLIVEIRA; DIAS; BRONDANI, 2013). Glutaraldehyde, on the other hand, although it has a biocidal function with a wide spectrum of action against bacteria, fungi, and viruses (TIPPLE et al, 2004), is rarely used in disinfestation treatments of explants for introduction in vitro, and is commonly used to fix cellular structures in vegetal anatomical analysis (LIMA et al, 2014). Although little used in surface disinfestation of explants, our objective was to evaluate its biocidal potential in the search for efficient treatments for the elimination of fungi and bacteria for use in the culture of plant tissues.…”
Section: Resultsmentioning
confidence: 99%
“…Assim, a atividade antioxidante da mama-cadela foi analisada em um estudo realizado por Coutinho et al (2019), no qual foi demostrado que sua atividade antioxidante é de 26,46%, a mesma análise demonstrada por Land et al (2017) resultou no valor de 28,85 ± 2,66. Lima et al (2014) relataram a presença de efeitos antioxidantes na casca da raiz e do caule da mamacadela e devido essa atividade ela vem sendo utilizada no tratamento do vitiligo. Por isso, a pesquisa empreendida propôs-se a realização da análise do teor de fenóis totais e a avaliação da atividade antioxidante da espécie Brosimum gaudichaudii para avaliar sua possível eficiência no combate ao estresse oxidativo, a qual demonstrou um valor considerável indicando que a espécie tem atuação importante contra os processos oxidativos que desencadeiam o vitiligo, podendo ser uma importante aliada em seu tratamento.…”
Section: Mama-cadelaunclassified
“…The Cerrado is a biome with a wide geographical occurrence; hence B. gaudichaudii shows regional variations in seed germination: Faria et al [11,12] observed that seeds from the Mato Grosso state display germinability higher than 80% after the removal of the seed coat. However, the seeds collected in Goias state germinate after simply being embedded in water, without needing any scarification [13,14]. Evidences exist that the seeds and seedlings largely vary in quality and vigor according to the precedence, reflecting the genetic variability of this wild plant [11].…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, [18] documented the production of B. gaudichaudii saplings from root cuttings with a success rate of about 30% independently from a treatment with phytoregulators. Both Lima et al [13] and Silva et al [18] reported difficulties in obtaining saplings from shoot/branch cuttings.…”
Section: Introductionmentioning
confidence: 99%