Influência de diferentes extratos aquosos de plantas medicinais no desenvolvimento de Colletotrichum gloeosporioides e de Fusarium moniliforme

Abstract

5, 10 and 20% (w:v) and incorporated into the PDA (potato dextrose agar) culture medium before sterilization by autoclaving. Later, an 8 mm diameter disc of each pathogen mycelium was transferred to the center of the Petri dishes. After 24, 48 and 96 hours of incubation in a growth chamber at 22 ± 2 °C and a photoperiod of 12 hours, we evaluated the mycelial growth of F. moniliforme, and C. gloesporioides. In the last period of incubation, we quantified the production of conidia of each fungus. For the germination test, we added, into the wells of an ELISA test plates, a 40 μL aliquot of each extract at the concentrations of 0, 5, 10 and 20% and another aliquot of a suspension of conidia of each pathogen. After 24 hours at 22 ± 2 °C in the dark, the germination of the fungi was stopped with the addition of 20 μL of lactophenol. Then, we evaluated the germination of conidia. The experiments

Some Plants Showing Antagonism to Five Plant Pathogenic Fungi

“…A study developed by Silva and coworkers (2014), with the 20% extract and paper disc method, ie without autoclaving, indicated a 17.04% inhibition of this fungus. The infusion of lemongrass leaves in PDA medium (autoclaved) led to a median inhibition, being higher at a concentration of 20% when compared to previously reported 5 and 10% (Marcondes et al, 2014). Using the same methodology as in the present study, Rozwalka et al (2008) observed a 26.66% inhibition of Cg growth, isolated from guava.…”

“…The autoclaved extract in question was a good inhibitor of Fox growth (63.75%). No information on antagonistic sage extract was accessed for Fox in the literature; however, against F. solani, median inhibition was observed (Marcondes et al, 2014). The aqueous extract obtained by 50% hot water infusion was not effective in inhibiting Ss (Pansera et al, 2012), as observed in the present study.…”

“…Cg was inhibited by 24.07%; however, using the same methodology as the present study, Rozwalka et al (2008) observed 51.11% inhibition of growth of this Cg guava fungus. Similarly, 10% alcohol extract inhibited 45% growth of this fungus (Bonapaz et al, 2019), and the infusion of rosemary leaves, autoclaved in PDA medium, led to median inhibition of this fungus, being higher in the concentration of 20% when compared to previously reported 5 and 10% (Marcondes et al, 2014). Low inhibition was also observed for autoclaved aqueous extract against Fox (20.37%).…”

“…Regarding Cg, the inhibition was low (3.12%). The sage leaf infusion in PDA medium (autoclaved) led to a median inhibition of this fungus, being higher at 20%, when compared to previously reported 5 and 10% (Marcondes et al, 2014). The dried alcoholic extract, dissolved at 1% in water, inhibited 72% of Cg spore germination (Silva et al, 2009).…”

The use of plant extracts in anthracnose control in species of Helconia (Heliconia psittacorum cv. Golden Torch and Heliconia rostrata)

“…Brito and Nascimento (2015) obtained similar results when they studied plant extract fungitoxic potential on Curvularia eragrostidis and observed that garlic, ginger, neem and citronela, starting at 25% concentration, presented bigger plant toxic effects in the in vitro analysis; reducing mycelial growth and sporulation as well as fungus germination. Marcondes et al (2014) also observed that 20% garlic extract completely inhibited C. gloeosporioidese conidia germination and reduced the number and germination of Fusarium moniliforme conidia.…”