2017
DOI: 10.1590/1982-0224-20170043
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Abstract: This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me 2 SO 4 ) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant d… Show more

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Cited by 6 publications
(14 citation statements)
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“…1). These abnormailites have been associated with a broad variety of conditions including, as examples, rearing systems for Eurasian perch, Perca fluviatilis (Alix et al, 2017), larval rearing temperatures for Atlantic halibut, Hippoglossus hippoglossus L. (Ottesen and Bolla, 1998), embryo cryopreservation practices for streaked prochilod, Prochilodus lineatus (Costa et al, 2017), and, in zebrafish, phenanthroline toxicity (Ellis and Crawford, 2016), influenza A virus infection (Gabor et al, 2014), knockdown or KO of genes related to kidney function or development, respectively (Hanke et al, 2013; Zhang et al, 2018), knockdown of the wwox tumor suppressor gene (Tsuruwaka et al, 2015), deletion of a gene ( pr130 ) encoding a protein essential for myocardium formation and cardiac contractile function (Yang et al, 2016), and mutagenesis of genes involved in thyroid morphogenesis and function (Trubiroha et al, 2018), among others. The edemas may ultimately result from many different proximal causes such as cardiac, kidney, liver or osmoregulatory failure, and researchers are just beginning to develop screens to differentiate between them (Hanke et al, 2013).…”
Section: Discussionmentioning
confidence: 99%
“…1). These abnormailites have been associated with a broad variety of conditions including, as examples, rearing systems for Eurasian perch, Perca fluviatilis (Alix et al, 2017), larval rearing temperatures for Atlantic halibut, Hippoglossus hippoglossus L. (Ottesen and Bolla, 1998), embryo cryopreservation practices for streaked prochilod, Prochilodus lineatus (Costa et al, 2017), and, in zebrafish, phenanthroline toxicity (Ellis and Crawford, 2016), influenza A virus infection (Gabor et al, 2014), knockdown or KO of genes related to kidney function or development, respectively (Hanke et al, 2013; Zhang et al, 2018), knockdown of the wwox tumor suppressor gene (Tsuruwaka et al, 2015), deletion of a gene ( pr130 ) encoding a protein essential for myocardium formation and cardiac contractile function (Yang et al, 2016), and mutagenesis of genes involved in thyroid morphogenesis and function (Trubiroha et al, 2018), among others. The edemas may ultimately result from many different proximal causes such as cardiac, kidney, liver or osmoregulatory failure, and researchers are just beginning to develop screens to differentiate between them (Hanke et al, 2013).…”
Section: Discussionmentioning
confidence: 99%
“…As embryo preservation is the focus of the technique, the achievement of the vitreous solid can only be confirmed when one can guarantee, prior to vitrification, that the exposure of the embryos to the cryoprotectant solution will maintain high survival rates (Costa, Souza, Senhorini, Veríssimo-Silveira, et al, 2018). This previous exposure is crucial given the diffusion of cryoprotectants and resulting embryonic dehydration, potentially controlling the damage promoted by the cold temperatures and the nucleation of ice crystals.…”
Section: Discussionmentioning
confidence: 99%
“…Whereas the first three steps were performed in solutions containing only a main internal cryoprotectant (PROP ‐ concentrations of 2, 3, and 4 M), the fourth step was performed in a high‐osmolarity solution combining internal (PROP + dimethyl sulphoxide—Me 2 SO) and external cryoprotectants (Sucrose ‐ SUC). The final concentration of vitrification solution was PROP 5 M + Me 2 SO 5 M + SUC 0.2 M (Costa, Souza, Senhorini, Veríssimo‐Silveira, et al, ). After the exposition of the embryos to the cryoprotectant solution, eight embryos were aspirated into sterile straws of 0.5 ml (IMV), starting their vigorous movement in liquid nitrogen.…”
Section: Methodsmentioning
confidence: 99%
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