Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae) exposed to cold and heat shocks

Garcia, Luciano de Oliveira; Okamoto, Marcelo Hideo; Riffel, Alessandro; Saccol, Etiane M.H.; Pavanato, María A.; Sampaio, Luís André

doi:10.1590/1982-0224-20140148

Cited by 11 publications

(4 citation statements)

References 29 publications

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“…Our results showed an increasing trend of catalase activity in the gills for 3-and 6-hour exposure period (15°C and 35°C) as compared with the muscle (6 h), indicating that the temperature stress has induced a more stubborn defence in the gills than the muscles. Such a trend was also observed in Paralichthys orbignyanus from a transition of 23°C to 28.8°C (Garcia et al, 2015). In our study, catalase antioxidant activity outweighed the LPO levels of the muscles at both the experimental temperature-time levels indicating the surprising capacity of the fish to overcome stress in a short period.…”

Section: Discussionsupporting

confidence: 83%

Impact of transient temperature disturbance on the oxidative stress indices and glucose levels of juvenile Koi carps (Cyprinus carpio var koi)

Zutshi

Singh

Dasgupta

2020

JoBAZ

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Background: The present study reports the changes in the redox state and glucose activity in gills and muscles of Koi carps, exposed to a short duration of acute thermal stress. The variation in levels of lipid peroxidation (LPO) and glutathione (GSH), catalase (CAT) and glutathione-S-transferase (GST) activity, in addition to glucose levels, were analysed after exposing the fishes to 15°C (low), 25°C (control) and 35°C (high) for a duration of 3 and 6 h. The upper and lower temperatures regimes were decided by the Critical Thermal Limit (CT MAX and CT MIN). Results: The CAT activity was high in both the tissues at 35°C within 3 h, while LPO activity decreased throughout the study when compared with the control group indicating immoderate cellular disturbance and surplus oxidative stress. At 15°C (3 h), tissues reported more glucose compared with the upper thermal slab. GSH increased in both the tissues at 15°C compared with the control for 3 and 6 h. GST activity, however, flourished at 35°C in muscles and gills for the 3-h regime. Conclusion: The current study demonstrated disturbing impacts of temperature on the survivability of the Koi carps in a domesticated environment.

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Section: Discussionsupporting

confidence: 83%

Impact of transient temperature disturbance on the oxidative stress indices and glucose levels of juvenile Koi carps (Cyprinus carpio var koi)

Zutshi

Singh

Dasgupta

2020

JoBAZ

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“…However, HSPs expression is fluctuating at both stresses according to fish species (Werner et al, 2005;Wilde, 1988). Glutathione S-transferases (GST), one of the oxidative stress-related genes, its expression associated with different kinds of stressors (Almroth et al, 2015;Garcia et al, 2015). Also, Metallothioneins (MTs) are a family of stress proteins.…”

Section: Introductionmentioning

confidence: 99%

Thermal Stress of Ambient Temperature Modulate Expression of Stress and Immune-Related Genes and Dna Fragmentation in Nile Tilapia (Oreochromis Niloticus (Linnaeus, 1758))

Ashayeri¹

2017

Appl Ecol Env Res

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Abstract. Owing to its cold-blooded nature, water temperature is a driving force in fish life. The aim of this study is to explore the effect of the thermal stress of ambient temperature during summer and winter in Nile tilapia (Oreochromis niloticus) on heat shock proteins (HSP70, HSP27), oxidative stress genes (MT and GST) and some immune response genes (IL-1, IL-8, and TLR7). As well as to compare the DNA fragmentation at both variant temperatures using RAPD-PCR and micronucleus assay. The fish were collected twice, once at the end of December (winter sample, temperature 14˚C), and the other at August (summer sample, temperature 36˚C). qRT-PCR analysis of mRNA expression for the studied genes were done. HSP70 expression increased equally in gills and liver at both summer and winter, suggesting its advantageous role in fish survival in the labile environment. Its expression exceeds HSP27 level, whose expression increased in summer. GST expression increased in summer in liver than gills and expressed equally at both organs in winter. No differences were observed in MT and immune response related gene expression in both seasons. The very close pattern of RAPD-DNA fragmentation and micronuclei were recorded at both thermal stresses. We conclude that, thermal stress of cold and hot extremes of ambient temperature in Nile tilapia have a nearby role in expression of HSP70, MT, liver GST and immune response related genes. As well as the incidence of micronucleus and DNA fragmentation.

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“…This important action, performed mainly by GST in gills of fish that are exposed to heat thermal stress, may indicate protection from lipid damage and explains why the LPO level presented no variation. Garcia et al (2015) noticed an increase in CAT and GST activities as well as in the levels of thiobarbituric acid reactive substances (TBARS) in the gills of Paralichthys orbignyanus that were exposed to heat thermal stress, indicating that even with the activation of antioxidant defenses, this species failed to prevent oxidative damage. In the present study, the activation of antioxidant defenses was efficient and prevented an increase in TBARS levels.…”

Section: Gill Metabolism Changes Caused By Heat Thermal Stressmentioning

confidence: 99%

Metabolic responses in the gills of Yellowtail Lambari Astyanax lacustris under low‐ and high‐temperature thermal stress

Schleger,

Pereira,

Resende

et al. 2024

J Aqua Anim Hlth

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ObjectiveEctothermic fish are directly affected by temperature changes in the environment. The aim of this study was to evaluate the metabolic responses in the gills of Yellowtail Lambari Astyanax lacustris under thermal stress.MethodsTo this end, we used spectrophotometry to evaluate the biomarkers of carbohydrate and protein metabolism, antioxidant defense, and oxidative damage in fish subjected to low (15°C) and high (31°C) temperatures, with control groups held at 23°C, for 2, 6, 12, 24, 48, and 96 h.ResultThe results showed that cold thermal stress did not change the energy demand, and the antioxidant defense was reduced; therefore, the gills were vulnerable to the action of reactive oxygen species (ROS), presenting increased protein carbonylation at 12 h. With heat thermal stress, a higher energy demand was observed, which was verified by an increase in aerobic metabolism by glycolysis and the citric acid cycle. High‐temperature stress also increased the antioxidant defenses, as verified by the increased activities of glutathione peroxidase, glutathione reductase, and glutathione S‐transferase. However, the antioxidant defense system could not protect tissues from the action of ROS, as protein carbonylation increased at 6 and 24 h, indicating oxidative stress.ConclusionThe results showed that (1) temperature variations caused metabolic adjustments in the gills of Yellowtail Lambari, (2) the adaptive responses were different for winter and summer temperatures, and (3) Yellowtail Lambari recovered homeostasis when subjected to thermal stress, even with the occurrence of oxidative stress.

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Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae) exposed to cold and heat shocks

Cited by 11 publications

References 29 publications

Impact of transient temperature disturbance on the oxidative stress indices and glucose levels of juvenile Koi carps (Cyprinus carpio var koi)

Impact of transient temperature disturbance on the oxidative stress indices and glucose levels of juvenile Koi carps (Cyprinus carpio var koi)

Thermal Stress of Ambient Temperature Modulate Expression of Stress and Immune-Related Genes and Dna Fragmentation in Nile Tilapia (Oreochromis Niloticus (Linnaeus, 1758))

Metabolic responses in the gills of Yellowtail Lambari Astyanax lacustris under low‐ and high‐temperature thermal stress

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