2014
DOI: 10.1590/1982-0224-20130149
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Abstract: Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumieri from this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This re… Show more

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Cited by 2 publications
(3 citation statements)
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“…To gain insight into the venom protein composition, we initially produced a cDNA library from S. plumieri to prospect by random EST the major gland components. While several lectins were identified in spine tissue, none of the readouts provided information on Sp-CTx [ 28 ].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…To gain insight into the venom protein composition, we initially produced a cDNA library from S. plumieri to prospect by random EST the major gland components. While several lectins were identified in spine tissue, none of the readouts provided information on Sp-CTx [ 28 ].…”
Section: Discussionmentioning
confidence: 99%
“…We previously described the production and partial characterization of a cDNA library from venomous tissue of S. plumieri , by using the random sequencing approach, and generated hundreds of partial sequences [ 28 ]. This study aims to identify the coding sequences for S. plumieri toxin, and to verify the presence of determinants attributable to the protein that could be responsible for the pharmacological effects of this toxin.…”
Section: Introductionmentioning
confidence: 99%
“…A preliminary analysis of expressed sequence tags (EST) obtained through a cDNA library from S. plumieri venom revealed that about 30% of the sequences had no similarities with previously described ones, suggesting the presence of unknown genes of potential relevance in the venom gland. In addition, the screening of the library with antibodies against a lectin fraction from S. plumieri  venom has shown that lectin-like genes account for 12% of all transcripts, a finding confirmed by extensive in silico analysis [61]. These constitute the very first steps towards the unraveling of the molecular diversity contained in fish venoms.…”
Section: Molecular Genetics Of S Plumieri Venommentioning
confidence: 98%