Photoinactivation of Candida albicans using methylene blue as photosensitizer

Sousa, João Nilton Lopes de; Queiroga, Bruna Honório de; Kocerginsky, Patrícia de Oliveira; Marinho, Petrusk Homero Campos; Araki, Ângela Toshie

doi:10.1590/1981-863720150003000063028

Cited by 9 publications

(15 citation statements)

References 22 publications

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“…Actually, the PDT with a photosensitizer to eliminate microorganisms is a very interesting option for some authors [17,20,[32][33][34][35][36]. The use of photodynamic therapy (LLLT treatment in the presence of methylene blue photosensitizer) to inhibit the antifungal activity of C. albicans, according to the results obtained in this study, with a value of 100% microbial inhibition (CFU/ ml ± SD = 0.0 ± 0.0), is presented as a promising therapeutic alternative, which is the basis of our research, and is consistent with that described previously by other authors [11,37,38].…”

Section: Discussionsupporting

confidence: 91%

“…Studies on this subject are motivated by the evidence that there is great resistance to conventional antimicrobials between pathogenic microorganisms. Thus, the search for therapeutic alternatives, including the application of laser light, is essential for the treatment of these clinical conditions [11,[16][17][18][19][20][21][22].…”

Section: Discussionmentioning

confidence: 99%

“…Different authors [11,19,37,38] who have worked with similar protocols to those used in our study, and many others who have been published on the application of photodynamic therapy (PDT) to inhibit microbial activity [36,39,40], have shown the effects of PDT on the viability of C. albicans and other fungal microorganisms, using methylene blue as photosensitizer. The results of this study show a maximum value of C. albicans inhibition by PDT (100%), represented by a decrease in CFU/ml, where we can see a similar tendency to other published works and where the percentages of inhibition by PDT range between 74 and 95%.…”

Section: Discussionmentioning

confidence: 99%

“…The PS used was methylene blue (MB). The stock solution of MB was prepared at 300 μg/ml in sterile saline solution at a pH of 7.8 [11] and 100 μl were added to the wells of the Group 3, at room temperature. For Groups 1 and 4, 100 μl of PBS were added.…”

Section: Methodsmentioning

confidence: 99%

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In vitro study of photodynamic therapy with visible laser systems applied to fungal infections

Santiago¹,

Gutknecht

Martín-Carrillo

et al. 2020

Laser Dent Sci

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Purpose Evaluate the "in vitro" effect of the 660-nm laser on the inactivation of planktonic cells of Candida albicans, by the application of 660-nm diode laser light (1) on its own and (2) together with photodynamic therapy using methylene blue. We will also compare the ability of microbial inactivation of the laser over octenidine dihydrochloride disinfectant. Methods This study was conducted in a laminar flow cabinet with 4 groups, divided into two: no laser groups-C. albicans suspension in saline solution (Group 1) and C. albicans suspension, with octenidine dihydrochloride antimicrobial solution (Group 2); laser groups-C. albicans suspension, using a 660-nm diode laser with photodynamic therapy and methylene blue (Group 3), and C. albicans suspension, using a 660-nm diode laser without the presence of methylene blue as photosensitizer (PS) (Group 4). They were seeded in 96 wells where we applied laser light in different light conditions in order to get reliable results. Results The results of this study demonstrate that the application of a 660-nm laser, with and without the presence of a photosensitizer, showed microbial inhibition ability against Candida albicans. Photodynamic therapy using the methylene blue photosensitizer presented an antifungal effect against Candida albicans of 100% efficacy, similar to the results obtained with octenidine dihydrochloride. Conclusions The application of photodynamic therapy has demonstrated to be a good alternative, with an effective antifungal effect against Candida albicans, avoiding the side effects on patients, like those from octenidine dihydrochloride.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

In vitro study of photodynamic therapy with visible laser systems applied to fungal infections

Santiago¹,

Gutknecht

Martín-Carrillo

et al. 2020

Laser Dent Sci

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“…Our results are justified according to Carvalho, et al Which indicate that the greater absorption of light emitted by the laser of lowlevel in the blue of methylene occurs in wavelengths larger than 600nm [26]. Thus, we believe that our results occurred because the exposure of a photo sensitizer to the light emitted by a low-level laser causes chemical reactions capable of causing the destruction of proteins, lipids, nucleic acids and other cellular components, resulting in the destruction of microbial cells [27].…”

Section: Discussionsupporting

confidence: 68%

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

Mm¹

2018

VIJ

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Pressure ulcers are injuries that can cause bacterial proliferation, and when infected are associated with increased morbidity and mortality. Thus, photodynamic therapy becomes an adjunct to the treatment with medications because it has the bactericidal capacity. In this way, this study aims to analyze in vitro the bactericidal effect of methylene blue by associating it with the low-level laser in the wavelength of 830nm to determine which therapeutic doses present better efficacy in the treatment of bacteria pseudomonas aeruginosa. The microbiological material was collected by an aseptic swab and the antimicrobial activity was verified by disc diffusion method using sterile filter paper. Methylene blue was used at concentrations of 0,001% and 0,005% and the low-level laser of 830nm at the intensities of 4J/cm², 8J/cm², 10J/cm² and 14J/cm², being tested in three plates for each dose, as well as the low-level laser and the methylene blue, were tested separately. The results were statistically analyzed by SPSS through the "t-test for dependent samples". It was verified that there was reduction of the bacterial activity with the application of the low-level laser in doses of 10J/cm² (p=0,00) and 14J/cm² (p=0,03) added to them ethylene blue in 0.005% concentration, however, there was a greater reduction of bacterial proliferation in the application of 10J/cm². Thus, it was concluded that the photodynamic therapy applied in the concentration of 0.005% of the methylene blue combined with the low-level laser of 830nm of 10J/cm² and 14J/cm² of intensity, present a reduction in the amount of bacterial proliferation.Once the bacteria multiply in the pressure ulcer, new clinical signs and symptoms are observed, worsening the Research ArticleVolume 2 Issue 1

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Incorporation of acridine orange and methylene blue in Langmuir monolayers mimicking releasing nanostructures

Pivetta

Jochelavicius

Wrobel

et al. 2023

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Photoinactivation of Candida albicans using methylene blue as photosensitizer

Cited by 9 publications

References 22 publications

In vitro study of photodynamic therapy with visible laser systems applied to fungal infections

In vitro study of photodynamic therapy with visible laser systems applied to fungal infections

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

Incorporation of acridine orange and methylene blue in Langmuir monolayers mimicking releasing nanostructures

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