2021
DOI: 10.1590/1981-6723.28320
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Abstract: In this work, it was determined the influence of temperature, pH and storage time on the enzymatic activity and stability of an extracellular fructosyltransferase (FTase E.C.2.4.1.9) from Aspergillus oryzae IPT-301 produced by submerged fermentation. The thermodynamic parameters showed a tendency for increasing enzyme denaturation with the rise in temperature. The maximum transfructosylation activity was obtained at the incubation pH 5.5. During storage at 4 °C, the transfructosylation activity decreased, wher… Show more

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Cited by 2 publications
(18 citation statements)
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“…The initial pH of the culture medium was adjusted to 5.5 before sterilization. The flasks were inoculated with 0.5 mL of a suspension of 10 7 spores mL −1 that had been previously prepared and incubated in a rotary shaker at 30 °C and 200 rpm for 64 h. 4,5,7 After cultivation, the culture broth was vacuum-filtered and the resulting supernatant (containing an activity of 11.74 ± 3.37 U mL −1 of FTase) was used for the immobilization assays.…”
Section: Microorganism and Enzyme Productionmentioning
confidence: 99%
“…The initial pH of the culture medium was adjusted to 5.5 before sterilization. The flasks were inoculated with 0.5 mL of a suspension of 10 7 spores mL −1 that had been previously prepared and incubated in a rotary shaker at 30 °C and 200 rpm for 64 h. 4,5,7 After cultivation, the culture broth was vacuum-filtered and the resulting supernatant (containing an activity of 11.74 ± 3.37 U mL −1 of FTase) was used for the immobilization assays.…”
Section: Microorganism and Enzyme Productionmentioning
confidence: 99%
“…Nevertheless, industrial FOS production is a technique that is economically limited by the use of batch reactors and soluble FTase enzymes of low operational stability 8,9 . The development of the transfructosylation reaction of sucrose in a PBR may increase FOS production volume, facilitate their separation from the biocatalyst, and reduce both their production cost and the product inhibition caused by glucose (reaction by‐product) accumulation 8,10 .…”
Section: Introductionmentioning
confidence: 99%
“…Several studies have reported that immobilized extracellular FTases and whole cells with transfructosylation activity present a higher operational stability than the soluble enzymes 3,9,12‐14 . Whole cells are prominent among these biocatalysts because they provide the reaction medium with enzymes immobilized in their own biomass of origin, and hence, do not require purification and immobilization steps 15,16 .…”
Section: Introductionmentioning
confidence: 99%
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