Microencapsulation of Lactiplantibacillus plantarum with inulin and evaluation of survival in simulated gastrointestinal conditions and roselle juice

Abstract

This study aimed to evaluate the survivability of Lactiplantibacillus plantarum 299v encapsulated in chitosan-coated calcium alginate beads with inulin as prebiotic in simulated gastrointestinal conditions and roselle juice. The concentration of calcium chloride and inulin for L. plantarum 299v microencapsulation was optimised and the survivability of free and microencapsulated L. plantarum was assessed under simulated gastrointestinal conditions. Storage stability of the optimised encapsulated L. plantarum 299v-inulin was determined throughout four (4) weeks of storage in roselle juice at 4 °C and 25 °C. The optimized formula for L. plantarum 299v was 2.0% (w/v) of calcium chloride and 3.0% (w/v) of inulin. Optimized calcium alginate-chitosan L. plantarum 299v microbeads with inulin did not affect (p > 0.05) the bead diameter, with a mean diameter of 685.27 μm, and microencapsulation efficiency of 95%. Encapsulated L. plantarum 299v with inulin showed higher survivability (>107 CFU/mL) than free cells and encapsulated L. plantarum 299v without inulin under simulated gastrointestinal conditions and after four (4) weeks of storage in roselle juice at 4 °C. The results indicate that co-extrusion encapsulation and addition of inulin had improved the viability of L. plantarum 299v in roselle juice by protecting probiotic against unfavourable gastrointestinal conditions and prolonged storage.

Effective encapsulation of reuterin-producing Limosilactobacillus reuteri in alginate beads prepared with different mucilages/gums

“…The use of gums and mucilage in the composition of the beads conferred even greater protection to L. reuteri cells compared to Control (p<0.05), which showed reduction of 26.38%, while TG, MM, CG, PM and KG showed an average reduction of 12.55, 14.67, 13.67, 12.96 and 13.6%, respectively. The sharped decline in the viability of bacterial cells subjected to gastric juices were generally attributed to the acidic environment and presence of digestive enzymes [ [60] , [61] ]. Similar to our results, Nami et al.…”

Physicochemical properties of heterogeneously acetylated glucomannan of A. oncophyllus and its performance for iron encapsulation

“…In drug-delivery use, the properties of matching matrix allow to support the drug-targeted delivery, which maintains and releases the active substances in specified organs (Wang et al, 2019). Chean et al (2021) used chitosan-alginate matrix to protect the Lactiplantibacillus plantarum against simulated gastrointestinal conditions.…”