Study of the Molecular Weight of Pullulan Produced by Aureobasidium pullulans from Industrial Waste

Abstract

The objective of this research was the sustainable development of pullulan and its structural and rheological characterization. In addition, the cultivation of the A. pullulans Y2092 strain by submerged fermentation on a laboratory scale was evaluated, valuing the use of industrial waste (residual brewery's yeast), RBY, and a low-cost renewable raw material VHP (very high polarization sugar). A 2 2 factorial statistical design of experiments was used to analyze the molecular weight of the pullulans produced. The maximum value of 1955 kDa was determined for the pullulan recovered after 96 h of fermentation in the mineral medium added with 70 g/L of VHP and 50 g/L of RBY. The FTIR analysis for the pullulan obtained was similar to the commercial pullulan. The rheological behavior of pullulan solutions is shown, evidencing the relationship between this property and M w . The results from all analyses indicate that the pullulan obtained is suitable for the future production of biopolymeric films.

A Novel Fabrication of Heterogeneous Saponified Poly(Vinyl Alcohol)/Pullulan Blend Film for Improved Wound Healing Application

“…Figure 4b,c also show a similar trend regarding the saponified films, but the peak intensity varied depending on the PVAc/PULL ratio, which might be due to the change in material property. However, when FT-IR spectra of the unsaponified films were considered, Figure 4c shows a very precise broad peak in the range of 3000-3600 cm −1 , as PULL also contains the -OH group [28]; it was also revealed that PVA was obtained from PVAc in the presence of PULL.…”

Biopolymers as Seed-Coating Agent to Enhance Microbially Induced Tolerance of Barley to Phytopathogens

“…The dynamic viscosity of the polymer's solution with different concentrations-2, 4, 6, 8, 10, and 12% w/v-was performed using a rotational Ametek Brookfield DVPlus viscometer with a ULA spindle, at different shear rates, ranging from 0.1 to 500 s −1 at 25 • C [23]. The viscosimetric analyses of the samples were performed at 25 • C.…”