Different methods of processing thermographic images to evaluate the carpal temperature of healthy calves

Abstract

The aim of this study was to compare three methods of processing thermographic images for the evaluation of carpal temperature in healthy calves. Additionally, we sought to evaluate the thermal symmetry between the contralateral carpi of these calves. Thermographic images were obtained from both carpi of eight healthy calves aged between 20 and 45 days with a mean weight of 38.65 ± 2.27 kg. Using GRAYESS® IRT Analyzer 7 software, the total temperature (Ttot), region-of-interest temperature (Troi), and maximum mean temperature (Tmax) were determined. There was no difference between the temperatures obtained by the Ttot and Troi methods. Tmax showed higher temperatures (p < 0.01) than the other methods. The three image processing methods showed high and significant positive correlations for the temperature of the right and left carpi of healthy calves. Tmax presented a higher correlation coefficient (r = 0.99) than the two other methods, which suggests a greater sensitivity for identifying thermal variations among the contralateral carpi. The 99% confidence interval for the difference between the temperatures of the right and left carpi (∆r-l) was between 0.03 and 0.26°C; thus, a temperature variation within that range does not refer to pathologic processes.