Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL

Poester, Vanice Rodrigues; Mendes, Josiara Furtado; Groll, Andréa von; Klafke, Gabriel Baracy; Brandolt, Tchana Martinez; Xavier, Melissa Orzechowski

doi:10.1590/1809-6891v19e-52571

Cited by 5 publications

(7 citation statements)

References 21 publications

(36 reference statements)

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“…In the present study, this method was inefficient to detect S. brasiliensis DNA in the environmental material. Poester et al. (2018) also observed negative DNA by species-specific PCR and nested PCR for the five Sporothrix species ( S .…”

Section: Discussionmentioning

confidence: 89%

“…from nature may be related to the limitations of the available detection methods or the paucity of environmental studies on the sporotrichosis agents. Poester et al. (2018) evaluated the presence of Sporothrix spp.…”

Section: Discussionmentioning

confidence: 99%

“…in soil samples from places in Southern Brazil where zoonotic sporotrichosis also occurs. Despite the 101 samples collected from the residence of cats with sporotrichosis, none of the cultures were positive for Sporothrix spp., which could be related to the moderate growth of Sporothrix spp, making their detection in soil samples difficult due to the abundant growth of numerous other saprobic microorganisms ( Poester et al., 2018 ). Rodrigues et al.…”

Section: Discussionmentioning

confidence: 99%

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Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Rabello

Almeida-Silva

Scramignon-Costa

et al. 2022

Front. Cell. Infect. Microbiol.

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Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of Sporothrix spp. in the environment from an area of the Rio de Janeiro state, Brazil, with recurrent cases of human and animal sporotrichosis. Abandoned demolition timber wood samples were collected in the garden of a house where the cases of human and feline sporotrichosis have occurred in the last 10 years. The environmental survey revealed a Sporothrix spp. colony from the serial dilution cultures of one abandoned demolition wood sample. In addition, a fungal strain isolated from a cat with skin lesions that lived in the house was also included in the study. The species-specific PCR, and calmodulin partial sequencing identified the environmental and cat isolates as S. brasiliensis. Furthermore, the phylogenetic analysis performed with the partial sequences of internal transcribed spacer region and constitutive genes (calmodulin, β-tubulin, and chitin synthase) showed high similarity between environmental and cat isolates from the same geographic region. Moreover, the antifungal susceptibility test revealed that the minimal inhibitory concentration of itraconazole from the environment isolate was lower than the cat isolate, while amphotericin B and terbinafine were similar. Our results show that S. brasiliensis is able to maintain itself in the environmental material for years. With this, we corroborate that the eco-epidemiology of sporotrichosis is not well understood, and despite the major occurrence of S. brasiliensis in Brazil, it is rarely isolated from the environment.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Rabello

Almeida-Silva

Scramignon-Costa

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Thus, investigative studies on the presence of pathogenic fungi in feline claws are important, since many of them as already described, cause important clinical changes in cats, they can also cause zoonoses, such as Sporothrix sp. (POESTER et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Imprint and spreading techniques for the isolation and identification of subungueal fungi in claws of healthy cats

Oliveira

Carvalho

Castro

et al. 2021

AVB

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The aim of this study was to compare imprint and spreading techniques for the isolation and identification of colonies of pathogenic and non-pathogenic fungus in the claws of semidomiciliated cats. For that propose, 150 cats were evaluated, subdivided into three groups of 50 animals. In the first and second groups, the cats were submitted to the imprint technique in Petri dishes containing Selective Mycobiotic Agar: In the first group, the cats were subjected underwent antisepsis with 70% ethanol of the claws of the thoracic limbs and in the second group the animals were subjected underwent antisepsis with 70% ethanol of the claws of only one of the thoracic limbs. The third group was submitted to the spreading technique, whose material was collected by rubbing a sterile swab moistened with brain-heart infusion broth, in the claws of the forelimbs, where an aliquot of the material was transferred to Petri dishes containing Selective Mycobiotic Agar. The material was stored at 25°C for 30 days. The readings were performed on days 5, 7, 15, and 30 post incubation. Using the imprinttechnique performed under the conditions of this experiment, we were not able to isolate and identify the colonies because since day 5, they were overlapped. From the spreading technique, Mucor sp. (54,34%), Rhodotorula sp. (28,26%), Fusarium sp. (21,73%), Aspergillus sp. (21,73%), Trichoderma sp. (19,56%), Penicillium sp. (19,56%), Cladosporium sp. (10,86%), Rhizopus sp. (8,68%), Acremonium sp. (6,5%), Exophialia sp. (6,5%), Paecilomyces sp. (4,34%), Trichosporon sp. (4,34%), and Geotrichum sp. (2,17%) were isolated. It was concluded that the spreading technique proved to be useful in isolating fungal colonies from feline claws, and the animals do not present symptoms, which signals the importance of them as possible sources of exposure for tutors. The cats were negative for Sporothrix sp. by the imprint and spreading techniques.

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“…The increase in disease prevalence is due to many factors including the virulence of the pathogen -S. brasiliensis is the most virulent species in the S. schenckii complex (ARRILLAGA-MONCRIEFF et al, 2009;FERNANDES et al, 2013;DELLA TORRE et al, 2017) -difficulty for treating this mycotic disease, therapeutic time frame, zoonotic potential, and the large numbers of non-domiciled and semidomiciled (stray) cats that are affected by this fungal infection. All these factors make sporothricosis a public health problem in Brazil, which is especially alarming in the States of Rio de Janeiro, Minas Gerais and Rio Grande do Sul (POESTER et al, 2018;GREMIÃO et al, 2020;MACÊDO-SALES et al, 2020;NAKASU et al, 2020;RODRIGUES et al, 2020).…”

Section: Sporotrichosismentioning

confidence: 99%

Thymomodulin in association with antifungal drugs in the therapy of cats with disseminated cutaneous sporotrichosis: a prospective study

et al. 2021

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Feline sporotrichosis is a relevant mycose in veterinary medicine due to its severity and zoonotic potential and the fact that it can be difficult to treat. The immune status of the animal exerts influence on the prognosis of the disease and determines its clinical outcome. This study evaluated the efficacy of the immunomodulatory thymomodulin as an adjunct to antifungal therapy in cats with disseminated sporotrichosis; thymomodulin was used in association with itraconazole (ITL) and potassium iodide (KI) to treat this fungal disease in the feline patient. Thirty-one cats (n=31) diagnosed with disseminated cutaneous sporotrichosis were divided into two groups as follows: Group 1 (G1) (n=16), which included those animals that were treated with thymomodulin in association with ITL and KI, and Group 2 (G2) (n=15) which had pacientsthat received ITL and KI only. The response to different treatment modalities was assessed, considering the survival rate, time frame for the lesions to respond to therapy, and clinical improvement or deterioration according to a body condition score system. Animals from G1 had a survival rate of nearly 100% (93.6%) that was approximately twice higher than the survival rate of those animals from G2 (53%). Moreover, patients from G1 had a significantly better prognosis, improved body condition, and shorter time for remission of the extra cutaneous clinical signs (p<0.02). Our findings showed that the association of thymomodulin with ITL and KI improves the prognosis of cats with disseminated cutaneous sporotrichosis.

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Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL

Cited by 5 publications

References 21 publications

Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Imprint and spreading techniques for the isolation and identification of subungueal fungi in claws of healthy cats

Thymomodulin in association with antifungal drugs in the therapy of cats with disseminated cutaneous sporotrichosis: a prospective study

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