Lesões cutâneas tipo tumorais associadas à infeção por avipoxvirus em uma marreca-cabocla (Dendrocygna autumnalis)

Pereira, Washington Luiz Assunção; Gabriel, Áurea Martins; Monger, Suellen da Gama Barbosa; Moraes, Leopoldo Augusto; Queiroz, Darlene Kássia Saraiva; Souza, Alex Júnior Souza de

doi:10.1590/1809-6891v15i217202

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…Although it is not a highly lethal disease and shows an atypical occurrence, avianpox disease is being frequently reported in avian wildlife in Brazil (Catroxo et al 2009, Vargas et al 2011, Pereira et al 2014. In broilers, skin lesions caused by avian poxvirus were detected in carcasses of slaughtered birds from unvaccinated flocks (Fallavena et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

et al. 2018

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This study describes an outbreak of avian poxvirus disease in previously pox-vaccinated turkeys in Brazil. The turkeys had suggestive gross lesions of cutaneous avian poxvirus in the skin of the head and cervical area without changes in the flock mortality rates. In the slaughterhouse, 30 carcasses were removed from the slaughter line to collect tissue from cutaneous lesions for histological analyses and characterization of the virus. The virus was identified by conventional polymerase chain reaction (PCR) and subsequent gene sequencing. Acanthosis, hyperkeratosis, and hydropic degeneration were seen on skin histopathology. Eosinophilic intracytoplasmic inclusion bodies (Bollinger) on keratinocytes were observed in 46.6% of the samples. Avian poxvirus DNA was detected on PCR in 83.3% of the total samples. PCR associated with histopathology had 93.3% of positivity for avian poxvirus. In the phylogenetic study, samples show 100% matching suggesting that the outbreak occurred by a single viral strain and was different from those strains affecting other wild birds such as canaries and sparrows. A single mutation (Adenine for Guanine) was detected in our study’s strain and in the strains of turkey, chickens, and vaccine strains published in GenBank. Also, when the sequence strain of the present study and sequences from GenBank of canarypox and sparrowpox strains were aligned, a Thymine was found replacing the Adenine or Guanine. The in ovo vaccination method as single-use in turkeys of this study apparently did not provide adequate protection against avianpox disease, but additional vaccination administered by wing-web when turkeys were 45-60 days old in the new flocks controlled the disease. In the subsequent year, new cases of this disease were not found. It was not possible to confirm the source of the virus strain, but infection with a field strain derived from chickens is one possibility, considering the poultry farm population in the area and biosecurity aspects. For wide characterization of avipoxvirus and differentiation among strains, the complete sequence of the viral genome is required.

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Section: Introductionmentioning

confidence: 99%

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

et al. 2018

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“…This report provides the first molecular characterization of Avipoxvirus detected in tumor-like lesions in avian species. While previous reports have described simultaneous occurrences of SCC or tumor-like lesions and poxvirus detection in lesions in black-bellied whistling duck (Dendrocygna autumnalis) (Pereira et al 2014) and in broilers in Brazil (Fallavena et al. 2002), and pink-backed pelican in Italy (Pesaro et al 2009), there is a lack of molecular characterization of these viruses.…”

Section: Discussionmentioning

confidence: 99%

“…Tumor-like lesions were also reported in the skin of young Chilean flamingos (Arai et al 1991), in the cornea, bursa of Fabricius, in the lungs of Galapagos doves (Mete et al 2001) and canaries (Ritchie & Carter 1995), and head bones of rosy-faced lovebirds (Tsai et al 1997). In Anseriformes, poxvirus infection was reported to be associated (Pereira et al 2014) and not associated (Morton & Dieterich 1979) with cutaneous tumor-like lesions.…”

Section: Introductionmentioning

confidence: 99%

Avipoxvirus detected in tumor-like lesions in a white-faced whistling duck (Dendrocygna viduata)

et al. 2020

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Avipoxvirus is the etiological agent of the avian pox, a well-known disease of captive and wild birds, and it has been associated with tumor-like lesions in some avian species. A white-faced whistling duck (Dendrocygna viduata) raised in captivity was referred to a Veterinary Teaching Hospital in Northeast due to cutaneous nodules present in both wings. A few days after the clinical examination, the animal died naturally. Once submitted to necropsy, histopathological evaluation of the lesions revealed clusters of proliferating epithelial cells expanding toward the dermis. Some of these cells had round, well-defined, intracytoplasmic eosinophilic material suggestive of poxvirus inclusion (Bollinger bodies). PCR performed on the DNA extracted from tissue samples amplified a fragment of the 4b core protein gene (fpv167), which was purified and sequenced. This fragment of Avipoxvirus DNA present in these tumor-like lesions showed high genetic homology (100.0%) with other poxviruses detected in different avian species in several countries, but none of them were related to tumor-like lesions or squamous cell carcinoma. This is the first report of Avipoxvirus detected in tumor-like lesions of a white-faced whistling duck with phylogenetic analysis of the virus.

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“…Silva et al (2009) reports the presence of Fowlpox in backyard chickens by clinical signs and confirming the diagnosis by histopathology. Vargas et al (2011) reported an infection by Avian poxvirus in barn owl, and Pereira et al (2014) reported a cutaneous tumor-like lesions associated with infection by APV in a Dendrocygna autumnalis. Both studies confirmed the diagnosis by clinical signs as well as by histopathology.…”

Section: Discussionmentioning

confidence: 99%

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

et al. 2016

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RESUMO.-[Primeira análise filogenética de Avipoxvirus (APV) no Brasil.] Este trabalho representa a primeira aná-lise filogenética de Poxvirus aviário detectado em perus no Brasil. Os distúrbios clínicos relacionados com bouba aviá-ria aqui descritos ocorreram em um sistema de alojamento de perus. As aves apresentaram lesões características de varíola observadas no pescoço, pálpebras e bico das aves. Quatro perus com sinais característicos foram escolhidos aleatoriamente, sacrificados e submetidos à autópsia. This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank ® . The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species. . Estudos adicionais, como isolamento viral, PCR e sequenciamento, incluindo um grande número de perus da produção brasileira devem ser conduzidos devido ao grave risco que a infecção por poxvírus pode causar ao cenário de produção avícola brasileira, tendo em vista que a produção brasileira de perus atrai atenção devido a sua importância mundial. Nossos resultados apontam para a necessidade de identificar a prevalência da APV na produção de peru no Brasil, para realizar estudos de avaliação de risco e continuada monitoração de infecções por APV nas espécies de aves comerciais e silvestres.

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Lesões cutâneas tipo tumorais associadas à infeção por avipoxvirus em uma marreca-cabocla (Dendrocygna autumnalis)

Cited by 4 publications

References 17 publications

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys

Avipoxvirus detected in tumor-like lesions in a white-faced whistling duck (Dendrocygna viduata)

First phylogenetic analysis of Avipoxvirus (APV) in Brazil

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