Evaluation of the micropropagation potential of curauá pineapple hybrids for fiber production

Abstract

Plant fiber is a renewable and biodegradable material that can be used effectively to reinforce various composites. Pineapple hybrids selected for their fiber quality are in the phase of agronomic validation in Brazil by the Embrapa Cassava and Fruits research unit. The selection of a hybrid for large-scale fiber production depends on obtaining a large number of seedlings. This study evaluated the morphogenetic response and propagation potential of eight hybrids of Ananas comosus var. erectifolius, for the purpose of producing high-quality seedlings on a large scale. Stem and crown buds were reduced and placed in MS nutritive medium supplemented with BAP at 0.5 mg L-1, NAA at 0.01 mg L-1 and Phytagel® at 2.5 g L-1. After 45 days, the number of oxidized, contaminated and surviving buds was determined. Swollen buds and plantlets were transferred to a multiplication medium containing MS sucrose, salts and vitamins. The propagation potential was evaluated based on the geometric growth rate among sub-cultures. The FIB-NEG hybrid presented the best results for the establishment phase (40.28%). The best propagative potential was obtained from crown buds with the highest values for FIB-EST (3.93), FIB-MIN (3.91) and FIB-BOY (3.91) hybrids.

Vegetative propagation strategies in commercial pineapple cultivars

“…The geometric growth rates found in this study show an atypical behavior of what has been shown in other studies, such as Silva et al (2016) and Souza et al, (2018), which observed a decrease in propagation potential in pineapple after the third subculture.…”

In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra

“…The selection of the explant and the culture medium are basic conditions for establishing an in vitro propagation protocol or in vitro conservation method (JIANG et al, 2012). The cultivation medium, MS (MURASHIGE &SKOOG, 1962), has proven to be efficient and responsive in most herbaceous species such as banana, pineapple, and strawberry (GALAN et al, 2018;SOUZA et al, 2018;MOZAFARI et al, 2018). However, woody species have other needs, especially for basic salts.…”