Prevalência da infecção pelo Vírus da Leucose dos Bovinos em rebanhos leiteiros criados no estado do Amazonas, Brasil

Carneiro, Paulo A.M.; Araújo, Wanderley Pereira de; Birgel, Eduardo Harry; Souza, Kleberson Worslley de

doi:10.1590/1809-4392200331125

Cited by 12 publications

(17 citation statements)

References 15 publications

(4 reference statements)

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“…Concerning the outbreaks, the percentage found (95% -19/20) was similar to that obtained in Maranhão (98.9%) [36] and in Amazon region (100%) [6] but discretely superior to other Brazilian surveys, ranging from 60 to 85% [13,23]. This finding reflects the degree of negligence regarding the prophylactic measures of EBL since it is a silent propagation disease, as well as the implications for the absence of official control and eradication programs in Brazil [28,35].…”

Section: Discussionsupporting

confidence: 82%

“…The frequency of EBL in the Rio Branco microregion (14.42%) is below the estimated national average (33.9%), as well as the regional level [31]. According to the latest epidemiological surveys of the northern states, seropositivity was 8.9% in Amazonas [6], 49.8% in Pará [23], 23% in Rondônia [22], and 27.8% in Tocantins [13]. The reduced influx of animals due to the geographical situation of Acre, since it is an area of greater physical isolation due to forest and river barriers, may influence the observed frequency.…”

Section: Discussionmentioning

confidence: 99%

“…According to the classification proposed by Shettigara et al [38], the Rio Branco microregion can currently be considered as an area of medium frequency for BLV infection, a situation that ranges from 11 to 30% in the occurrence rate. When compared to herds with low infection frequency (<10%), the elimination of seroreagent animals and their respective outbreaks become more expensive in areas of medium or high frequency [6].…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosis and control of EBL are neglected, allowing their dissemination, especially in developing countries [32,40]. In the Brazilian Amazon, prevalences ranging from 8.9% [6] to 49.8% [23]. However, the description of the epidemiological characteristics relevant to the transmission of EBL and its impacts on the herds it is not clarified.…”

Section: Introductionmentioning

confidence: 99%

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Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon

Silva

Melchior

Reckziegel

et al. 2019

Acta Scientiae. Vet.

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Background: Enzootic bovine leukosis (EBL) is a viral and lymphoproliferative disease that attributes negative economic impacts to dairy farming. The immunosuppressive condition of these bovines favors the development of bacterial diseases such as tuberculosis, brucellosis, and bovine leptospirosis, which implications to the public health given that they are zoonosis. Diagnosis and control of EBL are neglected in cattle rearing, allowing their dissemination, especially in developing countries. In this sense, the objective of this study was to analyze the risk factors of EBL in dairy cattle from the Brazilian Amazon. Materials, Methods & Results:In order to identify the BLV-infected animals (527 serological samples), we used the agarose gel immunodiffusion serological test (IDGA) to diagnose the EBL and characterize the risk factors through the application of epidemiological questionnaires. We used the logistic regression test to evaluate the existence of a significant association between the variables. The EBL frequency in the study area was 14.42%. We detected seroreagent bovines in all municipalities evaluated with 95% of the properties showing outbreaks of the disease. The reuse of needles was the risk factor with the most significant statistical association (P < 0.01; OR = 2.16). Discussion: The origin of EBL in the Brazilian Amazon is probably related to the expansion of dairy cattle in the 1980s, which, at first, demanded the importation of cattle for the fast formation of the herds, without complying with the sanitary criteria of animal commercialization. The frequency of EBL in the Rio Branco microregion is below the estimated national average, as well as the regional level. The reduced influx of animals due to the geographical situation of Acre, since it is an area of greater physical isolation due to forest and river barriers, may influence the observed frequency. Different environmental conditions, herd size, cattle management, genetics and particularities of the diagnostic techniques should also be considered. The Rio Branco microregion can currently be considered as an area of medium frequency for BLV infection, a situation that ranges from 11 to 30% in the occurrence rate. When compared to herds with low infection frequency (<10%), the elimination of seroreagent animals and their respective outbreaks become more expensive in areas of medium or high frequency. As for the number of outbreaks detected, the finding reflects the degree of negligence regarding the prophylactic measures of EBL since it is a silent propagation disease, as well as the implications for the absence of official control and eradication programs in Brazil. According to the final logistic regression model, the reuse of needles for vaccinations, deworming, and for applying other drugs in different animals was the risk factor of greater association in the region, explaining the widespread occurrence of EBL in the studied farms. In Canadian herds, this practice also was associated with an increase in clinical cases. It is known that ...

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon

Silva

Melchior

Reckziegel

et al. 2019

Acta Scientiae. Vet.

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“…No presente estudo, os resultados obtidos relacionados ao fator sexo dos animais demonstraram haver uma diferença significativa entre a prevalência de machos e fêmeas, contrariando os resultados de trabalhos realizados no Paraná, Amazonas e Piauí (26,28,29) .…”

Section: Resultsunclassified

Inquérito Soroepidemiológico Da Leucose Enzoótica Bovina Em Rebanhos Bovinos Do Estado De Sergipe

Batista

Costa

et al. 2018

Ciênc. anim. bras.

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ResumoSergipe, localizado na região Nordeste do Brasil, é o único estado dessa região com ausência de estudos sobre a soroprevalência da leucose enzoótica bovina, havendo a necessidade da realização de pesquisas para verificação da situação atual da enfermidade. O objetivo do presente estudo foi realizar um inquérito soroepidemiológico da leucose enzoótica bovina em rebanhos bovinos do estado de Sergipe, dividido em três mesorregiões: Leste Sergipano, Agreste Sergipano e Sertão Sergipano. Em cada mesorregião foram selecionados os cinco municípios com o maior efetivo bovino, totalizando 780 amostras coletadas, dentre machos e fêmeas, de faixas etárias diversas, provenientes de 52 propriedades dos municípios visitados. Todas as amostras de soro foram submetidas ao teste de imunodifusão em gel de ágar (IDGA), obtendo-se resultados positivos para anticorpos antivírus da leucose bovina (anti-VLB) em 11,92% (93/780) dos animais. Dentre as propriedades, 59,61% (31/52) apresentaram animais reagentes, sendo que, dos 15 municípios visitados, apenas dois não apresentaram animais sororreagentes. De acordo com os resultados obtidos, ficou confirmada a presença de anticorpos contra o agente etiológico nos rebanhos bovinos sergipanos, reforçando a necessidade de instituir medidas de controle e prevenção eficazes contra a leucose bovina, com o objetivo de controlar a doença no estado e conhecer melhor a sua epidemiologia. Palavras-chave: bovinos; IDGA; soroprevalência. AbstractThe state of Sergipe, located in the Northeast of Brazil, is the only state in the region with absence of studies on the seroprevalence of Enzootic Bovine Leukosis. Therefore, there is a need of research to verify the current status of the disease. The aim of this study was to conduct a seroepidemiological inquiry of enzootic bovine leukosis in cattle herds in the state of Sergipe, which was divided into three mesoregions: East Sergipe, Agreste Cienc. anim. bras., Goiânia, v.19, 1-12, e-23196, 2018Inquérito soroepidemiológico da leucose enzoótica bovina em rebanhos bovinos do Estado de Sergipe Sergipe (dry and wild area of Sergipe) and Sertão Sergipe (arid, badlands of Sergipe). In each mesoregion, we selected the five counties with the largest cattle population, totaling 780 samples collected from males and females at all ages, from 52 farms of the visited counties. All serum samples were submitted to the test of agar gel immunodiffusion (AGID) and positive results were obtained for anti-virus antibodies of bovine leukosis (anti-VLB) in 11.92% (93/780) of the animals. Among the farms, 59.61% (31/52) presented seropositive animals, and from the 15 counties visited, only two of them did not show seropositive animals. According to the results, the presence of the etiologic agent in cattle in Sergipe was confirmed, reinforcing the need to institute effective measures of control and prevention against Enzootic bovine, in order to control the disease in the state and better understand its epidemiology.

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Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis

et al. 2020

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Enzootic bovine leukosis (EBL) is an infectious disease caused by bovine leukemia virus (BLV) that affects cattle worldwide. Agar gel immunodiffusion (AGID) was the reference test for EBL diagnosis for many years, but enzyme-linked immunosorbent assay (ELISA) showed higher sensitivity, was faster to perform, and resulted in an objective reading. However, the importation of ELISA kits is lengthy and expensive, and currently, no AGID kits are available in Brazil. The aim of this work was to standardize an indirect ELISA (iELISA) for EBL diagnosis using BLV antigens produced in Tadarida brasiliensis lung (Tb1Lu) cells, which are Bovine viral diarrhea virus (BVDV) free, unlike fetal lamb kidney (FLK) cells, currently used for this purpose. Following standardization, iELISA results were compared with those obtained by AGID and the commercial Chekit Leucose-Serum ELISA. Compared to AGID, iELISA had 94,44% sensitivity, 75.68% specificity, 79.10% positive predictive value (PPV) and 93.30% negative predictive value (NPV), with 84% concordance and a Kappa index of 0.699. Compared to the Chekit Leucose-Serum ELISA, iELISA showed 92.60% sensitivity, 87.09% specificity, 90.27% PPV and 90,00% NPV, with 90.27% concordance and a Kappa index of 0.801. Taking into account the high agreement with the traditional tests and the absence of non-specific reactions with BVDV, the developed assay could be used as diagnostic method to control EBL in Brazil.

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Prevalência da infecção pelo Vírus da Leucose dos Bovinos em rebanhos leiteiros criados no estado do Amazonas, Brasil

Cited by 12 publications

References 15 publications

Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon

Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon

Inquérito Soroepidemiológico Da Leucose Enzoótica Bovina Em Rebanhos Bovinos Do Estado De Sergipe

Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis

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