Padrões ontogenéticos das Esterases, Leucina Aminopeptidase e X-Glicerofosfato Desidrogenase em Anopheles (Nyssorhynchus) Albitarsis lynch-arribálzaga, 1878 (Diptera: Culicidae)

Abstract

RESUMO -Modificações na expressão gênica foram observadas nos sistemas esterase, leucina aminopeptidase e x-glicerofosfato desidrogenase, durante o desenvolvimento ontogenético de Anopheles albitarsis. A esterase revelou quatro regiões de atividade, sendo a esterase 1 detectada apenas em larvas de 4º estádio velhas e em pupas, as esterases 2 e 4 foram presentes durante todo o desenvolvimento, e a esterase 3 revelou-se praticamente apenas em larvas e raríssimas vezes em pupas. Também foram observadas quatro regiões de atividade na leucina am presente somente em pupas e adultos e a LAP4 foi detectada nos três diferentes estágios. Uma única região foi observada para a x-glicerofosfato desidrogenase e a intensidade de sua atividade cresce à medida que se aproxima o estágio adulto. Palavras-chave:Anopheles albitarsis, isoenzimas, ontogenia, malária, Amazônia. Ontogenetic Patterns of Esterases, Leucine Aminopeptidase and x-Glycerophosphate DehydrogenABSTRACT -Modifications in genetic expression were observed in the esterase, leucine albitarsis. The esterase revealed four regions of activity. Esterase 1 was detected in 4th stage larvae and in

Ontogenetic patterns and genetic variation in Anopheles (Anopheles) intermedius Chagas, 1908 and Anopheles (Anopheles) mattogrossensis Lutz & Neiva, 1911 (Diptera: Culicidae) in the Brazilian Amazon

“…The electrophoretic patterns of α−glycerophosphate dehydrogenase isozymes during the ontogeny of A. intermedius and A. mattogrossensis -showing weak staining intensity in 4thinstar larvae with increasing activity in pupae and adults -are similar to those obtained by Narang et al (1979), Santos et al (1996b) and Maia & Santos (1999). These authors detected only one region, for this enzyme, whose activity increased until the adult stage of A. aquasalis, A. darlingi and A. albitarsis.…”

“…The results concerning ontogeny of leucine aminopeptidase in A. intermedius and A. mattogrossensis are similar to those obtained by Maia & Santos (1999) in A. albitarsis. This author detected four activity regions: LAP1 and LAP2 was observed in the 4th-instar larvae; LAP3, was found only in pupae and adults; LAP4 was detected throughout development.…”

“…Samples 1 to 5 = 4 th-instar larvae; 6 to 9 = pupae; 10 to 13 = adults. Maia (1997) and Maia & Santos (1999) detected four activity regions for esterases during ontogeny of Anopheles albitarsis; EST1 was seen in the 4th-instar old larvae and pupae, EST2 and EST4 during all developmental stages, and EST3 was only detected in 4th-instar larvae. Similar results were obtained by Santos (1992) and Santos et al (1996a,b) who detected five activity regions during ontogeny in Anopheles darlingi: EST1 and EST2 showed the highest staining intensity in the larval stages, whereas EST3 and EST4 were most deeply stained in pupae and adults; EST5 was detected during all developmental stages.…”

“…The activity pattern of each enzyme is result of the role that it has according to its developmental stage. Vedbrat & Whitt (1975), Santos et al (1996a, b) and Maia & Santos (1999). Vedbrat & Whitt (1975) and Maia Santos (1999).…”

“…Several studies have pointed out that some enzymes are modified during the development either in terms of band intensity or in terms of the appearence of new forms and the desappearence of others (Wright & Shaw, 1969;O'Brien & MacIntire, 1972;Vedbrat & Whitt, 1975;Santos, 1979;Santos et al, 1985;Santos et al, 1996a,b;Scarpassa, 1988;Scarpassa et al, 1992;Maia & Santos, 1999). These studies, when taken as whole, enable a deeper understanding of how the genes in mosquitoes act and are expressed.…”