Evaluation of a semi-quantitative method for rapid screening of plant phenolic content

Abstract

A rapid method for screening phenolic content in leaves is described and evaluated. The method is based on a treated paper strip that is squeezed around a leaf with pilars. Weaknesses of the method are some subjectivity, lack of precision, and apparent insensitivity to phenolics present as glycosides. Strengths include its speed and independence of laboratory facilities. Immediate testing of leaves following detachment appears to be essential. The crude extraction in the field compares favorably with a more conventional extraction technique. The method is recommended for use in preliminary screening of plant material when a large number of species must te examined within a short time and when laboratory facilities are not available at the field site.