Prevalência De Anticorpos Contra Agentes Virais Em Equídeos No Sul Do Estado De São Paulo

Abstract

RESUMO Foram analisados soros de 163 equídeos (143 equinos e 20 muares) provenientes de 16 municípios do sul do Estado de São Paulo, parte no Vale do Ribeira e parte no litoral. Utilizouse uma amostra probabilística estratificada em dois estágios, sendo a unidade amostral de primeiro estágio constituída pela unidade de produção agropecuária (UPA) e a de segundo estágio pelo animal dentro da UPA sorteada. Os soros foram coletados no período de outubro de 2004 a junho de 2005. Foram estimadas as prevalências de anticorpos contra os vírus das encefalites equinas do tipo leste (EEE), oeste (WEE) e venezuelana (VEE), herpesvírus equino (HVE), arterite viral dos equinos (AVE), influenza equina 1 e 2 (IE-1 e 2) e estomatite vesicular Indiana 2 – Cocal (COCV) e Indiana 3 – Alagoas (VSAV). Foram utilizadas as técnicas de inibição da hemoaglutinação para a detecção de anticorpos contra os vírus IE-1 e 2 e de soroneutralização para os demais vírus estudados. Dos animais testados, 26% apresentaram anticorpos contra o HVE, 21% para COCV, 5% para VSAV, 16% para EEE, 2,26% para VEE, 11% para influenza Equi/2 e 2,7% para influenza Equi/1. Não se encontraram animais reagentes para WEE e AVE. A soroprevalência obtida sugere a circulação do HVE, dos vírus da IE Equi-1(H7N7) e Equi-2 (H3N8), dos vírus EEE e VEE e dos vírus da estomatite vesicular Indiana 2 – Cocal (COCV) e Indiana 3 – Alagoas (VSAV) no rebanho de equídeos do sul do Estado de São Paulo.

Seroprevalence and risk factors associated with seropositivity for equine encephalomyelitis virus in horses in Rio Grande do Norte, Brazil

“…Results for seroprevalence in other regions of the country, such as those reported in the state of São Paulo, 2.26% (CUNHA et al, 2009), andRondônia, 11.3% (AGUIAR et al, 2008), were lower than in this study. However, reported frequencies of seropositive equines in Minas Gerais, in the south of the state of Paraná, and in the Pantanal region of Mato Grosso were 32.1%, 27.37%, and 21.0%, respectively (HEINEMANN et al, 2006;AGUIAR et al, 2008;LARA et al, 2014).…”

“…Serological detection and isolation of the virus has been reported in several regions and demonstrates how widespread the virus is in Brazil, with studies from Pará (HEINEMANN et al, 2006;CAMPOS et al, 2013), Rondônia (AGUIAR et al, 2008), Ceará (SILVA et al, 2011), Paraíba (ARAÚJO et al, 2012), Pernambuco (PIMENTEL et al, 2009, Mato Grosso (MELO et al, 2012), Distrito Federal (SOUSA et al, 2015, Minas Gerais (LARA et al, 2014), São Paulo (KOTAIT et al, 1992CUNHA et al, 2009) and Paraná (FERNÁNDEZ et al, 2000). The presence of these agents in domestic herds should be frequently monitored because of the possible economic and social losses incurred from infection (LARA et al, 2014).…”

“…Serum samples were sent to the Rabies and Viral Encephalitis Laboratory of the São Paulo Biological Institute for serological diagnosis by investigating neutralizing antibodies against EEEV, WEEV, and VEEV using the seroneutralization technique described by CUNHA et al (2009). Sera were tested at dilutions of 1:5 for detection of antibodies against EEEV, WEEV, VEEV using a 100 DICT50/25 μL suspension of the Eastern (Tatuí sample), Western (TR 25717 sample), and Venezuelan complex (SP AN 15600 sample) viruses stored at the Biological Institute of São Paulo.…”

Serological evidence of Eastern equine encephalitis circulation in equids in Pará state, Brazil

“…Previous serological surveys reported the prevalence of antibodies against EEEV in the Brazilian Amazon and other Brazilian states, including studies by Aguiar et al (2008 ) in the state of Rondônia (21% prevalence); Cunha et al (2009 ) in the state of São Paulo (16% prevalence); Pauvolid-Corrêa et al (2010 ) in the South Pantanal (47.7% prevalence); Melo et al (2012 ) in the Amazon, Pantanal, and Cerrado biomes of the state of Mato Grosso (35.5% prevalence); Lara et al (2014 ) in the state of Minas Gerais (30.2% prevalence); and Pauvolid-Corrêa et al (2015) in the state of Mato Grosso do Sul (18% prevalence).…”

Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil

“…An early serosurvey in 1940-41 in the State of Minas Gerais (southeast (SE) region) performed during an epizootic with high case-fatality rates showed a seropositivity of 44.4% among alive equines [24]. In a region historically affected by EEEV epizootics in the State of São Paulo (SE region), seropositivity in equines was 16% in 2004-2005 [25]. In 1993, in the State of Mato Grosso (midwest (MW) region) 6.4% among hundreds of surveyed healthy equines were seropositive for EEEV in an area with reports of equine encephalitis cases [26], while in 2007 47.7% of non-vaccinated equines were seropositive in the same region [27].…”