How has dental pulp stem cells isolation been conducted? A scoping review

Ferrúa, Camila Perelló; Centeno, Eduarda Gervini Zampieri; Rosa, Laísa Camerini da; Amaral, Cainá Corrêa do; Severo, Rafaely Ferreira; Sarkis‐Onofre, Rafael; Nascimento, Gustavo G.; Cordenonzi, Gabriele; Krolow, Rachel; Demarco, Flávio Fernando; Nedel, Fernanda

doi:10.1590/1807-3107bor-2017.vol31.0087

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…Over 20 different enzymes and their combinations were considered during the DPSC dissociation methods. Collagenase I associated with dispase and collagenase alone were the most frequently used methods [16]. A recent research showed it will enrich more cells with CD146+, which were considered as the mainly DPSCs marker, as well as osteogenic and chondrogenic ability [14].…”

Section: Isolation Culture and Cryopreservation Of Dpscsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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Adult stem cells are excellent cell resource for cell therapy and regenerative medicine. Dental pulp stem cells (DPSCs) have been discovered and well known in various application. Here, we reviewed the history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage. Moreover, we discussed the future potential application of the combination of tissue engineering and of DPSC differentiation. This review will help the new learner to quickly get into the DPSC filed.

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Section: Isolation Culture and Cryopreservation Of Dpscsmentioning

confidence: 99%

Recycle the dental fairy’s package: overview of dental pulp stem cells

Yang

Xiao

et al. 2018

Stem Cell Res Ther

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“…7,11 Therefore, in order to fully exploit these desirable DPSCs for regenerative purposes, it is imperative that strategies are developed which permit the optimisation of population selection through the selectively screening and isolation of superior quality DPSCs from dental pulp tissues for in vitro expansion, assessment and prudent cell banking; thereby aiding the translational development of more effective DPSC-based therapies for clinical evaluation and application. [12][13][14] However, conventional ex vivo cellular expansion and stem cell/senescence characterisation techniques used to identify superior DPSCs are often expensive, labour-intensive and time-consuming; whilst cellular expansion can also detrimentally alter stem cell characteristics leading to reduced regenerative properties. 11,15 Such issues may be confounded by the requirement for invasive fixation, staining or cell permeabilisation techniques, which damage cellular components during MSC characteristation.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Dental Pulp Stem Cell Regenerative Heterogeneity by Single-Cell Raman Spectroscopy

Alraies

Canetta²,

Waddington

et al. 2019

Tissue Engineering Part C: Methods

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Dental pulp stem cells (DPSCs) are increasingly being recognized as a viable cell source for regenerative medicine. However, significant heterogeneity in their ex vivo expansion capabilities are well-established, which influences their regenerative and therapeutic potentials. As highly proliferative/multi-potent DPSCs are minority sub-populations within dental pulp, the development of non-invasive strategies capable of successfully discriminating between DPSC sub-populations with contrasting proliferative and differentiation capabilities in situ, would be immensely beneficial for the selective screening/isolation of superior quality DPSCs for in vitro assessment and therapy development. Consequently, this study assessed the effectiveness of Single Cell Raman Spectroscopy (SCRM), in distinguishing between DPSC sub-populations with contrasting proliferative and differentiation capabilities isolated from dental pulp tissues. Individual DPSC sub-populations were isolated from human third molars and identified as high or low proliferative and multi-potent or uni-potent, following in vitro expansion and senescence confirmation. High proliferative/multi-potent DPSCs, such as A3 (18PDs and 60PDs) and low proliferative/uni-potent DPSCs, including A1 and B1 (8PDs and 7PDs respectively), were analysed using an iHR550 Raman Spectrometer, equipped with a CCD camera and Eclipse Ti-U Inverted Microscope. Single cell spectra were acquired for 20 cells in each sub-population (10 spectra per nuclear and 10 spectra per cytoplasmic/membrane regions in each cell analysed), over 500-2100 cm-1. Spectra and peak assignments were obtained, followed by PCA and multivariate statistical analysis. Although DPSC spectra contained typical Raman peaks for nucleic acids, proteins and lipids, the spectral intensities of high proliferative/multi-potent DPSCs, A3 (18PDs), were higher than A3 (60PDs), A1 (8PDs) and B1 (7PDs), reflecting significantly elevated DNA (729 cm-1) and protein (1111, 1167, 1245 and 1680 cm-1) contents overall. PCA and multivariate analysis revealed significant variations in scatter plots and Raman signatures, with distinct fingerprints for high proliferative/multi-potent DPSCs, A3 at 18PDs and 60PDs; versus the similar overlapping profiles for low proliferative/uni-potent DPSCs, A1 3 (8PDs) and B1 (7PDs). This study confirms that SCRM successfully discriminates between DPSC sub-populations with contrasting proliferative and differentiation capabilities, advocating its further assessment as a viable technique for the selective non-invasive screening, identification and isolation of high proliferative/multi-potent DPSCs from dental pulp tissues for regenerative medicine applications. Impact Statement This study is the first to investigate and confirm the effectiveness of Single Cell Raman Spectroscopy (SCRM), in its ability to discriminate between DPSCs with contrasting proliferative and differentiation capabilities. The findings show that SCRM can rapidly and noninvasively distinguish and identify DPSC sub-populations in vit...

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“…Reports on enzymatic methods for pulp extraction hold practical implications including non -standardized enzyme concentrations which potentially might induce cell death [23]. Even though, mixture of collagenase and other proteinases aids digestion of the pulp, and if associated with mechanical instruments improves cell dissociation; type of association technique, filtering, medium supplementation and size of the tissue fragments all in total create bias reported in method sections [24]. Although, both methods, outgrowth and enzymatic digestion allow harvesting multipotent lineages of pulp cell, authors favour the latter over the former due to better characteristics of yielded cells [25].…”

Section: Culture and Isolationmentioning

confidence: 99%

Human Dental Pulp Stem Cells: recent findings and current research

Janowicz

Mozdziak

Bryja

et al. 2019

Medical Journal of Cell Biology

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Prevalence of neurodegenerative diseases, most of which are life threatening and incurable, is an increasing clinical problem. To date, studies have demonstrated a superior proliferation rate of dental pulp stem cells (DPSCs) compared to other mesenchymal stem cells in vitro. DPSCs has recently been recognized as a novel treatment strategy for neurodegenerative disease, due to their advanced potential for neurogenic differentiation. The oral cavity has been described as a promising source of dental pulp stem cells. DPSCs are widely used in regenerative dentistry holding alternative capacity for osteogenic differentiation and therefore new promises for tissue and whole tooth regeneration. Dental stem cell banking offers a plentiful source of stem cells representing great potential for cell reprogramming and thus cell therapy. Recently, the association of pulp stem cells with three – dimensional scaffold templates allows for building up naturally derived implants. This review introduces to unique properties of DPSCs and biological factors influencing mineralization, proliferation and differentiation of pulp stem cells. Latest research studies are compared in terms of effectiveness and limitations of techniques for the isolation of pulp stem cells, including the enzymatic digestion and the explant culture methods. Moreover, a short overview of most recent findings and clinical application of DPSCs is proffered including progress of current research and limitations still to be addressed in the nearest future. Finally, the article presents new advances in the area of regenerative dentistry and regenerative medicine, including three dimensional printing and three dimensional analysis, emerged to deepen studies under procedures to replace the non patient specific artificial implants.Running title: DPSCs - review

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How has dental pulp stem cells isolation been conducted? A scoping review

Cited by 20 publications

References 39 publications

Recycle the dental fairy’s package: overview of dental pulp stem cells

Recycle the dental fairy’s package: overview of dental pulp stem cells

Discrimination of Dental Pulp Stem Cell Regenerative Heterogeneity by Single-Cell Raman Spectroscopy

Human Dental Pulp Stem Cells: recent findings and current research

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