Expression of CD90 and P75NTR stem cell markers in ameloblastomas: a possible role in their biological behavior

Silva, Fernanda Paula Yamamoto; Dias, A.T.; Coelho, Carolinne Almeida; Guerra, Eliete Neves Silva; Marques, Ana Elizia Mascarenhas; Decúrcio, Daniel de Almeida; Mantesso, Andréa; Cury, Sérgio Elias Vieira; Silva, Brunno Santos de Freitas

doi:10.1590/1807-3107bor-2016.vol30.0109

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…It takes part in cellular migration and fibrosis, both of which are important events for tissue regeneration and oncogenesis (40). Its expression in tumor stroma, especially in endothelial and mesenchymal stem cells, including tumor-associated fibroblasts, appears to be an influential agent in disease progression (41). Stromal expression of CD90 in our POT cases was found in blood vessels and in some mesenchymal cells, particularly in those in close proximity to epithelial odontogenic cells, which led us to suggest that CD90 expression in POT could play an important role in cellular adhesion and migration, serving as an important regulator of cell-matrix interactions for determining the biological behavior of this tumor.…”

Section: Discussionmentioning

confidence: 99%

Primordial odontogenic tumor: An immunohistochemical profile

Bologna‐Molina

Mikami²,

Pereira-Prado³

et al. 2017

Med Oral

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BackgroundPrimordial Odontogenic Tumor (POT) is a recently described odontogenic tumor characterized by a variably cellular loose fibrous tissue with areas similar to the dental papilla, covered by cuboidal to columnar epithelium that resembles the internal epithelium of the enamel organ, surrounded at least partly by a delicate fibrous capsule. The purpose of this study was to investigate the possible histogenesis and biological behavior of this rare tumor by means of a wide immunohistochemical analysis of its epithelial and mesenchymal components.Material and MethodsThe immunoexpression of twenty-three different antibodies were evaluated in four cases of POT.ResultsThe epithelial cells that cover the periphery of the tumor showed immunopositivity for Cytokeratins 14 and 19, while Amelogenin, Glut-1, MOC-31, Caveolin-1. Galectin-3, PITX2, p53, Bax, Bcl-2, Survivin and PTEN were variably expressed in focal areas. The mesenchymal component of the tumor was positive for Vimentin, Syndecan-1, PITX2, Endoglin (CD105), CD 34, Cyclin D1, Bax, Bcl-2, Survivin and p53. PTEN and CD 90 showed a moderate positivity. BRAF V600E and Calretinin were negative in all samples. Cell proliferation markers (Ki-67, MCM-7) were expressed in <5% of the tumor cells.ConclusionsAccording to these immunohistochemical findings, we may conclude that POT is a benign odontogenic tumor in which there is both epithelial and mesenchymal activity during its histogenesis, as there is expression of certain components in particular zones in both tissues that suggests this tumor develops during the immature (primordial) stage of tooth development, leading to its inclusion within the group of benign mixed epithelial and mesenchymal odontogenic tumours in the current World Health Organization classification of these lesions. Key words:Immunohistochemistry, jaw tumors, odontogenic, primordial.

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Section: Discussionmentioning

confidence: 99%

Primordial odontogenic tumor: An immunohistochemical profile

Bologna‐Molina

Mikami²,

Pereira-Prado³

et al. 2017

Med Oral

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“…The second most popular subtype is the plexiform type, which is composed of anastomosing strands of ameloblastomatous epithelium . Stem cell markers of CD90 were reported to express differentially depending upon pathological types .…”

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confidence: 99%

Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model

et al. 2017

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Ameloblastoma is a benign tumor of the odontogenic epithelium with several histological subtypes. All subtypes of ameloblastoma contain abundant stroma; the tumor cells invade collectively into the surrounding tissues without losing intratumor cell attachments. However, the molecular mechanisms mediating ameloblastoma invasion remain unclear. Here, we evaluated the functional significance of the interactions between ameloblastoma tumor cells and stromal fibroblasts on collective cellular invasion using a three‐dimensional cultivation method, double‐layered collagen gel hemisphere ( DL ‐ CGH ) culture. The AM ‐1 plexiform and AM ‐3 follicular human ameloblastoma cell lines and HFF ‐2 human fibroblasts were labeled with GFP and DsRed, respectively. Collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM ‐1 cells formed sharp, plexiform‐like invasive processes, whereas AM ‐3 cells formed a series of blunt processes often observed during collective migration. In comparison, under the cocultures with HFF ‐2 fibroblasts, AM ‐3 cells formed tuft‐like invasive processes and collectively invaded into outer layer more than that observed with AM ‐1 cells. Moreover, HFF ‐2 fibroblasts localized to the tips of the invasive tumor processes. These findings suggest that tumor‐associated cells assist tumor cell invasion. Microscopic analysis of sectioned three‐dimensional cultures revealed that AM ‐3/ HFF ‐2 hemispheres were histologically similar to follicular ameloblastoma tumor samples. Therefore, our findings suggest that ameloblastoma subtypes exhibit distinct invasion patterns and that fibroblasts promote collective tumor invasion in follicular ameloblastoma.

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“…In AB, different studies confirmed the expressions of stem cell markers in different locations. While the tumor stem cells are suggested to be located in the peripheral layers (20), another study demonstrate considerable expression of the P75NTR stem cell marker mostly in regions resembling the stellate reticulum (21). Further studies observed the stem cell indicators in both the stellate reticulum like cells and the peripheral basal cells of ABs (22,23).…”

Section: Discussionmentioning

confidence: 87%

Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

2019

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The etiology and pathogenesis of odontogenic lesions are poorly understood. Keratin 15 (K15) is a type I cytoskeletal protein that provides structural support to the cells and has been considered to be a stem cell marker. The aim of the present study was to evaluate the expression of K15 in the epithelial lining of dentigerous cysts (DCs), odontogenic keratocysts (OKCs) and ameloblastomas (ABs). The study included 41 samples of DCs (n=13), OKCs (n=12), and AB tissues (n=16). K15 protein expression was evaluated via immunohistochemistry and data were statistically analyzed using a Kruskal-Wallis test. K15 was expressed in the majority of the studied lesions with various distributions in the different study samples. The Kruskal-Wallis test revealed non-significant differences in the expression of K15 among the three odontogenic lesions (P=0.380). The present study confirmed the high expression of K15 in the different epithelial layers of DC, OKC and AB. This type of expression excludes the reliability of regarding K15 as a stem cell marker in DC, OKC and AB. However, K15 may reflect the abnormal differentiation of pathological epithelial cells in these lesions.

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Expression of CD90 and P75NTR stem cell markers in ameloblastomas: a possible role in their biological behavior

Cited by 9 publications

References 29 publications

Primordial odontogenic tumor: An immunohistochemical profile

Primordial odontogenic tumor: An immunohistochemical profile

Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model

Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

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