Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

Abstract

Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

Biomateriais usados na revascularização pulpar: revisão de literatura

“…Biodentine é um cimento reparador de silicato tricálcio (Ca3SiO5) baseado na composição do cimento Portland e desenvolvido na tentativa do fabricante de melhorar suas propriedades fisico-químicas e biológicas. O produto consiste de um pó (silicato tricálcio, carbonato de cálcio e óxido de zircônia como radiopacificador) e um líquido (contendo cloreto de cálcio e um polímero solúvel em água) que são misturados para formar o cimento (Nai et al, 2016;Quintana et al, 2019).…”

“…Por outro lado não foram demonstrados efeitos genotóxicos no teste do cometa. Não há na literatura estudos in vivo avaliando a exposição a diferentes doses de Ca3SiO5 (Nai et al, 2016).…”