2016
DOI: 10.1590/1807-3107bor-2016.vol30.0080
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Abstract: Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cells, which could improve tissue engineering. SHEDs (eighth passage) were seeded into 96-well culture plates (10 3 cells/well) and were grown in culture medium supple… Show more

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Cited by 33 publications
(24 citation statements)
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“…PBMT was applied with an energy density of 5 J/cm 2 , which elicited positive effects on cell proliferation (Moura‐Netto, Ferreira, Maranduba, Mello‐Moura, & Marques, ), migration and differentiation when associated with growth factors (Diniz et al, ). Moreover, in vivo, PBMT under these parameters has shown to promote the regeneration of dental pulp tissue in rats (Moreira et al, ).…”
Section: Discussionmentioning
confidence: 99%
“…PBMT was applied with an energy density of 5 J/cm 2 , which elicited positive effects on cell proliferation (Moura‐Netto, Ferreira, Maranduba, Mello‐Moura, & Marques, ), migration and differentiation when associated with growth factors (Diniz et al, ). Moreover, in vivo, PBMT under these parameters has shown to promote the regeneration of dental pulp tissue in rats (Moreira et al, ).…”
Section: Discussionmentioning
confidence: 99%
“…Laser irradiation was carried out for 10 s in the dark, using an indium-gallium-aluminum phosphide (InGaAlP) red laser (660 nm: MMOptics, São Carlos, São Paulo, Brazil) [14][15][16]. The irradiation was punctual and in contact with the bottom of the wells, as described previously [10][11][12][13][14][15][16]. For groups I to IV, cells were kept under nutritional deficit (1% FBS) during irradiation and for the remaining time of the experimental period (24, 48, or 72 h).…”
Section: Nutritional Deficit and Low-level Laser Therapy Conditionsmentioning
confidence: 99%
“…If properly stimulated, SHED are able to differentiate into odontoblasts and endothelial cells [7][8][9][10][11][12], representing useful candidates for dental tissue regeneration. Although mesenchymal cells from various sources have been subjected to laser irradiation to stimulate their regenerative potential [8][9][10][11][12], the effect of PBMT on SHED has been poorly studied [6,10,12,13]. In particular, the assessment of SHED viability after laser irradiation under different culture conditions has been limited [6].…”
Section: Introductionmentioning
confidence: 99%
“…Finally, the third published study was done by our group confirming that 5 J cm −2 is an energy density that could be useful during tissue engineering when SHEDs are applied in nutritional-deficient areas [42]. In the studies done until 2015, the cells were cultured in 2D, on the bottom of culture flasks.…”
Section: Pbmt and Dentoalveolar-derived Mesenchymal Stem Cellsmentioning
confidence: 99%