Fungal Community Structure as an Indicator of Soil Agricultural Management Effects in the Cerrado

Valadares-Pereira, Alana de Almeida; Oliveira, Elainy Cristina Alves Martins; Navarrete, Acácio Aparecido; Junior, Waldesse Piragé de Oliveira; Tsai, Siu Mui; Pelúzio, Joênes Mucci; Morais, Paula Benevides de

doi:10.1590/18069657rbcs20160489

Cited by 5 publications

(4 citation statements)

References 34 publications

(38 reference statements)

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“…The prevalence of ascomycetes has been correlated with human activity and the high tolerance to environmental stresses by representatives of the phylum ( Souza et al, 2016 ). The change in agricultural management of Cerrado soil caused changes in the structure of the fungal community of the soil, mainly due to changes in soil properties, such as nutritional levels, CEC, and base saturation ( Valadares-Pereira et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Short-Term Effect in Soil Microbial Community of Two Strategies of Recovering Degraded Area in Brazilian Savanna: A Pilot Case Study

et al. 2021

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The Brazilian Cerrado is a highland tropical savanna considered a biodiversity hotspot with many endemic species of plants and animals. Over the years, most of the native areas of this biome became arable areas, and with inadequate management, some are nowadays at varying levels of degradation stage. Crop-livestock integrated systems (CLIS) are one option for the recovery of areas in degradation, improving the physicochemical and biological characteristics of the soil while increasing income and mitigating risks due to product diversification. Little is known about the effect of CLIS on the soil microbial community. Therefore, we perform this pilot case study to support further research on recovering degraded areas. The bacterial and fungal soil communities in the area with CLIS were compared to an area under moderate recovery (low-input recovering - LI) and native savanna (NS) area. Bacterial and fungal communities were investigated by 16S and ITS rRNA gene sequencing (deep rRNA sequencing). Ktedonobacteraceae and AD3 families were found predominantly in LI, confirming the relationship of the members of the Chloroflexi phylum in challenging environmental conditions, which can be evidenced in LI. The CLIS soil presented 63 exclusive bacterial families that were not found in LI or NS and presented a higher bacterial richness, which can be related to good land management. The NS area shared 21 and 6 families with CLIS and LI, respectively, suggesting that the intervention method used in the analyzed period brings microbial diversity closer to the conditions of the native area, demonstrating a trend of approximation between NS and CLIS even in the short term. The most abundant fungal phylum in NS treatment was Basidiomycota and Mucoromycota, whereas Ascomycota predominated in CLIS and LI. The fungal community needs more time to recover and to approximate from the native area than the bacterial community. However, according to the analysis of bacteria, the CLIS area behaved differently from the LI area, showing that this treatment induces a faster response to the increase in species richness, tending to more accelerated recovery. Results obtained herein encourage CLIS as a sustainable alternative for recovery and production in degraded areas.

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Section: Discussionmentioning

confidence: 99%

Short-Term Effect in Soil Microbial Community of Two Strategies of Recovering Degraded Area in Brazilian Savanna: A Pilot Case Study

et al. 2021

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“…A soil sampling was carried out by tracing an imaginary cross of 50 meters on each side to mark five points (intersection and ends of the cross) to achieve soil physical-chemical characterization. At each point, a composite soil sample was collected consisting of five sub-samples of surface soil (0 to 10 cm depth), also arranged on the ends (04) and intersection (01) of a 2-meter-long cross (Valadares-Pereira et al, 2017). At these same five points, ten undisturbed surface soil samples (two at each point) were extracted in metallic cylinders of 5 cm in diameter and 5 cm in height for physical analysis.…”

Section: Study Site and Soil Samplingmentioning

confidence: 99%

Pores distribution influences the soil microorganism's response to changes in temperature and moisture

Visconti-Moreno

Valenzuela-Balcázar

2023

EJSS

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Microorganisms are an essential fraction of soil organic matter, which presence and activity depend directly on soil physical conditions. This study aimed to address the effect of soil temperature and moisture under contrasting macroporosity conditions on soil biological properties. Soil physical-chemical characterization implicated the collection of composite samples and undisturbed surface soil samples (0 to 10 cm). Also, samples of undisturbed surface soil were extracted in 40 polyvinyl chloride cylinders of 18 cm diameter and 20 cm height for the arrangement of soil mesocosm as the experimental units of a completely randomized experiment with a 2x2x3 factorial arrangement. The experiment duration was 21 days, and the soil biological properties measured were microbial biomass (MB) and soil respiration (SR). Macroporosity showed a significant effect on MB, which indicates that aeration pore influences the number of microorganisms in the soil; for the SR, the macroporosity had a not significant effect. The temperature at the ranges established in the experiment did not significantly affect MB, whereas a highly significant effect of temperature over SR was observed. A highly significant effect of soil moisture was observed on MB and SR. Macroporosity, moisture, and temperature are determining factors in the presence of soil microorganisms, both directly and through the interaction between them. Herein the microorganisms have a wide range of thermal adaptation, and the effect of soil temperature can boost soil microorganisms. In turn, it was observed that the microorganisms present are significantly sensitive to the moisture deficit in soil.

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“…The choice of the DNA extraction method is a crucial step in molecular studies, which can affect the detection of microbial communities' structure and composition ( _ Inceoǧlu et al, 2010;Terrat et al, 2012;Hallmaier-Wacker et al, 2018). The DNeasy PowerLyzer PowerSoil Kit (Qiagen) is one of the most used commercial kits for direct DNA extraction from tropical soils (e.g., Navarrete et al, 2015b;de Araujo et al, 2017;Goss-Souza et al, 2017;Valadares-Pereira et al, 2017;Lammel et al, 2018;Portilho et al, 2018), including Amazonian samples (e.g., Mirza et al, 2014;Paula et al, 2014;Hamaoui et al, 2016;Meyer et al, 2017). However, considering the heterogeneous physicochemical properties of these environments and that each soil type should have its own optimized DNA protocol respecting its unique composition and biomass abundance (Narayan et al, 2016), no work has been done so far to improve this extraction method for samples from several Amazonian land-uses.…”

Section: Dna Concentration and Puritymentioning

confidence: 99%

Robust DNA protocols for tropical soils

Venturini

Nakamura

Gontijo

et al. 2020

Heliyon

Self Cite

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Studies in the Amazon are being intensified to evaluate the alterations in the microbial communities of soils and sediments in the face of increasing deforestation and land-use changes in the region. However, since these environments present highly heterogeneous physicochemical properties, including contaminants that hinder nucleic acids isolation and downstream techniques, the development of best molecular practices is crucial. This work aimed to optimize standard protocols for DNA extraction and gene quantification by quantitative real-time PCR (qPCR) based on natural and anthropogenic soils and sediments (primary forest, pasture, Amazonian Dark Earth, and v arzea, a seasonally flooded area) of the Eastern Amazon. Our modified extraction protocol increased the fluorometric DNA concentration by 48%, reaching twice the original amount for most of the pasture and v arzea samples, and the 260/280 purity ratio by 15% to values between 1.8 to 2.0, considered ideal for DNA. The addition of bovine serum albumin in the qPCR reaction improved the quantification of the 16S rRNA genes of Archaea and Bacteria and its precision among technical replicates, as well as allowed their detection in previously non-amplifiable samples. It is concluded that the changes made in the protocols improved the parameters of the DNA samples and their amplification, thus increasing the reliability of microbial communities' analysis and its ecological interpretations.

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Fungal Community Structure as an Indicator of Soil Agricultural Management Effects in the Cerrado

Cited by 5 publications

References 34 publications

Short-Term Effect in Soil Microbial Community of Two Strategies of Recovering Degraded Area in Brazilian Savanna: A Pilot Case Study

Short-Term Effect in Soil Microbial Community of Two Strategies of Recovering Degraded Area in Brazilian Savanna: A Pilot Case Study

Pores distribution influences the soil microorganism's response to changes in temperature and moisture

Robust DNA protocols for tropical soils

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