Knockout of p16INK4a promotes aggregative growth of dermal papilla cells

Cheng, Yi; Gao, Yang; Zhao, Lu; Gao, Shunqiang; Zhang, Guoqiang; Zhang, Yan

doi:10.1590/1806-9282.63.10.883

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…In the experimental group, after H19 was overexpressed from 3rd passage DP cells onward, the cell shape, proliferation and multi-layer aggregation were maintained until passage-6. Notably, the ability of DP cells to induce HF formation is dependent on their aggregative growth (Cheng et al, 2017). Moreover, HF-inducing ability is observed with 4th passage DP cells, overexpressing H19, but not with control DP cells in passage 4, following injection into NU/NU mice.…”

Section: Discussionmentioning

confidence: 94%

LncRNA H19 Overexpression Activates Wnt Signaling to Maintain the Hair Follicle Regeneration Potential of Dermal Papilla Cells

et al. 2020

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Androgenetic alopecia (AGA) is a common hair loss disorder resulting in seriously abnormal social interaction and psychological disorders. Transplantation with autologous dermal papilla cells represents a prospective therapy. However, the ability of dermal papilla cells to induce hair follicle development is lost upon cell culturing. Long non-coding RNAs (lncRNAs) are an important class of genes involved in various biological functions, are aberrantly expressed in disease and may play roles in the regulation of Wnt signaling, a critical pathway in maintaining the hair follicle-inducing capability of dermal papilla cells. Examination of dermal papilla cells by lncRNA microarray revealed that H19 was highly expressed in early passage dermal papilla cells compared with late-passage dermal papilla cells. In this study, we constructed H19-overexpressing dermal papilla cells to examine the role of H19 on hair follicle inductivity. Dermal papilla cells infected with lentivirus encoding H19 maintained their cell shape, and continued to display both multiple-layer aggregation and hair follicleinducing ability upon prolonged culture. H19 exerted these effects through inducing miR-29a to activate Wnt signaling by directly downregulating the expression of Wnt suppressors, including DKK1, Kremen2, and sFRP2, thereby forming a novel regulatory feedback loop between H19 and miR-29a to maintain hair follicle-inducing potential. These results suggest that lncRNA H19 maintains the hair follicle-inducing ability of dermal papilla cells through activation of the Wnt pathway and could be a target for treatment of androgenetic alopecia.

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Section: Discussionmentioning

confidence: 94%

LncRNA H19 Overexpression Activates Wnt Signaling to Maintain the Hair Follicle Regeneration Potential of Dermal Papilla Cells

et al. 2020

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“…Moreover, knocking out p16 INK4a promoted faster growth of hair follicle dermal papillary cells and an increase in the number of cells in the G1 phase (Cheng et al, 2017).…”

Section: Role Of Cellul Ar S Ene Scen Ce In S Kin Patholog Ic Al Cond...mentioning

confidence: 96%

“…This phenomenon was associated with increased SA‐β‐Gal and p16 INK4a /pRb expression (Bahta et al, 2008). Moreover, knocking out p16 INK4a promoted faster growth of hair follicle dermal papillary cells and an increase in the number of cells in the G1 phase (Cheng et al, 2017).…”

Section: Role Of Cellular Senescence In Skin Pathological Conditionsmentioning

confidence: 99%

Cellular senescence in skin‐related research: Targeted signaling pathways and naturally occurring therapeutic agents

et al. 2023

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Despite the growing interest by researchers into cellular senescence, a hallmark of cellular aging, its role in human skin remains equivocal. The skin is the largest and most accessible human organ, reacting to the external and internal environment. Hence, it is an organ of choice to investigate cellular senescence and to target root‐cause aging processes using senolytic and senomorphic agents, including naturally occurring plant‐based derivatives. This review presents different aspects of skin cellular senescence, from physiology to pathology and signaling pathways. Cellular senescence can have both beneficial and detrimental effects on the skin, indicating that both prosenescent and antisenescent therapies may be desirable, based on the context. Knowledge of molecular mechanisms involved in skin cellular senescence may provide meaningful insights for developing effective therapeutics for senescence‐related skin disorders, such as wound healing and cosmetic skin aging changes.

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Knockout of p16INK4a promotes aggregative growth of dermal papilla cells

Cited by 2 publications

References 21 publications

LncRNA H19 Overexpression Activates Wnt Signaling to Maintain the Hair Follicle Regeneration Potential of Dermal Papilla Cells

LncRNA H19 Overexpression Activates Wnt Signaling to Maintain the Hair Follicle Regeneration Potential of Dermal Papilla Cells

Cellular senescence in skin‐related research: Targeted signaling pathways and naturally occurring therapeutic agents

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