Genotype 1 of human parvovirus B19 in clinical cases

Abstract

Introduction: Virus surveillance strategies and genetic characterization of human parvovirus B19 (B19V) are important tools for regional and global control of viral outbreak. In São Paulo, Brazil, we performed a study of B19V by monitoring the spread of this virus, which is an infectious agent and could be mistakenly reported as a rash and other types of infection. Method: Serum samples were subjected to enzyme immunoassay, real time polymerase chain reaction, and sequencing. Results: From the 462 patients with suspected cases of exanthematic infections, the results of the 164 serum samples were positive for B19V immunoglobulin M. Among these cases, there were 38 patients with erythema infections and B19--associated with other infections such as encephalitis, hydrops fetalis, chronic anemia, hematological malignancies. These samples were sequenced and identified as genotype 1. Conclusion: This study showed patients with infections caused by B19V and sequencing genotype 1. Continuous monitoring is necessary to detect all known genotypes, and the emergence of new genotypes of these viruses for case management in public health control activities.

Erythrovirus B19 (B19V) in patients with acute febrile illness suspected of arboviruses in Mato Grosso do Sul, Brazil

“…There are three recognized genotypes of B19V (1, 2, and 3), segregated into subtypes 1a, 1b, 2, 3a, and 3b [4,[33][34][35]. All three genotypes have been reported in both symptomatic and asymptomatic persons, and no association has been established between genotype and clinical manifestations [36][37][38][39][40][41]. Genotype 1 is the most prevalent in the world.…”

“…There was a greater number of cases in 2017 and 2018, having decreased in 2019, rising again in 2020, and decreasing again in 2021 and 2022, with a positivity percentage of 20.7%, 13.75%, 8.8 %, 18.3%, 2.3% and 3.4% respectively (Table 1; Figures 3 and 4A). However, further studies are needed over a longer period, since the cycle can last from 4 to 5 years [46]. Additionally, as three years of the study overlapped with the COVID-19 pandemic, this may also have influenced the dynamics of circulation and transmission of B19V.…”

Encephalopathy Caused by Human Parvovirus B19 Genotype 1 Associated With Haemophilus influenzae Meningitis in a Newborn

“…The Parvovirus B19 complete genome was recovered from the CSF and the genotypic showed, through phylogeny, sequences similar to B19 genotype 1. Genotype 1 is more prevalent in most parts of the world including Brazil [12,13], [46][47][48][49][50], showing a close relationship with the American sequence (Genebank #FJ591158). The other Brazilian sequences available in the reconstructions were relatively distant, suggesting a distant ancestor.…”

Encephalopathy Caused by Human Parvovirus B19 Genotype 1 Associated with Haemophilus influenzae Meningitis in a Newborn

“…The Parvovirus B19 complete genome was recovered from the CSF and the genotypic showed, through phylogeny, sequences similar to B19 genotype 1. Genotype 1 is more prevalent in most parts of the world including Brazil [ 12 , 13 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ], showing a close relationship with the Netherlands sequence (Genebank #JN211136). The other Brazilian sequences available in the reconstructions were relatively distant, suggesting a distant ancestor.…”