Experimental burns: Comparison between silver sulfadiazine and photobiomodulation

Abstract

Objective: To analyze morphological characteristics and organization of the collagen fibers of third degree burns from scalding compared to laser therapy and silver sulfadiazine, the latter considered as the gold standard. Method: Were selected 12 animals (Rattus norvegicus) also divided into three groups (control group [CG] -untreated burns; sulfadiazine group [SG] -burns were treated with silver sulfadiazine at 1%; laser group [LG] -burns were treated with photobiomodulation). The scald burns were carried out by using PVC mold, and the material collected on the 14 th day after burn was prepared for morphological and optical retardation analysis for evaluation of inflammatory infiltrates and collagen organization, respectively. Results: On the 14 th day, the laser and sulfadiazine groups had mild inflammatory response, while the control group showed an intense inflammatory process, with statistical significance between laser and control groups, but not between sulfadiazine and control groups. Laser and sulfadiazine groups no longer had granulation tissue, opposite to what was seen in the control group. The presence of hair follicles and ulcer did not significantly differ between groups. The optical retardation of collagen fibers was higher in sulfadiazine group, followed by laser and control groups. As for systemic effect, we were able to identify it by simply analyzing the presence or absence of granulation tissue. Conclusion: Morphologically, the laser or silver sulfadiazine treatments were similar and both provided better organization of collagen fibers in relation to the untreated group. However, the sulfadiazine group modulated the deposition of collagen fibers more efficiently than the laser group.

Effects and parameters of the photobiomodulation in experimental models of third-degree burn: systematic reviewanalysis

“…Of these, 1164 were excluded for not meeting the inclusion criteria of this systematic review: repeated study (n = 5), in vitro study (n = 120), clinical study (n = 62), systematic review (n = 76), ab-stract only (n = 47), it is not of third-degree burn (n = 854). Finally, 17 studies [4,[10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] in which diverse treatment pa-rameters of injuries were assessed were included for critical evaluation of the effectiveness of PBMT in third-degree burn. The 17 selected studies totaled a sample of 845 animals and showed some common characteristics, namely the use of adult male Wistar rat with a mean weight of 278.5 g as an experimental model and the dorsal region of the animals as the region where burns were performed in all (100%) studies.…”

“…Burns are the most extensive forms of soft tissue injuries, occasionally resulting in extensive and deep wounds and death. Burns can lead to severe mental and emotional distress, because of excessive scarring and skin contractures [4].…”

“…Thus, it has been extensively researched, particularly re-garding the factors that could delay or hinder the healing pro-cess [5]. Third-degree burns have been a major focus of re-search and investigation, searching for new treatment methods in order to improve the care of burn patients and also provide greater speed for a satisfactory result without major functional and esthetic sequelae [4].…”

The photobiomodulation (658, 830 and 904nm) on wound healing in histomorphometric analysis

“…The collagen deposition observed in this study was related to type III collagen. In fact, we analyzed the samples on the 14-2021876820 th day after the operative procedure, at which time the collagen type I deposition was not yet observed [37]. Furthermore, a better organization of the collagen fibers implies a better cicatricial process [38].…”

Light transmittance of the periodontium

“…This process results in beneficial therapeutic outcomes including but not limited to the alleviation of pain or inflammation, immunomodulation, and promotion of wound healing and tissue regeneration.^ [1]. PBM therapy was found to have multiple clinical benefits, which includes reducing pain [2][3][4][5][6][7], improving wound healing [8][9][10][11], accelerating bone healing [12][13][14][15][16][17], promoting osteointegration [18], facilitating orthodontic tooth movement [19][20][21][22][23][24][25][26], stimulating bone formation [27][28][29][30][31], and improving periodontal tissue repair and decreasing inflammation and root resorption [23,26,32]. The light sources were laser or a light-emitting diode (LED).…”