Recovery of motor function in rats with complete spinal cord injury following implantation of collagen/silk fibroin scaffold combined with human umbilical cord-mesenchymal stem cells

Deng, Wu-Sheng; Liu, Xiao‐Yin; Ma, Ke; Liang, Bing; Liu, Yingfu; Wang, Ren‐Jie; Chen, Xuyi; Zhang, Sai

doi:10.1590/1806-9282.20200697

Cited by 7 publications

(6 citation statements)

References 24 publications

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“…For general histopathological examination, histological slices (7 µm thick) were stained with hematoxylin and eosin stain (H&E). Modified Bielschowsky’s silver stain [ 88 ] and Luxol fast blue stain [ 89 ] were used for the detection of spinal cord myelination. The Cresyl violet stain was used to stain the neuronal cells and Nissl granules.…”

Section: Methodsmentioning

confidence: 99%

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model

Metwally

Al-Abbadi

Hashem

et al. 2022

IJMS

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Calpain activation has been implicated in various pathologies, including neurodegeneration. Thus, calpain inhibition could effectively prevent spinal cord injury (SCI) associated with neurodegeneration. In the current study, a dog SCI model was used to evaluate the therapeutic potential of a selective calpain inhibitor (PD150606) in combination with methylprednisolone sodium succinate (MPSS) as an anti-inflammatory drug. SCI was experimentally induced in sixteen mongrel dogs through an epidural balloon compression technique. The dogs were allocated randomly into four groups: control, MPSS, PD150606, and MPSS+PD150606. Clinical evaluation, serum biochemical, somatosensory evoked potentials, histopathological, and immunoblotting analyses were performed to assess treated dogs during the study. The current findings revealed that the combined administration of MPSS+PD150606 demonstrated considerably lower neuronal loss and microglial cell infiltration than the other groups, with a significant improvement in the locomotor score. The increased levels of inflammatory markers (GFAP and CD11) and calcium-binding proteins (Iba1 and S100) were significantly reduced in the combination group and to a lesser extent in MPSS or PD150606 treatment alone. Interestingly, the combined treatment effectively inhibited the calcium-induced cleavage of p35, limited cdk5 activation, and inhibited tau phosphorylation. These results suggest that early MPSS+PD150606 therapy after acute SCI may prevent subsequent neurodegeneration via calpain inhibition.

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Section: Methodsmentioning

confidence: 99%

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model

Metwally

Al-Abbadi

Hashem

et al. 2022

IJMS

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“…The International Society for Cell Therapy has put forward a general standard for MSCs: (1) under standard culture conditions, MSCs can adhere and grow on tissue culture vessels, that is, they must have viscoplasticity [ 12 ]; (2) MSCs must be able to express specific surface markers such as CD105, CD90, and CD34 [ 13 ]; (3) MSCs have the ability of osteogenic and adipogenic differentiation in vitro. This standard provides identification basis for preclinical and clinical studies of MSCs [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Human Adipose Derived Mesenchymal Stem Cells in Promoting Skin Wound Healing

Zhou

Wang

Yao³

et al. 2022

Journal of Healthcare Engineering

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Background. The aim of this pilot clinical study is to evaluate the efficacy of human adipose derived mesenchymal stem cells (HAMSCs) treatment for the wound healing with patients. Methods. This study was a clinical trial to investigate the efficacy of human adipose derived mesenchymal stem cells treatment for the wound healing with patients. 346 patients with skin wounds attending the central hospital of Yue Yang were enrolled in the study, setting in the period from January 2016 to January 2021. Patients were randomly allocated into two groups: experimental group received treatment with human adipose derived mesenchymal stem cells for each 10 cm2 of wound and control group received conventional dressing with normal saline for each 10 cm2 of wound. Results. No adverse events were recorded during the period of treatment. The granulation tissue coverage rate and thickness of granulation tissue after 10 days of treatment in experimental group were significantly improved compared with control group. Furthermore, the occurrence of bleeding of wound and suppurative wounds between two groups had significant difference ( P < 0.05 ). Conclusion. The data in this pilot study indicated that human adipose derived mesenchymal stem cells may be a safe and effective alternative therapy for wound healing. Moreover, larger, placebo-controlled, perspective studies are necessity to evaluate the efficacy and safety of human adipose derived mesenchymal stem cells treatment for wound healing patients.

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“…Traumatic spinal cord injury (TSCI) is a devastating central nervous system disease and can be divided into primary injury (initial mechanical injury) and secondary injury, including ischemia, oxidative stress, axonal degeneration, and cell death triggered by inflammatory processes [ 92 ]. The involvement of SF in post-SCI has been demonstrated in several studies [ 71 , 93 , 94 ]. In vitro culture of human amniotic epithelial cells (AECs) implanted with SF scaffolds showed active proliferation and migration, which improved motor function after SCI [ 95 ].…”

Section: Potential Roles Of Sf Hydrogels In Neurological Diseasesmentioning

confidence: 98%

Silk Fibroin Hydrogels Could Be Therapeutic Biomaterials for Neurological Diseases

Yang

Huang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Silk fibroin, a natural macromolecular protein without physiological activity, has been widely used in different fields, such as the regeneration of bones, cartilage, nerves, and other tissues. Due to irrevocable neuronal injury, the treatment and prognosis of neurological diseases need to be investigated. Despite attempts to propel neuroprotective therapeutic approaches, numerous attempts to translate effective therapies for brain disease have been largely unsuccessful. As a good candidate for biomedical applications, hydrogels based on silk fibroin effectively amplify their advantages. The ability of nerve tissue regeneration, inflammation regulation, the slow release of drugs, antioxidative stress, regulation of cell death, and hemostasis could lead to a new approach to treating neurological disorders. In this review, we introduced the preparation of SF hydrogels and then delineated the probable mechanism of silk fibroin in the treatment of neurological diseases. Finally, we showed the application of silk fibroin in neurological diseases.

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Recovery of motor function in rats with complete spinal cord injury following implantation of collagen/silk fibroin scaffold combined with human umbilical cord-mesenchymal stem cells

Cited by 7 publications

References 24 publications

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model

Efficacy of Human Adipose Derived Mesenchymal Stem Cells in Promoting Skin Wound Healing

Silk Fibroin Hydrogels Could Be Therapeutic Biomaterials for Neurological Diseases

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