Probiotic Lactobacilli Improved Growth Performance and Attenuated Salmonella Typhimurium Infection Via Jak/Stat Signaling in Broilers

Shi, Hanyi; Deng, Xuejuan; Deng, Qingqing; Liu, Z; Liu, N

doi:10.1590/1806-9061-2020-1328

Cited by 6 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, the pathogen loads in the organs were declined with the addition of the probiotic DPP8. This was supported by the literature that probiotics lowered the intestinal and faecal Salmonella counts in chickens or mice (Upadhaya et al 2016;Yin et al 2018;Adhikari et al 2019;Shi et al 2021). Inconsistently though, the spleen exhibited the greatest Salmonella load, but the probiotics did not decrease the pathogen load in the chicken viscera (Adhikari et al 2019).…”

Section: Discussionsupporting

confidence: 57%

“…It is necessary to confirm a specific probiotic strain that has some specific features through extensive and long-term clinical studies. In a previous study, the L. plantarum DPP8 strain has been reported to be capable of attenuating an S. Typhimurium infection by inhibiting Jak2/3 and Stat3/4/5/6 in chickens (Shi et al 2021); however, further studies are needed to confirm its efficacy and action mechanism in farm animals. Therefore, with a different experimental design than the previous study by Shi et al (2021), the present study aimed to further test the efficacy of probiotic DPP8 on an S. Typhimurium infection, Jak2, and Stat1/3 in rabbits.…”

mentioning

confidence: 98%

See 1 more Smart Citation

Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits

Wen¹,

Liu²,

Ding³

et al. 2022

Vet. Med. - Czech

View full text Add to dashboard Cite

This study aimed to investigate the effect of a Lactobacillus plantarum DPP8 supplementation on the growth performance, pathogenic invasion, inflammation and pathogen resistance signal pathway in rabbits infected with Salmonella Typhimurium (S. Typhimurium). The treatments included a negative control, a positive control with an S. Typhimurium infection and a positive control plus DPP8 at 106, 108 or 1010 cfu/kg of diet using 300 weaned rabbits. The results showed that supplementing with DPP8 improved (P < 0.05) the feed intake, body weight gain and feed efficiency compared to the positive control. Also, DPP8 decreased (P < 0.05) the S. Typhimurium colonisation and translocation, serum IL-1β, IL-6 and TNF-α, and intestinal mucosa mRNA expressions of the inflammatory mediators Janus kinase (Jak) 2, the signal transducer and activator of transcription (Stat) 1 and 3 at 7 and 14 d post administration. The dose analysis of DPP8 showed linear increases (P ≤ 0.007) in the feed intake and body weight gain, but linear decreases (P ≤ 0.022) in the S. Typhimurium loads, IL-1β, IL-6 and Jak2. It is concluded that Lactobacillus plantarum DPP8 can be used as a supportive probiotic against an S. Typhimurium infection and it possibly plays a direct or indirect role in the downregulation of the Jak/Stat pathway in rabbits.

show abstract

Section: Discussionsupporting

confidence: 57%

mentioning

confidence: 98%

Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits

Wen¹,

Liu²,

Ding³

et al. 2022

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…In recent years, there has been growing interest in the vital link between the gut microbiota and the growth performance of broiler chickens. Dietary supplementation with some bioactive compounds, such as probiotics, enhanced the growth performance of broiler chickens by improving gut health [ 140 , 141 , 142 , 143 ]. Furthermore, the early intervention with some bioactive substances may provide early bacterial colonization in the gut of newly hatched chicken and improve the gut microbiota and, consequently, improve gut health and growth performance ( Table 2 ).…”

Section: Gut Microbiota and Growth Performancementioning

confidence: 99%

Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens

Shehata

Paswan

Attia

et al. 2021

Animals

View full text Add to dashboard Cite

The chicken gut is the habitat to trillions of microorganisms that affect physiological functions and immune status through metabolic activities and host interaction. Gut microbiota research previously focused on inflammation; however, it is now clear that these microbial communities play an essential role in maintaining normal homeostatic conditions by regulating the immune system. In addition, the microbiota helps reduce and prevent pathogen colonization of the gut via the mechanism of competitive exclusion and the synthesis of bactericidal molecules. Under commercial conditions, newly hatched chicks have access to feed after 36–72 h of hatching due to the hatch window and routine hatchery practices. This delay adversely affects the potential inoculation of the healthy microbiota and impairs the development and maturation of muscle, the immune system, and the gastrointestinal tract (GIT). Modulating the gut microbiota has been proposed as a potential strategy for improving host health and productivity and avoiding undesirable effects on gut health and the immune system. Using early-life programming via in ovo stimulation with probiotics and prebiotics, it may be possible to avoid selected metabolic disorders, poor immunity, and pathogen resistance, which the broiler industry now faces due to commercial hatching and selection pressures imposed by an increasingly demanding market.

show abstract

“…Inflammatory cascades are triggered via the activation of genes such as TIR domain-containing adaptor protein (TIRAP), IL-1 receptor-associated kinase (IRAK), receptor-interacting serine/threonine-protein kinase 2 (RIP), and NF-kappa-B essential modulator (NEMO) [ 5 , 6 ]. On the other hand, the Janus kinase/signal transduction and activator of transcription (JAK/STAT) signaling pathway is a significant component of the innate and adaptive immune systems that mediate cytokines, having a critical role in inflammatory diseases [ 7 ]. Different JAK/STAT system components act through association with various cytokines.…”

Section: Introductionmentioning

confidence: 99%

The effects of the probiotic cocktail on modulation of the NF-kB and JAK/STAT signaling pathways involved in the inflammatory response in bowel disease model

et al. 2022

View full text Add to dashboard Cite

Background Probiotics positively affect inflammatory responses, in part, through Janus kinase/signal transduction and activator of transcription (JAK/STAT) and inflammatory signaling pathways. To evaluate the precise effects of probiotics as protective treatment, we aimed to investigate the effectiveness of Lactobacillus spp., Bifidobacterium spp., and a mixture of these probiotics in modulating the JAK/STAT and inflammatory signaling pathways. Methods A quantitative real-time polymerase chain reaction (qPCR) assay was used to analyze the expression of JAK/STAT and inflammatory genes (TIRAP, IRAK4, NEMO, and RIP) following HT-29 cell line treatment with sonicated pathogens Lactobacillus spp., Bifidobacterium spp., and a mixed cocktail. A cytokine assay was also used to evaluate the IL-6 and IL-1β production following the probiotic treatment. Results The probiotic cocktail downregulated the JAK genes and TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway compared to sonicate pathogen treatment cells. The expression of STAT genes was variable following probiotic treatment. The IL-6 and IL-1β production decreased after probiotic treatment. Conclusions Our probiotic cocktail showed anti-inflammatory effects on HT-29 cells by modulating JAK/STAT and NF-kB pathways. Therefore, Lactobacillus spp. and Bifidobacterium spp. probiotics as nutritional supplements may reduce inflammation-associated diseases such as inflammatory bowel disease (IBD).

show abstract

Probiotic Lactobacilli Improved Growth Performance and Attenuated Salmonella Typhimurium Infection Via Jak/Stat Signaling in Broilers

Cited by 6 publications

References 37 publications

Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits

Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits

Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens

The effects of the probiotic cocktail on modulation of the NF-kB and JAK/STAT signaling pathways involved in the inflammatory response in bowel disease model

Contact Info

Product

Resources

About