Effects of Dietary Energy Concentration on Reproductive Hormone Secretion and Gene Expression in the Hypothalamus-Pituitary-Gonad Axis in Laying Geese

Liu, Z; Xue, Jiajia; Luo, Yuheng; Wang, Q; Zhong, Huan; Liang, Mingkun; Wang, C

doi:10.1590/1806-9061-2019-1017

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…After feeding Zhedong White Geese with phytoestrogen daidzein to examine its effect on mRNA levels in the HPG axis, ESR2 was significantly found in the ovary where estrogen is mainly localized ( Zhao et al, 2013 ). Again, when laying geese were fed with dietary energy concentration, estrogen mRNA levels were higher in the ovaries of animals fed with a sufficient energy diet than those fed with deficient energy diets ( Liu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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Our previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. In this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms ( SNPs ) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. A total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly ( P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 3-20G>A showed that the haplotypes were significantly associated with egg weight. Higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal ( HPG ) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly ( P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly ( P < 0.05) higher in the infundibulum and magnum of laying and non-laying ducks respectively. This study provides a molecular marker for selecting Leizhou black ducks for egg production. In addition, it offers theoretical knowledge for studying the related biological functions of the ESR2 gene at the cellular level.

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Section: Discussionmentioning

confidence: 99%

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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“…A high circulating IGF-1 concentration in laying hens is related to a high egg weight [16], egg production rate [17], and feed efficiency [18]. Liu et al [19] reported that the increase of serum IGF-1 concentration in geese corresponded to the improvement of egg weight and egg production rate and the reduction of feed conversion ratio. Sabry et al [20] observed that laying hens with higher serum IGF-1 concentration had a high egg production rate and egg mass and a low feed conversion ratio.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation of herbal active ingredients promoting insulin-like growth factor-1 secretion on production performance, egg quality, blood hematology, and excreta gas emission in laying hens

2021

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Objective: The purpose of this study was to evaluate the effects of supplementing herbal active ingredients (YGF251) which can promote the secretion of insulin-like growth factor-1 (IGF-1) in the diet on production performance, egg quality, blood hematology, and excreta gas emission in laying hens. A c c e p t e d A r t i c l eMethods: A total of 288 ISA Brown (41-week-old) laying hens with an initial body weight of 1.83 ± 0.68 kg were randomly assigned to 1 of 4 dietary treatments in a randomized block design based on body weight.Each treatment had replicate cages having 6 adjacent cages per replicate (hens are kept in cages alone). The experimental period was 35 days. Dietary treatments were based on the corn-soybean meal-wheat-based basal diet and supplemented with 0.00, 0.05, 0.10, or 0.15 % YGF251.Results: The linear increased egg weight in weeks 1 to 5 (P < 0.05), egg mass in week 1 (P < 0.05) and weeks 1 to 5 (P < 0.05), egg strength on day 7 (P < 0.05), 21 (P < 0.01), and 35 (P < 0.01), eggshell thickness on day 21 (P < 0.05) and 35 (P < 0.01), haugh unit on day 21 (P < 0.01) and 35 (P < 0.05), serum IGF-1 concentration on day 21 (P < 0.05) and 35 (P < 0.01), and serum total protein concentration on day 35 (P < 0.05) were observed with the supplementing YGF251 increased in the diet, while feed conversion ratio in weeks 1 to 5 (P < 0.05) and excreta ammonia emission (P < 0.01) decreased linearly with the dose of YGF251 increased.Conclusion: Dietary supplementation of YGF251 positively affected the production performance and egg quality of laying hens through increasing serum IGF-1 concentration in a dose-dependent manner.Moreover, YGF251 supplementation improved barn environment by reducing excreta noxious gas emission.

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“…After feeding Zhedong White Geese with phytoestrogen daidzein to examine its effect on mRNA levels in the HPG axis, ESR2 was significantly found in the ovary where estrogen is mainly localized (66). Again, when laying geese were fed with dietary energy concentration, estrogen mRNA levels were higher in the ovaries of animals fed with a sufficient energy diet than those fed with deficient energy diets (67).…”

Section: Esr2 Distribution Pattern In the Hypothalamic-pituitary-gonadal (Hpg) Axis Of Laying And Non-laying Leizhou Black Ducksmentioning

confidence: 98%

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Asiamah

Liu

et al. 2021

Preprint

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BackgroundOur previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. MethodIn this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms (SNPs) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four (4) each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. ResultsA total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly (P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 320G>A showed that the haplotypes were significantly associated with egg weight where higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal (HPG) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly (P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly (P < 0.05) higher in the infundibulum and magnum of laying and nonlaying ducks respectively. ConclusionThis study provides molecular marker for selecting Leizhou black ducks for egg production and provides theoretical knowledge for the study of the related biological functions of the ESR2 gene at the cellular level.

show abstract

Effects of Dietary Energy Concentration on Reproductive Hormone Secretion and Gene Expression in the Hypothalamus-Pituitary-Gonad Axis in Laying Geese

Cited by 3 publications

References 22 publications

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Effects of dietary supplementation of herbal active ingredients promoting insulin-like growth factor-1 secretion on production performance, egg quality, blood hematology, and excreta gas emission in laying hens

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

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