The Single Nucleotide Polymorphisms of Myostatin Gene and Their Associations With Growth and Carcass Traits in Daheng Broiler

Xx, Zhang; Ran, JS; Lian, Ting; Zq, Li; Yang, Chaowu; Xs, Jiang; Du, Huarui; Cui, ZF; Yp, Liu

doi:10.1590/1806-9061-2018-0808

Cited by 11 publications

(10 citation statements)

References 30 publications

(26 reference statements)

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“…A recent study showed that in Nigerian indigenous turkey, MSTN gene exon 1 had a moderate to a high degree of polymorphism (Fijabi et al, 2020). Additionally, Tanjung et al (2019) Other studies demonstrated that MSTN polymorphisms have positive effect on the growth trait in broiler, geese and fish (Aboukila et al, 2021;Smołucha et al, 2019;Zhang et al, 2019). Although the results did not show a significant difference between between the high and low markting weights of turkey.…”

Section: Discussionmentioning

confidence: 91%

Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey

Basha¹,

Naby²,

Ibrahim³

et al. 2022

AAVS

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This study was conducted to evaluate the relationship between variant body weight and carcass traits of Black Bronze turkey with the single nucleotide polymorphism in the coding region of the MSTN gene and IGF-2 (3'-UTR), using DNA sequencing. Fifty turkeys were categorized into high and low body weight. Blood samples were collected from each bird for DNA extraction. Four birds were selected randomly/group to be slaughtered for carcass traits. In MSTN, only six non-synonymous SNPs were recorded leading to four amino acid substitution in exon 1 and 2 amino acids substitution in exon 2. In low body weight, only one non-synonymous mutation was recorded at exon 2. The 3'UTR-IGF-2 sequence showed 14 SNPs in high body weight and three SNPs in low body weight. The carcass weight percentage didn't change significantly for high or low body weight. While carcass cuts percentage increased in high body weight birds compared to low weight. We conclude that no association was present between the SNPs recorded neither at MSTN coding region nor at IGF-2-3'UTR and the body weight or carcass traits. Further studies are needed to associate these SNPs with different growth stages of turkey. Keywords | Black Bronze turkey; MSTN; IGF-2; SNPs; Body weight Heba abdo basHa 1 , Walaa slouma Hamouda abd el Naby 2* , samya eriaN ibraHim 3 , abeer Fikry elNaHas 2

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Section: Discussionmentioning

confidence: 91%

Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey

Basha¹,

Naby²,

Ibrahim³

et al. 2022

AAVS

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“…Our results indicated that the exotic turkey which is a commercial broiler turkey is more polymorphic in the exonic region compared to their local counterpart although no variation or loss of function mutation in MSTN gene has been reported to influence growth performance in turkey. Several authors have reported different polymorphisms in the gene with some of them associated with growth performance in broiler chickens (Bhattacharya and Chatterjee, 2013;Dushyanth et al, 2016;Zhang et al, 2019). Hu et al (2013) has also reported the occurrence of Single nucleotide polymorphisms in the upstream regulatory region that alter the expression of myostatin gene in chicken.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Single Nucleotide Polymorphism and Genetic Diversity at the Myostatin gene Locus in Indigenous and Locally Adapted Exotic Turkey breeds in Nigeria

Ilori¹,

Isong²,

Oghate³

et al. 2022

Nig. J. Biotechnol

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This research was carried out to characterize single nucleotide polymorphism at the myostatin gene locus in indigenous and Nigerian locally adapted exotic turkeys. A total of 220-day-old poults comprising 120 local and 100 locally adapted exotic turkeys were sourced from reputable hatchery and were managed for 20 weeks under intensive management system. Blood samples were collected from 70 turkeys each form the two breeds via the brachial vein into Ethylene Diamine Tetra Acetic Acid (EDTA) bottles for DNA extraction and amplification of target regions using commercially available kits when the birds were 8 weeks. Genomic regions containing exon 1 and 2 of MSTN gene including their surrounding introns were sequenced and analyzed using BioEdit, Codon Code Aligner, DnaSP and MEGA software. Codon-based test was also performed to estimate the ratio of non-synonymous substitutions (dN) to synonymous substitutions (dS). Results from our study showed that both local and exotic turkey breeds had one non-synonymous single nucleotide polymorphism (SNP) (248 G>A) in exon 1 while SNP variant 333 G>A was detected only in exon 2 of exotic turkey. In intron 1, a total of 3 SNPs in local turkey and 2 SNPs in exotic turkey were detected. Intron 2 also revealed 4 and 3 SNPs in local and exotic turkeys respectively. Genetic diversity indices showed that local turkey had higher haplotype diversity at intron 1 (75 %) and intron 2 (84 %) while haplotype diversity of 20 % was estimated at exon 2 in exotic turkey. Our Codon-based test of selection showed dN/dS ratio of <1 (purifying selection) at G248A SNP loci, suggested a possible role of this non-synonymous SNP variant on growth performance. We recommend a population-based study to investigate the effect of this non-synonymous SNP G248A on growth and morpho-structural traits for breed improvement and conservation of our locally adapted turkey populations.

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“…The associations of the SNPs and haplotypes of CD1B gene with IgY and ND antibody titer were performed by General Linier Model (GLM) procedure using SAS 9.2 software (SAS Institute, Cary, NC, USA) and least means square values (x ̅ ) for genotypes and haplotypes were compared by Duncan`s Multiple Range test (Zhang et al, 2019). Significant associations were declared when p<0.05.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

Al-Habib

Murtini

Gunawan

et al. 2020

Trop. Anim. Sci. J.

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The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G>A, c.562 T>A, c.588 A>G, and c.612 C>G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G>A and c.562 T>A SNPs were in Hardy Weinberg's equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A>G and c.612 C>G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e.

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The Single Nucleotide Polymorphisms of Myostatin Gene and Their Associations With Growth and Carcass Traits in Daheng Broiler

Cited by 11 publications

References 30 publications

Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey

Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey

Evaluation of Single Nucleotide Polymorphism and Genetic Diversity at the Myostatin gene Locus in Indigenous and Locally Adapted Exotic Turkey breeds in Nigeria

Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

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