2017
DOI: 10.1590/1806-9061-2016-0406
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Abstract: Aquaporin 2 (AQP2) is a small protein located in the collecting tubules of kidneys; it plays an important role in the concentration and production of urine. The aim of this study was to determine the expression level of the AQP2 gene in the kidney of broiler chickens after the administration of renal dose dopamine. Broiler chickens (25 days-old) were randomly divided into two groups (n=20 per group): intravenous administration of saline solution (control group) or renaldose dopamine (dopamine group). The expre… Show more

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Cited by 5 publications
(5 citation statements)
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“…The RT-qPCR was performed in quadruplex with Step One-Plus TM Real-Time PCR System (Applied Biosystems). The RT-qPCR reaction system was performed according to our previous study 50 , 51 . All the reaction mixture was normalized against the reference gene GAPDH.…”
Section: Methodsmentioning
confidence: 99%
“…The RT-qPCR was performed in quadruplex with Step One-Plus TM Real-Time PCR System (Applied Biosystems). The RT-qPCR reaction system was performed according to our previous study 50 , 51 . All the reaction mixture was normalized against the reference gene GAPDH.…”
Section: Methodsmentioning
confidence: 99%
“…The qRT-PCR was run in quadruplex with Step One-Plus TM Real-Time PCR System (Applied Biosystems). The reaction mixture specifications were according to our previous study ( Chang et al, 2017 ). All the reaction mixture was normalized against the reference gene GAPDH.…”
Section: Methodsmentioning
confidence: 99%
“…The presence of AQP2 in the kidney plays a role in water reabsorption [ 43 ]. Aquaporin 2 is a small protein located in the collecting tubules of the kidney and plays an important role in the concentration and production of urine [ 44 ]. The presence of AQP might affect the loss of body fluid of chickens because when broiler chickens experience heat stress, they might lose more water (through gasping and urination) and result in increased water consumption to compensate for water loss and to increase heat dissipation capacity.…”
Section: Discussionmentioning
confidence: 99%