Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Abstract

The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

“…Recently, research workers isolated S . Newport from chicken meat [28,29]. Report of S. Newport in a poultry farm and processing environment is a serious issue, and rapid rise of MDR Salmonella serovar Newport isolates over the past decade as important causes of human Salmonellosis [30].…”

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

“…At the same time, its ability to form biofilms stands out; this hinders its control in the food industry [ 16 ]. In this condition, microorganisms have different responses to heat treatments, biocides, and antimicrobials, and are therefore a constant source of contamination [ 17 , 18 ].…”

Salmonella Minnesota Emergence in Poultry Production: Danger to Public Health?

“…At the same time, among the hypotheses that may explain S. Minnesota persistence in the environment, its ability to form bio lms stands out, which hinders its control in the food industry [46]. In this condition microorganisms have different responses to heat treatments, biocides and antimicrobials, and are therefore a constant source of contamination [18,27,40].…”