Evaluation of Incubation Yield, Vaccine Response, and Performance of Broilers Submitted to In-Ovo Vaccination at Different Embryonic Ages

Fernandes, Jim; Prokoski, Karen; Oliveira, B de; Oro, CS; Oro, PJ; Fernandes, Nlm

doi:10.1590/1806-9061-2015-0216

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…It is important to have effective methods of mass vaccination because flock sizes of commercial poultry contain thousands of birds. In-ovo vaccines are a mass vaccination strategy that can reduce stressful post-hatching procedures [ 15 ]. In-ovo vaccines are currently approved for use in poultry flocks for different diseases, e.g., Marek’s disease.…”

Section: Introductionmentioning

confidence: 99%

“…Typically, the in-ovo vaccine is injected into the amniotic sac and the amnion fluid containing the vaccine antigen gets swallowed by the embryo, giving way to “oral vaccination” at an early stage [ 16 ]. In-ovo vaccination induces early immunity with less interference from maternal antibodies [ 15 ]. Early immunity can also aid in significantly reducing the inflammatory response upon future encounters with the antigen [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of a nanoparticle vaccine administered in-ovo against Salmonella in broilers

et al. 2021

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Salmonella is a zoonotic pathogen that persists in poultry. Salmonella vaccines that can be delivered in-ovo can be cost-effective and can decrease Salmonella load in poultry. This study evaluates the efficacy of a Salmonella chitosan-nanoparticle (CNP) vaccine, administered in-ovo, in broilers. CNP vaccine was synthesized with Salmonella Enteritidis (SE) outer-membrane-proteins (OMPs) and flagellin proteins. At embryonic-d18, one-hundred-thirty-six eggs were injected with 200μl PBS or 1000μg CNP into the amniotic cavity. At d1-of-age, 132 chicks were allocated in 6 pens/treatment with 11 chicks/pen. At d7, birds were orally challenged with 1×109 CFU/bird SE. At d1, 8h-post-challenge, d14, and d21, serum anti-SE-OMPs IgY were analyzed. At d14 and d21, cloacal swabs and bile anti-SE-OMPs IgA, CD4+/CD8+-T-cell ratios, and ceca SE loads were analyzed. At d21, cecal tonsil IL-1β, IL-10, and iNOS mRNA were analyzed. Body-weight-gain (BWG) and feed-conversion-ratio (FCR) were recorded weekly. Data were analyzed by Student’s t-test at P<0.05. There were no significant differences in BWG or FCR between vaccinated birds compared to control. At d1, CNP-vaccinated birds had 5.62% greater levels (P<0.05) of anti-SE-OMPs IgY, compared to control. At 8h-post-challenge, CNP-vaccinated birds had 6.39% greater levels (P<0.05) of anti-SE-OMPs IgY, compared to control. At 2wk-post-challenge, CNP-vaccinated birds had 7.34% lower levels (P<0.05) of anti-SE-OMPs IgY, compared to control. At 1wk-post-challenge, CNP-vaccinated birds had 15.30% greater levels (P<0.05) of bile anti-SE-OMPs IgA, compared to control. At d14 and d21, CNP-vaccinated birds had 0.62 and 0.85 Log10 CFU/g, decreased SE ceca load (P<0.05), respectively, compared to control. There were no significant differences in CD4+/CD8+-T-cell ratios between vaccinated birds compared to control. There were no significant differences in IL-1β, IL-10, iNOS mRNA between vaccinated birds compared to control. Findings demonstrate that the in-ovo administration of CNP vaccine can induce an antigen-specific immune response against SE and can decrease SE cecal load in broilers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of a nanoparticle vaccine administered in-ovo against Salmonella in broilers

et al. 2021

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“…One study, reported that vaccination of 10–12 day-old embryos with herpes virus of turkeys (HVT) led to pronounced lesions and embryonic deaths, while vaccination on days 16 did not cause detectable lesions [ 78 ]. Embryonic age at vaccination has also been shown to be correlated with antibody titers [ 79 ]. Maternal antibody titers actually increase after the typical age for in-ovo vaccinations and peak just after hatch [ 76 ].…”

Section: New Approaches For the Development Of Veterinary Vaccinesmentioning

confidence: 99%

Vaccines as alternatives to antibiotics for food producing animals. Part 2: new approaches and potential solutions

Hoelzer

Bielke²,

Blake

et al. 2018

Vet Res

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Vaccines and other alternative products are central to the future success of animal agriculture because they can help minimize the need for antibiotics by preventing and controlling infectious diseases in animal populations. To assess scientific advancements related to alternatives to antibiotics and provide actionable strategies to support their development, the United States Department of Agriculture, with support from the World Organisation for Animal Health, organized the second International Symposium on Alternatives to Antibiotics. It focused on six key areas: vaccines; microbial-derived products; non-nutritive phytochemicals; immune-related products; chemicals, enzymes, and innovative drugs; and regulatory pathways to enable the development and licensure of alternatives to antibiotics. This article, the second part in a two-part series, highlights new approaches and potential solutions for the development of vaccines as alternatives to antibiotics in food producing animals; opportunities, challenges and needs for the development of such vaccines are discussed in the first part of this series. As discussed in part 1 of this manuscript, many current vaccines fall short of ideal vaccines in one or more respects. Promising breakthroughs to overcome these limitations include new biotechnology techniques, new oral vaccine approaches, novel adjuvants, new delivery strategies based on bacterial spores, and live recombinant vectors; they also include new vaccination strategies in-ovo, and strategies that simultaneously protect against multiple pathogens. However, translating this research into commercial vaccines that effectively reduce the need for antibiotics will require close collaboration among stakeholders, for instance through public–private partnerships. Targeted research and development investments and concerted efforts by all affected are needed to realize the potential of vaccines to improve animal health, safeguard agricultural productivity, and reduce antibiotic consumption and resulting resistance risks.

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Evaluation of probiotic and glutamine in ovo on broilers challenged with Salmonella Heidelberg

Oro¹,

Ouros²,

Ribeiro³

et al. 2023

Journal of Applied Poultry Research

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Evaluation of Incubation Yield, Vaccine Response, and Performance of Broilers Submitted to In-Ovo Vaccination at Different Embryonic Ages

Cited by 4 publications

References 7 publications

Efficacy of a nanoparticle vaccine administered in-ovo against Salmonella in broilers

Efficacy of a nanoparticle vaccine administered in-ovo against Salmonella in broilers

Vaccines as alternatives to antibiotics for food producing animals. Part 2: new approaches and potential solutions

Evaluation of probiotic and glutamine in ovo on broilers challenged with Salmonella Heidelberg

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