A lima bean core collection based on molecular markers

Abstract

Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire germplasm bank (1.605 and 0.713, respectively), indicating a higher polymorphism of the representative cultivars in relation to the entire collection. The accessions selected for the core collection may be used in future studies of genome association as well as in genetic crosses in breeding programs aimed at developing improved cultivars with high genetic diversity which can meet current and future market needs.

The Brazilian lima bean landraces: conservation and breeding

“…(2017) also found a high genetic diversity (0.60) in 14 accessions of lima bean from Brazil, higher than those reported for other regions of America and similar to those of wild populations of the Yucatán Peninsula, in Mexico, using 10 SSRs markers. Gomes et al (2020) analyzed the genetic diversity of 153 accessions of lima bean to build a nuclear collection, using 11 SSRs markers, and found that 34 accessions were representative of the entire germplasm bank, with greater genetic diversity (0.81) compared to the entire bank (0.71).…”

“…According to Baudoin (1988), the projection of the stigma and stylus out of the perianth and the receptivity of the stigma during many hours can be responsible for these high crossing rates. Gomes et al (2020), analyzed the genetic diversity of accessions of lima bean to build a nuclear collection, using SSRs markers, and also found that the groups in which the studied accessions were separated did not re ect their geographic origins. Guimarães et al (2007), through a dendrogram constructed from 22 lima bean accessions analyzed through RAPD markers, formed two main groups, whose accessions were not divided according to their origin.…”

“…Additionally, Chacón reported that the geographical localization of Brazil is favorable to the Andean and Mesoamerican germplasms. However, there are few studies about lima bean in Brazil, mainly concentrated on genetic resource conservation (Brito-Silva et al 2015;Penha et al 2017;Silva et al 2017;Silva et al 2019;Gomes et al 2020). It is necessary to expand the knowledge of genetic characterization of lima bean in Brazil.…”

Feijão-fava: doenças virais e fúngicas

“…O feijão-fava apresenta elevado potencial de cultivo, diversidade genética e adaptação a diferentes condições climáticas (VIEIRA 1992;LONG et al 2014;GOMES et al 2020). No Brasil, destaca-se como espécie de subsistência, sendo produzida em…”

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INTRODUÇÃODentre os feijões cultivados para o consumo humano, o feijão-fava (Phaseolus lunatus L.) é representativo nos cenários agrícola, econômico e cultural para a população da região Nordeste do Brasil, uma vez que é apresentado como alternativa da sustentabilidade da produção de alimentos (CHEL-GUERRERO et al 2012;MOSES et al 2012).O feijão-fava apresenta elevado potencial de cultivo, diversidade genética e adaptação a diferentes condições climáticas (VIEIRA 1992;LONG et al 2014;GOMES et al 2020). No Brasil, destaca-se como espécie de subsistência, sendo produzida em

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