Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cheng, Lifeng; Wang, Qiming; Feng, Xiaoli; Duan, Shengwen; Yang, Qi; Zheng, Ke; Liu, Zhiyuan; Liu, Zhengchu; Peng, Yu

doi:10.1590/1678-992x-2017-0108

Cited by 20 publications

(22 citation statements)

References 21 publications

(21 reference statements)

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“…Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms [10][11][12][13] . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting [14][15][16][17][18] along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 .…”

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confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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Jute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute retting water samples in Bangladesh. Higher pectinolytic bacterial isolates were predominant in the later stage of jute retting. Out of these, 168 isolates have been screened by both semi-quantitative and quantitative pectinase, xylanase and cellulase enzyme assay. Among them, 144 isolates have been selected on the basis of extra cellular enzyme activity of these three enzymes. 16 s ribosomal gene sequencing analysis identified 2 phyla-Firmicutis (80.55%) and Proteobacteria (19.45%). To check the synergistic and antagonistic effect 10 selected isolates were tested in 167 different combinations. Three best combinations were identified that lowered retting period from 18-21 days to 10 days producing high quality fibre in both laboratory and field trial. This improved retting technology can be adopted in industrial scale for the production of quality jute fibre in a controlled condition in reduced water quantity without polluting the environment.Jute, second most important natural fibre after cotton, is cultivated in East Asia and some parts of Latin America 1-3 . Jute is a bast or phloem fibre in the bark of stems, cemented together by pectin and gummy substances 4 . Commercial extraction of jute fibre is water based microbiological retting where jute bundles are submerged into slow running river water and subjected to decomposition of pectin, hemicelluloses, and other mucilaginous substances 2,5 . In this process, pectin is depolymerized by pectinases, primarily comprising four enzymes: Polygalacturonase (PG), Pectin Lyase (PNL), Pectate lyase and Pectin esterase. However, PG 6 , and PNL 7 are primary retting enzymes. In addition, xylanase makes jute fibre softer by selective removal of non-fibrous hemicelluloses without affecting strength of cellulosic fibre. Pectinolytic microorganisms having xylanase activity devoid of cellulase activity is an additional beneficial aspect to improve fibre quality 8 . The quality of fibre is largely determined by the efficiency of retting process 1,2,9,10 and various factors are responsible for proper retting as well as improved fibre quality. Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms 10-13 . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting 14-18 along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 . As microorganisms are the ma...

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confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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“…Using the 16S/18S rRNA sequencing, we identified the changed bacterial and fungal abundance during the degumming of kenaf bast (0~ 150 h). In the fermentation liquid, the growth of Cytophagaceae and A series of Bacillus strains has been identified to be ramie-or kanef-degumming strains, like B. cereus hn1-1 [10], B. pumilus [7], B. licheniformis and B. subtilis [11] and B. tequilensis SV11-UV37 [6]. In addition, our previous study [7] showed that seven bacterial strains including B. pumilus, B.…”

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Insights on bio-degumming of kenaf bast based on metagenomic and proteomics

Duan

Cheng

Feng

et al. 2019

Preprint

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Background: Microbial play important roles in kanef-degumming. This study aims at identifying the key candidate microbial and proteins responsible for the degumming of kenaf bast ( Hibiscus cannabinus ). Kenaf bast was cut into pieces and immersed into microbial fermentation liquid collected from different sites. Fermentation liquid samples were collected at 0, 40, 110 and 150 h and then subjected to the 16S/18S rRNA sequencing analysis and isobaric tag for relative and absolute quantitation (iTRAQ) analysis. The microbial (bacterial and fungal) diversity and the differentially expressed proteins/peptides (DEPs) were identified. Results: With the prolonged degumming time, the weight loss rate increased, the bacterial diversity was decreased. [Weeksellaceae], Enterobacteriaceae and Moraxellaceae were rapidly increased at 0~40 h, and then decreased and were gradually replaced by Bacteroidaceae from 40 h to 150 h. Similarly, Chryseobacterium and Dysgonomonas were gradually increased at 0~110 h and then decreased; Acinetobacter and Lactococcus were increased at 0~40 h, followed by decrease. Bacteroides was the dominant genus at 150 h. 18S rRNA-seq showed the gradually decreased Wallemia_hederae and increased Codosiga_hollandica during degumming. iTRAQ data analysis showed Rds1, and pyruvate kinase I was decreased and increased in the kanef-degumming, respectively. Other DEPs of ferredoxin I, superoxide dismutase and aconitatehydratase were identified to be related to the Glyoxylate and dicarboxylate metabolism (ko00630). Conclusions : Bacteria including Chryseobacterium , Dysgonomonas , Acinetobacter , Lactococcus and Bacteroides and fungi like Wallemia_hederae and Codosiga_hollandica are key candidate microbial for kanef degumming.

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“…The screening of superior bacterial strains with the activity of pectate lyase, pectinase, hemicellulase and/or ligninase and the preservation of the natural fiber structure and mechanical properties is crucial for biological degumming [7][8][9]. A series of bacterial strains have been identified with strong ability of retting or degumming, like Bacillus cereus hn1-1 [10], B. pumilus [7], B. licheniformis and B. subtilis [11] and B. tequilensis SV11-UV37 [6]. Cheng et al [10] showed that the 10 h-degumming process by B. cereus hn1-1 produced a residual gum rate as low as 5% and the fiber rate as high as 76%.…”

Section: Introductionmentioning

confidence: 99%

“…A series of bacterial strains have been identified with strong ability of retting or degumming, like Bacillus cereus hn1-1 [10], B. pumilus [7], B. licheniformis and B. subtilis [11] and B. tequilensis SV11-UV37 [6]. Cheng et al [10] showed that the 10 h-degumming process by B. cereus hn1-1 produced a residual gum rate as low as 5% and the fiber rate as high as 76%. Mao et al [12] reported that the ramie retting could be completed within 56 h by using a microbia consortium RAMCD407 plus 0.2% NaOH, with 2.84% residual gum content and 5.2 cN/dtex breaking strength of the final fiber.…”

Section: Introductionmentioning

confidence: 99%

Insights on bio-degumming of kenaf bast based on metagenomic and proteomics

et al. 2020

Self Cite

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Background: Microbes play important roles in kanef-degumming. This study aims at identifying the key candidate microbes and proteins responsible for the degumming of kenaf bast (Hibiscus cannabinus). Kenaf bast was cut into pieces and immersed into microbia fermentation liquid collected from different sites. Fermentation liquid samples were collected at 0, 40, 110 and 150 h and then subjected to the 16S/18S rRNA sequencing analysis and isobaric tag for relative and absolute quantitation (iTRAQ) analysis. The microbial (bacterial and fungal) diversity and the differentially expressed proteins/peptides (DEPs) were identified. Results: With the prolonged degumming time, the weight loss rate increased, the bacterial diversity was decreased. [Weeksellaceae], Enterobacteriaceae and Moraxellaceae were rapidly increased at 0~40 h, and then decreased and were gradually replaced by Bacteroidaceae from 40 h to 150 h. Similarly, Chryseobacterium and Dysgonomonas were gradually increased at 0~110 h and then decreased; Acinetobacter and Lactococcus were increased at 0~40 h, followed by decrease. Bacteroides was the dominant genus at 150 h. Sequencing 18S rRNA-seq showed the gradually decreased Wallemia hederae and increased Codosiga hollandica during degumming. iTRAQ data analysis showed Rds1, and pyruvate kinase I was decreased and increased in the kanef-degumming, respectively. Other DEPs of ferredoxin I, superoxide dismutase and aconitatehydratase were identified to be related to the Glyoxylate and dicarboxylate metabolism (ko00630). Conclusions: Bacteria including Chryseobacterium, Dysgonomonas, Acinetobacter, Lactococcus and Bacteroidesand fungi like Wallemia hederae and Codosiga hollandica are key candidate microbes for kanef degumming.

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Screening a bacterium and its effect on the biological degumming of ramie and kenaf

Cited by 20 publications

References 21 publications

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Insights on bio-degumming of kenaf bast based on metagenomic and proteomics

Insights on bio-degumming of kenaf bast based on metagenomic and proteomics

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