Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids

Abstract

The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10 −7 to 10 −14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.

Molecular diversity and microsatellite polymorphism of modern maize hybrids

“…Kozhukhova and Sivolap (2004) differentiated 40 unique molecular patterns of 40 maize genotypes with ten polymorphic SSR loci. In another study, 20 microsatellites revealed unique allelic combinations for 48 commercial maize single-cross hybrids, with a probability of identity ranging from 10 − 14 to 10 − 7 (Ribeiro et al, 2017).…”

Evidence of genetic diversity within Solanum Lycopersicum L. ‘Platense’ landrace and identification of various subpopulations

“…which indicates the power to unambiguously identify a given individual or subpopulation among all others in the set, using the 16 SSR markers described in this study (Ribeiro et al 2017).…”