Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

Arena, Gabriella Dias; Ramos-González, Pedro Luis; Nunes, Maria Andréia; Chabi-Jesus, Camila; Calegario, Renata Faier; Kitajima, Elliot Watanabe; Novelli, Valdenice M.; Freitas-Astúa, Juliana

doi:10.1590/1678-992x-2015-0380

Cited by 15 publications

(9 citation statements)

References 17 publications

(14 reference statements)

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“…phoenicis sensu stricto mites from sweet orange to sweet orange plants as well as to the experimental host A. thaliana, as reported for CoRSV and ClCSV (Arena et al, 2017). Similar to what was observed for these viruses, the time of symptom appearance ranged from 8 to 10 days, and the symptoms appeared as localized lesions.…”

Section: Discussionsupporting

confidence: 76%

“…Similar to what was observed for these viruses, the time of symptom appearance ranged from 8 to 10 days, and the symptoms appeared as localized lesions. However, CiLV-N induced the appearance of typical pinpoint lesions, smaller than those observed for the other two viruses (Arena et al 2017). The infection of Arabidopsis by this virus opens new doors for understanding the interactions between this host, a CL-N-associated virus, and the Brevipalpus mite, as established for the CL-C pathosystem .…”

Section: Discussionmentioning

confidence: 79%

“…from either orchid or citrus) by RT-PCR, a thorough biological and molecular characterization of the Brazilian CL-N pathogen was per-formed. Taken together, the following features placed it within the dichorhavirids: (i) induction of typical localized chlorotic or necrotic leprosis-like symptoms in sweet orange plants; (ii) presence of short rod-like virions of approximately 40 to 100 nm in length, spoke-wheel structure, or electron-lucent viroplasms in infected cells analyzed by TEM; (iii) Brevipalpus mite transmission to both sweet orange and A. thaliana plants yielding localized lesions in the latter, which was recently shown to be a good experimental host for at least two other dichorhaviruses (CoRSV and the tentative dichorhavirids Clerodendrum chlorotic spot virus[ClCSV])(Arena et al, 2017); and (iv) genome size and organization similar to that of dichorhaviruses. This newly sequenced virus has a bipartite (-)ssRNA genome of approximately 6.7 and 6.0 kb for each molecule, of which the RNA1 encodes five ORF, equivalent to genes N, P, MP, M, and G of rhabdovirids, whereas the RNA2 accommodates a single ORF, corresponding to the L gene.…”

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confidence: 99%

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IIdentification, characterization, and evolutionary features of >i<Brevipalpus>/i<-transmitted viruses causing citrus leprosis disease

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 79%

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IIdentification, characterization, and evolutionary features of >i<Brevipalpus>/i<-transmitted viruses causing citrus leprosis disease

Jesus¹

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“…), orchid (Oncidium spp.) (Kitajima et al, 2010), Arabidopsis thaliana (Arena et al, 2017), and Phaseolus vulgaris FIGURE 2 | Phylogenetic analysis of P15 proteins and intra-species variability of ORF p15 from cileviruses. Nucleotide sequence alignments were performed using the MAFFT algorithm (Katoh and Standley, 2013).…”

Section: Open Reading Frame P15 Displays Synonymous Codon Usage Bias That Might Reflect Adaption To Host-specific Codon Usage Patternsmentioning

confidence: 99%

Poorly Conserved P15 Proteins of Cileviruses Retain Elements of Common Ancestry and Putative Functionality: A Theoretical Assessment on the Evolution of Cilevirus Genomes

et al. 2021

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The genus Cilevirus groups enveloped single-stranded (+) RNA virus members of the family Kitaviridae, order Martellivirales. Proteins P15, scarcely conserved polypeptides encoded by cileviruses, have no apparent homologs in public databases. Accordingly, the open reading frames (ORFs) p15, located at the 5′-end of the viral RNA2 molecules, are considered orphan genes (ORFans). In this study, we have delved into ORFs p15 and the relatively poorly understood biochemical properties of the proteins P15 to posit their importance for viruses across the genus and theorize on their origin. We detected that the ORFs p15 are under purifying selection and that, in some viral strains, the use of synonymous codons is biased, which might be a sign of adaptation to their plant hosts. Despite the high amino acid sequence divergence, proteins P15 show the conserved motif [FY]-L-x(3)-[FL]-H-x-x-[LIV]-S-C-x-C-x(2)-C-x-G-x-C, which occurs exclusively in members of this protein family. Proteins P15 also show a common predicted 3D structure that resembles the helical scaffold of the protein ORF49 encoded by radinoviruses and the phosphoprotein C-terminal domain of mononegavirids. Based on the 3D structural similarities of P15, we suggest elements of common ancestry, conserved functionality, and relevant amino acid residues. We conclude by postulating a plausible evolutionary trajectory of ORFans p15 and the 5′-end of the RNA2 of cileviruses considering both protein fold superpositions and comparative genomic analyses with the closest kitaviruses, negeviruses, nege/kita-like viruses, and unrelated viruses that share the ecological niches of cileviruses.

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“…While sweet oranges show high susceptibility, mandarins ( C. reshni , C. reticulata , and C. deliciosa ) are moderately resistant, and lemons ( C. limon ) and limes ( C. aurantifolia ) are considered resistant ( Bastianel et al, 2018 ). CiLV-C also naturally infects Commelina benghalensis and Swinglea glutinosa and can be experimentally transmitted to plants of 28 families ( León et al, 2008 ; Nunes et al, 2012 ; Garita et al, 2014 ; Arena et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas

et al. 2021

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Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.

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Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

Cited by 15 publications

References 17 publications

IIdentification, characterization, and evolutionary features of >i<Brevipalpus>/i<-transmitted viruses causing citrus leprosis disease

IIdentification, characterization, and evolutionary features of >i<Brevipalpus>/i<-transmitted viruses causing citrus leprosis disease

Poorly Conserved P15 Proteins of Cileviruses Retain Elements of Common Ancestry and Putative Functionality: A Theoretical Assessment on the Evolution of Cilevirus Genomes

Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas

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