Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases

Abstract

Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) to trace the animal protein in the blood of 15 buffaloes ( Bubalus bubalis ) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed). Results: On the 36th day, differences were detectable in the feeding profile (p <0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p> 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of 13 C and 15 N was observed through incorporation of the diet into the AVP and AVPR groups. Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use.

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…Therefore, a group of Brazilian researchers from the Center for the Study of Venoms and Venomous Animals (CEVAP) of São Paulo State University (UNESP), Brazil, proposed a new heterologous fibrin sealant (HFS) using a snake venom fraction extracted from Crotalus durissus terrificus and a cryoprecipitate rich in fibrinogen obtained from Bubalus bubalis buffaloes blood [11,17-19].…”

Fibrin Biopolymer Incorporated with Antimicrobial Agents: A Proposal for Coating Denture Bases

“…CEVAP researchers extract the cryoprecipitate, which is then analyzed by two-dimensional electrophoresis, to isolate and identify proteins. The research bases for processing and traceability were reported by Pontes et al, 2017 [ 52 ] and Ferreira Jr. et al, 2019 [ 53 ]. The specifications of formulation are protected by patents no.…”

The utilisation of European processed animal proteins as safe, sustainable and circular ingredients for global aquafeeds

“…85 In more recent times, lab-based traceability tools have been developed and tested as effective at identifying the dietary history of the final fish product. For example, the use of carbon and nitrogen ratio isotopes within the different amino acids of the fish muscle was able to discern the dietary feeding history of a farmed fish, [86][87][88] while the study of protein composition (proteomics) in the feed ingredient and feeds, or even the final fish product could be another means of identifying their origin. 89 Overall, these tools could be deployed into the aquaculture industry by validating production processes.…”